|Tested species reactivity||Chicken, Guinea pig, Human, Mouse, Rodent, Rat, Xenopus|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||C-Terminal 2/3 of Xenopus CDC2 expressed in E. coli|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11469 targets Cdk1 in IF, IHC (P), IP, and WB applications and shows reactivity with Chicken, Guinea Pig, Human, mouse, Rodent, Rat, and Xenopus laevis samples.
The MA5-11469 immunogen is c-Terminal 2/3 of Xenopus CDC2 expressed in E. coli.
p34cdc2(a catalytic subunit of Maturation Promoting Factor) plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activated Akt as an indicator of prognosis in gastric cancer.
MA5-11469 was used in immunohistochemistry to examine the prognostic significance of activated AKT in gastric cancer
|Cinti C,Vindigni C,Zamparelli A,La Sala D,Epistolato MC,Marrelli D,Cevenini G,Tosi P||Virchows Archiv : an international journal of pathology (453:449)||2008|
Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.
MA5-11469 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors
|Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M||Human pathology (36:828)||2005|
Expression of the ALK protein by anaplastic large-cell lymphomas correlates with high proliferative activity.
MA5-11469 was used in immunohistochemistry to study the correlation between ALK protein expression and the proliferative capacity of anaplastic large-cell lymphomas
|Leoncini L,Lazzi S,Scano D,Mura A,Onida A,Massarelli G,Tosi P,Barbini P,Cevenini G,Massai MR,Pileri S,Falini B,Giordano A,Kraft R,Laissue JA,Cottier H||International journal of cancer (86:777)||2000|
Retinoblastoma-related p107 and pRb2/p130 proteins in malignant lymphomas: distinct mechanisms of cell growth control.
MA5-11469 was used in immunohistochemistry to study the expression and role of retinoblastoma protein/p107 and retinoblastoma protein2/p130 in non-Hodgkin's lymphoma
|Leoncini L,Bellan C,Cossu A,Claudio PP,Lazzi S,Cinti C,Cevenini G,Megha T,Laurini L,Luzi P,Orcioni GF,Piccioli M,Pileri S,Giardino C,Tosi P,Giordano A||Clinical cancer research : an official journal of the American Association for Cancer Research (5:4065)||1999|
Expression of p34(cdc2) and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: a multivariate cluster analysis.
MA5-11469 was used in immunohistochemistry to perform a multivariate cluster analysis of p34(cdc2), cyclin A and cyclin B expresssion in non-Hodgkin's lymphoma
|Leoncini L,Cossu A,Megha T,Bellan C,Lazzi S,Luzi P,Tosi P,Barbini P,Cevenini G,Pileri S,Giordano A,Kraft R,Laissue JA,Cottier H||International journal of cancer (83:203)||1999|
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
MA5-11469 was used in western blot to study the antiproliferative and apoptotic effects of ixocarpalactone A on colon cancer cells
|Choi JK,Murillo G,Su BN,Pezzuto JM,Kinghorn AD,Mehta RG||The FEBS journal (273:5714)||2006|
In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.
MA5-11469 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma
|Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P||Clinical cancer research : an official journal of the American Association for Cancer Research (11:4875)||2005|
c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.
MA5-11469 was used in western blot to study the effect of c-Myc on DNA damage-induced G2/M arrest.
|Sheen JH,Woo JK,Dickson RB||British journal of cancer (89:1479)||2003|
A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells.
MA5-11469 was used in western blot to study the role cyclin-dependent protein kinase 5 in modulating cAMP-induced apoptosis in rat leukemia cells
|Sandal T,Stapnes C,Kleivdal H,Hedin L,Døskeland SO||The Journal of biological chemistry (277:20783)||2002|
Mitotic cell cycle proteins increase in podocytes despite lack of proliferation.
MA5-11469 was used in immunocytochemistry to study the increase in mitotic cell cycle protein in podocyes in the absence of increased proliferation
|Petermann AT,Pippin J,Hiromura K,Monkawa T,Durvasula R,Couser WG,Kopp J,Shankland SJ||Kidney international (63:113)||2003|