Invitrogen

Cdc7 Monoclonal Antibody (DCS-341)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 References

View all (13) CDC7 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Cdc7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human tonsil stained with CDC 7 antibody using UltraVision LP kit and AEC chromogen. Note nuclear staining of lymphocytes.

Cdc7 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), MCF-7 (Lane 2), A-431 (Lane 3), NIH/3T3 (Lane 4),RAW 264.7 (Lane 5), U-2 OS (Lane 6) and Raji (Lane 7). The blots were probed with Mouse Anti-Cdc7 Monoclonal Antibody (Product # MA5-12589, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 63 kDa corresponding to Cdc7 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Cdc7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with Cdc7 siRNA and loss of signal was observed in immunofluorescence application using Cdc7 Mouse Monoclonal antibody (Product # MA5-12589 ).

Product Details

MA5-12589

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DCS-341

Immunogen

GST fusion protein full length CDC7

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10982416

Product Specific Information

MA5-12589 targets CDC7 Kinase in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-12589 immunogen is gST fusion protein full length CDC7.

Target Information

This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased Cdc7 expression is a marker of oral squamous cell carcinoma and overexpression of Cdc7 contributes to the resistance to DNA-damaging agents.

Cancer letters

Cheng AN,Jiang SS,Fan CC,Lo YK,Kuo CY,Chen CH,Liu YL,Lee CC,Chen WS,Huang TS,Wang TY,Lee AY

MA5-12589 was used in immunohistochemistry and western blot to study the utility of Csc7 as a marker of squamous cell carcinoma and its role in resistance to genotoxic therapeutic agents

Sun Sep 01 00:00:00 EDT 2013

Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.

The Journal of biological chemistry

Lee AY,Chiba T,Truong LN,Cheng AN,Do J,Cho MJ,Chen L,Wu X

MA5-12589 was used in Western Blotting to indicate that DDK is a direct substrate of ATM and ATR to mediate the intra-S-phase checkpoint in mammalian cells.

Fri Jan 20 00:00:00 EST 2012

Geminin overexpression prevents the completion of topoisomerase IIα chromosome decatenation, leading to aneuploidy in human mammary epithelial cells.

Breast cancer research : BCR

Gardner L,Malik R,Shimizu Y,Mullins N,ElShamy WM

MA5-12589 was used in immunoprecipitation and western blot to study the role of geminin in topoisomerase II chromosome decatenation and aneuploidy in human mammary epithelial cells

Thu May 19 00:00:00 EDT 2011

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity.

The Journal of biological chemistry

Hughes S,Jenkins V,Dar MJ,Engelman A,Cherepanov P

MA5-12589 was used in western blot to study the interaction between lens epithelium-derived growth factor and Cdc7-activator of S-phase kinase

Fri Jan 01 00:00:00 EST 2010

Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells.

Journal of cellular biochemistry

Charych DH,Coyne M,Yabannavar A,Narberes J,Chow S,Wallroth M,Shafer C,Walter AO

MA5-12589 was used in western blot to study the ability of Cdc7/Dbf4 kinase to phosphorylate two novel sites on MCM2 in cancer cells

Sun Jun 01 00:00:00 EDT 2008

Geminin is bound to chromatin in G2/M phase to promote proper cytokinesis.

The international journal of biochemistry & cell biology

Nakuci E,Xu M,Pujana MA,Valls J,Elshamy WM

MA5-12589 was used in western blot to study the importance of G2/M phase geminin-chromatin binding for correct cytokinesis

Mon Aug 28 00:00:00 EDT 2006

Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases.

The Journal of biological chemistry

Montagnoli A,Valsasina B,Brotherton D,Troiani S,Rainoldi S,Tenca P,Molinari A,Santocanale C

MA5-12589 was used in western blot to investigate Mcm2 phosphorylation sites by different kinases involved in regulation of S phase progression

Fri Apr 14 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; Cdc7 Kinase; CDC7-like 1; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; MGC117361; MGC126237; MGC126238; MGC91067

Gene Aliases: CDC7; CDC7L1; HsCDC7; Hsk1; huCDC7

UniProt ID: (Human) O00311

Entrez Gene ID: (Human) 8317

Molecular Function: non-receptor serine/threonine protein kinase