Immunofluorescent analysis of Cdk2 (green) in HeLa cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Cdk2 monoclonal antibody (Product # MA5-13725), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse|
|Clone||2B6 + 8D4|
|Immunogen||Human recombinant cdk2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13725 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation; and reacts with human, primate, mouse, and rat samples.
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation.
MA5-13725 was used in western blot to characterize inhibition of neuronal tumor cell proliferation by mitochondrial ferritin
|Shi ZH,Shi FF,Wang YQ,Sheftel AD,Nie G,Zhao YS,You LH,Gou YJ,Duan XL,Zhao BL,Xu HM,Li CY,Chang YZ||Cellular and molecular life sciences : CMLS (72:983)||2015|
Recruitment of trimeric proliferating cell nuclear antigen by G1-phase cyclin-dependent kinases following DNA damage with platinum-based antitumour agents.
MA5-13725 was used in western blot to study the role of trimeric PCNA recruited to G1-phase Cdk-p21 complexes following platinum-induced DNA damage
|He G,Kuang J,Koomen J,Kobayashi R,Khokhar AR,Siddik ZH||British journal of cancer (109:2378)||2013|
Reversible, interrelated mRNA and miRNA expression patterns in the transcriptome of Rasless fibroblasts: functional and mechanistic implications.
MA5-13725 was used in western blot to study the mRNA and miRNA expression profile of 'Rasless' fibroblasts
|Azrak SS,Ginel-Picardo A,Drosten M,Barbacid M,Santos E||BMC genomics (14:null)||2013|
Interference with ER-¿ enhances the therapeutic efficacy of the selective CDK inhibitor roscovitine towards ER-positive breast cancer cells.
MA5-13725 was used in western blot to investigate the efficacy of roscovitine for breast cancer treatment
|W¿sierska-G¿dek J,Gritsch D,Zulehner N,Komina O,Maurer M||Journal of cellular biochemistry (112:1103)||2011|
Regulation of p21, MMP-1, and MDR-1 expression in human colon carcinoma HT29 cells by Tian Xian liquid, a chinese medicinal formula, in vitro and in vivo.
MA5-13725 was used in western blot to investigate the effect of Tian-Xian liquid on HT29 cells
|Sze SC,Wong KL,Liu WK,Ng TB,Wong JH,Cheung HP,Yow CM,Chu ES,Hu YM,Tsang KW,Lee WS||Integrative cancer therapies (10:58)||2011|
R-roscovitine (Seliciclib) affects CLL cells more strongly than combinations of fludarabine or cladribine with cyclophosphamide: Inhibition of CDK7 sensitizes leukemic cells to caspase-dependent apoptosis.
MA5-13725 was used in western blot to compare the therapeutic efficacy between R-roscovitine and combinations of fludarabine or cladribine with cyclophosphamide for the treatment of chronic lymphocytic leukemia
|Rogali¿ska M,B¿o¿ski JZ,Komina O,Góralski P,Zo¿nierczyk JD,Piekarski H,Robak T,Kilia¿ska ZM,Wesierska-Gadek J||Journal of cellular biochemistry (109:217)||2010|
E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin.
MA5-13725 was used in western blot to investigate the cellular effects of chlorophyllin treatment
|Chimploy K,Díaz GD,Li Q,Carter O,Dashwood WM,Mathews CK,Williams DE,Bailey GS,Dashwood RH||International journal of cancer (125:2086)||2009|
Impact of roscovitine, a selective CDK inhibitor, on cancer cells: bi-functionality increases its therapeutic potential.
MA5-13725 was used in western blot to exmaine the effect of selective CDK inhibitor roscovitine in cancer cells
|Wesierska-Gadek J,Borza A,Komina O,Maurer M||Acta biochimica Polonica (56:495)||2009|
Outcome of treatment of human HeLa cervical cancer cells with roscovitine strongly depends on the dosage and cell cycle status prior to the treatment.
MA5-13725 was used in western blot to examine the effect of cyclin-dependent kinase inhibitor roscovitine on human HeLa cervical cancer cells
|Wesierska-Gadek J,Borza A,Walzi E,Krystof V,Maurer M,Komina O,Wandl S||Journal of cellular biochemistry (106:937)||2009|
Action of resveratrol alone or in combination with roscovitine, a CDK inhibitor, on cell cycle progression in human HL-60 leukemia cells.
MA5-13725 was used in western blot to investigate the effect of resveratrol alone or in combination with roscovitine on cell cycle progression in human HL-60 leukemia cells
|Komina O,Wesierska-Gadek J||Biochemical pharmacology (76:1554)||2008|
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
MA5-13725 was used in western blot to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis
|Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L||Hepatology (Baltimore, Md.) (48:289)||2008|
The c-Jun N-terminal kinase pathway is critical for cell transformation by the latent membrane protein 1 of Epstein-Barr virus.
MA5-13725 was used in western blot to study the mechanism of cell transformation by latent membrane protein 1 of Epstein-Barr virus
|Kutz H,Reisbach G,Schultheiss U,Kieser A||Virology (371:246)||2008|
Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.
MA5-13725 was used in western blot to study the distinct mechanisms by which muscadine grape skin extract and resveratrol inhibit prostate cancer cell growth
|Hudson TS,Hartle DK,Hursting SD,Nunez NP,Wang TT,Young HA,Arany P,Green JE||Cancer research (67:8396)||2007|
Apigenin-induced cell cycle arrest is mediated by modulation of MAPK, PI3K-Akt, and loss of cyclin D1 associated retinoblastoma dephosphorylation in human prostate cancer cells.
MA5-13725 was used in western blot to study the mechanism of apigenin-induced cell cycle arrest in human prostate cancer cells
|Shukla S,Gupta S||Cell cycle (Georgetown, Tex.) (6:1102)||2007|
Overexpression of cyclins D1 and D3 during estrogen-induced breast oncogenesis in female ACI rats.
MA5-13725 was used in western blot to study the expression of cyclin D1 and cyclin D3 during estrogen-induced mammary tumorigenesis in rats
|Weroha SJ,Li SA,Tawfik O,Li JJ||Carcinogenesis (27:491)||2006|
Differential regulation of CDP/Cux p110 by cyclin A/Cdk2 and cyclin A/Cdk1.
MA5-13725 was used in western blot to study the differential regulation of CDP/Cux p110 by cyclin A/cdk2 and cyclin A/cdk1
|Santaguida M,Nepveu A||The Journal of biological chemistry (280:32712)||2005|
In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.
MA5-13725 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma
|Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P||Clinical cancer research : an official journal of the American Association for Cancer Research (11:4875)||2005|
Abolishment of the interaction between cyclin-dependent kinase 2 and Cdk-associated protein phosphatase by a truncated KAP mutant.
MA5-13725 was used in western blot to study the ability of a truncated KAP mutant to abolish the interaction between Cdk2 and Cdk-associated protein phosphatase
|Yeh CT,Lu SC,Chao CH,Chao ML||Biochemical and biophysical research communications (305:311)||2003|
A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells.
MA5-13725 was used in western blot to study the role cyclin-dependent protein kinase 5 in modulating cAMP-induced apoptosis in rat leukemia cells
|Sandal T,Stapnes C,Kleivdal H,Hedin L,Døskeland SO||The Journal of biological chemistry (277:20783)||2002|
Relationship of immunohistochemical biomarker expression and lymph node involvement in patients undergoing surgical treatment of NSCLC with long-term follow-up.
MA5-13725 was used in immunohistochemistry to identify biomarkers of lymph node involvement at long-term follow-up in non-small cell lung cancer patients who had been treated surgically
|Gómez AM,Jarabo Sarceda JR,García-Asenjo JA,Fernandez C,Hernandez S,Sanz J,Fernandez E,Calatayud J,Torres A,Hernando F||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (35:4551)||2014|
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.
MA5-13725 was used in immunohistochemistry to study the role of the VRK1 signaling pathway in the proliferation phenotype in head and neck squamous cell carcinoma
|Santos CR,Rodríguez-Pinilla M,Vega FM,Rodríguez-Peralto JL,Blanco S,Sevilla A,Valbuena A,Hernández T,van Wijnen AJ,Li F,de Alava E,Sánchez-Céspedes M,Lazo PA||Molecular cancer research : MCR (4:177)||2006|
Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.
MA5-13725 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors
|Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M||Human pathology (36:828)||2005|
Phenotypic characterization of BRCA1 and BRCA2 tumors based in a tissue microarray study with 37 immunohistochemical markers.
MA5-13725 was used in immunohistochemistry to study the phenotype of BRCA1 and BRCA2 tumors using a tissue microarray with 37 immunohistochemical markers
|Palacios J,Honrado E,Osorio A,Cazorla A,Sarrió D,Barroso A,Rodríguez S,Cigudosa JC,Diez O,Alonso C,Lerma E,Dopazo J,Rivas C,Benítez J||Breast cancer research and treatment (90:5)||2005|
Building an outcome predictor model for diffuse large B-cell lymphoma.
MA5-13725 was used in immunohistochemistry to develop a protein expression-based model for predicting outcome in patients with diffuse large B-cell lymphoma
|Sáez AI,Sáez AJ,Artiga MJ,Pérez-Rosado A,Camacho FI,Díez A,García JF,Fraga M,Bosch R,Rodríguez-Pinilla SM,Mollejo M,Romero C,Sánchez-Verde L,Pollán M,Piris MA||The American journal of pathology (164:613)||2004|
Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays.
MA5-13725 was used in immunohistochemistry to study alterations in tumor suppressor pathways and cell cycle checkpoints in Hodgkin and Reed-Sternberg cells
|García JF,Camacho FI,Morente M,Fraga M,Montalbán C,Alvaro T,Bellas C,Castaño A,Díez A,Flores T,Martin C,Martinez MA,Mazorra F,Menárguez J,Mestre MJ,Mollejo M,Sáez AI,Sánchez L,Piris MA||Blood (101:681)||2003|
Cyclin-dependent kinase-associated protein phosphatase is overexpressed in alcohol-related hepatocellular carcinoma and influences xenograft tumor growth.
MA5-13725 was used in immunoprecipitation and western blot to study the overexpression of KAP in alcohol-related hepatocellular carcinoma and the inhibitorsy effect of KAP knockdown on the growth of tumor xenografts
|Lin WR,Lai MW,Yeh CT||Oncology reports (29:903)||2013|
Overexpression of cyclin-dependent kinase-associated protein phosphatase enhances cell proliferation in renal cancer cells.
MA5-13725 was used in immunoprecipitation and western blot to study the role of cyclin-dependent kinase-associated protein phosphatase in renal cancer cell growth
|Lai MW,Chen TC,Pang ST,Yeh CT||Urologic oncology (30:871)||2012|
Bottom-up cell proliferation with cyclin A and p27Kip1 expression in ulcerative colitis-associated dysplasia.
MA5-13725 was used in immunohistochemistry - paraffin section to examine the cell kinetics of ulcerative colitis-associated dysplasia and compare it to sporadic tubular adenoma
|Mikami T,Yoshida T,Shiraishi H,Tokuyama W,Motoori T,Okayasu I||Pathology international (56:10)||2006|
Progression in cutaneous malignant melanoma is associated with distinct expression profiles: a tissue microarray-based study.
MA5-13725 was used in immunohistochemistry (paraffin) to characterize cutaneous malignant melanoma samples.
|Alonso SR,Ortiz P,Pollán M,Pérez-Gómez B,Sánchez L,Acuña MJ,Pajares R,Martínez-Tello FJ,Hortelano CM,Piris MA,Rodríguez-Peralto JL||The American journal of pathology (164:193)||2004|