Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Cdk2 (green) in HeLa cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Cdk2 monoclonal antibody (Product # MA5-13725), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human, Mouse, Primate, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse|
|Clone||2B6 + 8D4|
|Immunogen||Human recombinant cdk2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13725 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation; and reacts with human, primate, mouse, and rat samples.
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Relationship of immunohistochemical biomarker expression and lymph node involvement in patients undergoing surgical treatment of NSCLC with long-term follow-up.
MA5-13725 was used in immunohistochemistry to identify biomarkers of lymph node involvement at long-term follow-up in non-small cell lung cancer patients who had been treated surgically
|Gómez AM,Jarabo Sarceda JR,García-Asenjo JA,Fernandez C,Hernandez S,Sanz J,Fernandez E,Calatayud J,Torres A,Hernando F||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (35:4551)||2014|
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.
MA5-13725 was used in immunohistochemistry to study the role of the VRK1 signaling pathway in the proliferation phenotype in head and neck squamous cell carcinoma
|Santos CR,Rodríguez-Pinilla M,Vega FM,Rodríguez-Peralto JL,Blanco S,Sevilla A,Valbuena A,Hernández T,van Wijnen AJ,Li F,de Alava E,Sánchez-Céspedes M,Lazo PA||Molecular cancer research : MCR (4:177)||2006|
Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.
MA5-13725 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors
|Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M||Human pathology (36:828)||2005|
Phenotypic characterization of BRCA1 and BRCA2 tumors based in a tissue microarray study with 37 immunohistochemical markers.
MA5-13725 was used in immunohistochemistry to study the phenotype of BRCA1 and BRCA2 tumors using a tissue microarray with 37 immunohistochemical markers
|Palacios J,Honrado E,Osorio A,Cazorla A,Sarrió D,Barroso A,Rodríguez S,Cigudosa JC,Diez O,Alonso C,Lerma E,Dopazo J,Rivas C,Benítez J||Breast cancer research and treatment (90:5)||2005|
Building an outcome predictor model for diffuse large B-cell lymphoma.
MA5-13725 was used in immunohistochemistry to develop a protein expression-based model for predicting outcome in patients with diffuse large B-cell lymphoma
|Sáez AI,Sáez AJ,Artiga MJ,Pérez-Rosado A,Camacho FI,Díez A,García JF,Fraga M,Bosch R,Rodríguez-Pinilla SM,Mollejo M,Romero C,Sánchez-Verde L,Pollán M,Piris MA||The American journal of pathology (164:613)||2004|
Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays.
MA5-13725 was used in immunohistochemistry to study alterations in tumor suppressor pathways and cell cycle checkpoints in Hodgkin and Reed-Sternberg cells
|García JF,Camacho FI,Morente M,Fraga M,Montalbán C,Alvaro T,Bellas C,Castaño A,Díez A,Flores T,Martin C,Martinez MA,Mazorra F,Menárguez J,Mestre MJ,Mollejo M,Sáez AI,Sánchez L,Piris MA||Blood (101:681)||2003|
Reversible, interrelated mRNA and miRNA expression patterns in the transcriptome of Rasless fibroblasts: functional and mechanistic implications.
MA5-13725 was used in western blot to study the mRNA and miRNA expression profile of 'Rasless' fibroblasts
|Azrak SS,Ginel-Picardo A,Drosten M,Barbacid M,Santos E||BMC genomics (14:null)||2014|
Recruitment of trimeric proliferating cell nuclear antigen by G1-phase cyclin-dependent kinases following DNA damage with platinum-based antitumour agents.
MA5-13725 was used in western blot to study the role of trimeric PCNA recruited to G1-phase Cdk-p21 complexes following platinum-induced DNA damage
|He G,Kuang J,Koomen J,Kobayashi R,Khokhar AR,Siddik ZH||British journal of cancer (109:2378)||2013|
Interference with ER-α enhances the therapeutic efficacy of the selective CDK inhibitor roscovitine towards ER-positive breast cancer cells.
MA5-13725 was used in western blot to investigate the efficacy of roscovitine for breast cancer treatment
|Węsierska-Gądek J,Gritsch D,Zulehner N,Komina O,Maurer M||Journal of cellular biochemistry (112:1103)||2011|
Regulation of p21, MMP-1, and MDR-1 expression in human colon carcinoma HT29 cells by Tian Xian liquid, a chinese medicinal formula, in vitro and in vivo.
MA5-13725 was used in western blot to investigate the effect of Tian-Xian liquid on HT29 cells
|Sze SC,Wong KL,Liu WK,Ng TB,Wong JH,Cheung HP,Yow CM,Chu ES,Hu YM,Tsang KW,Lee WS||Integrative cancer therapies (10:58)||2011|
R-roscovitine (Seliciclib) affects CLL cells more strongly than combinations of fludarabine or cladribine with cyclophosphamide: Inhibition of CDK7 sensitizes leukemic cells to caspase-dependent apoptosis.
MA5-13725 was used in western blot to compare the therapeutic efficacy between R-roscovitine and combinations of fludarabine or cladribine with cyclophosphamide for the treatment of chronic lymphocytic leukemia
|Rogalińska M,Błoński JZ,Komina O,Góralski P,Zołnierczyk JD,Piekarski H,Robak T,Kiliańska ZM,Wesierska-Gadek J||Journal of cellular biochemistry (109:217)||2010|
Impact of roscovitine, a selective CDK inhibitor, on cancer cells: bi-functionality increases its therapeutic potential.
MA5-13725 was used in western blot to exmaine the effect of selective CDK inhibitor roscovitine in cancer cells
|Wesierska-Gadek J,Borza A,Komina O,Maurer M||Acta biochimica Polonica (56:495)||2009|
Outcome of treatment of human HeLa cervical cancer cells with roscovitine strongly depends on the dosage and cell cycle status prior to the treatment.
MA5-13725 was used in western blot to examine the effect of cyclin-dependent kinase inhibitor roscovitine on human HeLa cervical cancer cells
|Wesierska-Gadek J,Borza A,Walzi E,Krystof V,Maurer M,Komina O,Wandl S||Journal of cellular biochemistry (106:937)||2009|
Action of resveratrol alone or in combination with roscovitine, a CDK inhibitor, on cell cycle progression in human HL-60 leukemia cells.
MA5-13725 was used in western blot to investigate the effect of resveratrol alone or in combination with roscovitine on cell cycle progression in human HL-60 leukemia cells
|Komina O,Wesierska-Gadek J||Biochemical pharmacology (76:1554)||2008|
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
MA5-13725 was used in western blot to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis
|Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L||Hepatology (Baltimore, Md.) (48:289)||2008|
The c-Jun N-terminal kinase pathway is critical for cell transformation by the latent membrane protein 1 of Epstein-Barr virus.
MA5-13725 was used in western blot to study the mechanism of cell transformation by latent membrane protein 1 of Epstein-Barr virus
|Kutz H,Reisbach G,Schultheiss U,Kieser A||Virology (371:246)||2008|
Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.
MA5-13725 was used in western blot to study the distinct mechanisms by which muscadine grape skin extract and resveratrol inhibit prostate cancer cell growth
|Hudson TS,Hartle DK,Hursting SD,Nunez NP,Wang TT,Young HA,Arany P,Green JE||Cancer research (67:8396)||2007|
Apigenin-induced cell cycle arrest is mediated by modulation of MAPK, PI3K-Akt, and loss of cyclin D1 associated retinoblastoma dephosphorylation in human prostate cancer cells.
MA5-13725 was used in western blot to study the mechanism of apigenin-induced cell cycle arrest in human prostate cancer cells
|Shukla S,Gupta S||Cell cycle (Georgetown, Tex.) (6:1102)||2007|
Overexpression of cyclins D1 and D3 during estrogen-induced breast oncogenesis in female ACI rats.
MA5-13725 was used in western blot to study the expression of cyclin D1 and cyclin D3 during estrogen-induced mammary tumorigenesis in rats
|Weroha SJ,Li SA,Tawfik O,Li JJ||Carcinogenesis (27:491)||2006|
Differential regulation of CDP/Cux p110 by cyclin A/Cdk2 and cyclin A/Cdk1.
MA5-13725 was used in western blot to study the differential regulation of CDP/Cux p110 by cyclin A/cdk2 and cyclin A/cdk1
|Santaguida M,Nepveu A||The Journal of biological chemistry (280:32712)||2005|
In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.
MA5-13725 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma
|Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P||Clinical cancer research : an official journal of the American Association for Cancer Research (11:4875)||2005|
Abolishment of the interaction between cyclin-dependent kinase 2 and Cdk-associated protein phosphatase by a truncated KAP mutant.
MA5-13725 was used in western blot to study the ability of a truncated KAP mutant to abolish the interaction between Cdk2 and Cdk-associated protein phosphatase
|Yeh CT,Lu SC,Chao CH,Chao ML||Biochemical and biophysical research communications (305:311)||2003|
A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells.
MA5-13725 was used in western blot to study the role cyclin-dependent protein kinase 5 in modulating cAMP-induced apoptosis in rat leukemia cells
|Sandal T,Stapnes C,Kleivdal H,Hedin L,Døskeland SO||The Journal of biological chemistry (277:20783)||2002|
Cyclin-dependent kinase-associated protein phosphatase is overexpressed in alcohol-related hepatocellular carcinoma and influences xenograft tumor growth.
MA5-13725 was used in immunoprecipitation and western blot to study the overexpression of KAP in alcohol-related hepatocellular carcinoma and the inhibitorsy effect of KAP knockdown on the growth of tumor xenografts
|Lin WR,Lai MW,Yeh CT||Oncology reports (29:903)||2013|
Overexpression of cyclin-dependent kinase-associated protein phosphatase enhances cell proliferation in renal cancer cells.
MA5-13725 was used in immunoprecipitation and western blot to study the role of cyclin-dependent kinase-associated protein phosphatase in renal cancer cell growth
|Lai MW,Chen TC,Pang ST,Yeh CT||Urologic oncology (30:871)||2012|
cdc2-related protein kinase; cell devision kinase 2; cell division protein kinase 2; cyclin dependent kinase 2-alpha; cyclin-dependent kinase 2; p33 protein kinase
A630093N05Rik; CDK2; CDKN2; p33(CDK2)