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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human recombinant cdk2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-50|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13476 targets Cdk2 in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13476 immunogen is human recombinant cdk2 protein.
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Menthol inhibits the proliferation and motility of prostate cancer DU145 cells.
MA5-13476 was used in western blot to study the effects of TRPM8 activation on prostate cancer cell proliferation and migration
|Wang Y,Wang X,Yang Z,Zhu G,Chen D,Meng Z||Pathology oncology research : POR (18:903)||2012|
DCPIB, a specific inhibitor of volume-regulated anion channels (VRACs), inhibits astrocyte proliferation and cell cycle progression via G1/S arrest.
MA5-13476 was used in western blot to study the blockade of astrocyte proliferation and cell cycle progression by a VRAC inhibitor
|He D,Luo X,Wei W,Xie M,Wang W,Yu Z||Journal of molecular neuroscience : MN (46:249)||2012|
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
MA5-13476 was used in western blot to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis
|Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L||Hepatology (Baltimore, Md.) (48:289)||2008|
The c-Jun N-terminal kinase pathway is critical for cell transformation by the latent membrane protein 1 of Epstein-Barr virus.
MA5-13476 was used in western blot to study the mechanism of cell transformation by latent membrane protein 1 of Epstein-Barr virus
|Kutz H,Reisbach G,Schultheiss U,Kieser A||Virology (371:246)||2008|
Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.
MA5-13476 was used in western blot to study the distinct mechanisms by which muscadine grape skin extract and resveratrol inhibit prostate cancer cell growth
|Hudson TS,Hartle DK,Hursting SD,Nunez NP,Wang TT,Young HA,Arany P,Green JE||Cancer research (67:8396)||2007|
Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells.
MA5-13476 was used in western blot to investigate the correlation between nuclear localization of barrier-to-autointegration factor and S phase progression in human cells
|Haraguchi T,Koujin T,Osakada H,Kojidani T,Mori C,Masuda H,Hiraoka Y||Journal of cell science (120:1967)||2007|
Apigenin-induced cell cycle arrest is mediated by modulation of MAPK, PI3K-Akt, and loss of cyclin D1 associated retinoblastoma dephosphorylation in human prostate cancer cells.
MA5-13476 was used in western blot to study the mechanism of apigenin-induced cell cycle arrest in human prostate cancer cells
|Shukla S,Gupta S||Cell cycle (Georgetown, Tex.) (6:1102)||2007|
Overexpression of cyclins D1 and D3 during estrogen-induced breast oncogenesis in female ACI rats.
MA5-13476 was used in western blot to study the expression of cyclin D1 and cyclin D3 during estrogen-induced mammary tumorigenesis in rats
|Weroha SJ,Li SA,Tawfik O,Li JJ||Carcinogenesis (27:491)||2006|
In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.
MA5-13476 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma
|Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P||Clinical cancer research : an official journal of the American Association for Cancer Research (11:4875)||2005|
Abolishment of the interaction between cyclin-dependent kinase 2 and Cdk-associated protein phosphatase by a truncated KAP mutant.
MA5-13476 was used in western blot to study the ability of a truncated KAP mutant to abolish the interaction between Cdk2 and Cdk-associated protein phosphatase
|Yeh CT,Lu SC,Chao CH,Chao ML||Biochemical and biophysical research communications (305:311)||2003|
A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells.
MA5-13476 was used in western blot to study the role cyclin-dependent protein kinase 5 in modulating cAMP-induced apoptosis in rat leukemia cells
|Sandal T,Stapnes C,Kleivdal H,Hedin L,Døskeland SO||The Journal of biological chemistry (277:20783)||2002|
Proteomic analysis of waldenstrom macroglobulinemia.
MA5-13476 was used in immunohistochemistry to profile the changes in protein expression in waldenstrom macroglobulinemia
|Hatjiharissi E,Ngo H,Leontovich AA,Leleu X,Timm M,Melhem M,George D,Lu G,Ghobrial J,Alsayed Y,Zeismer S,Cabanela M,Nehme A,Jia X,Moreau AS,Treon SP,Fonseca R,Gertz MA,Anderson KC,Witzig TE,Ghobrial IM||Cancer research (67:3777)||2007|
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.
MA5-13476 was used in immunohistochemistry to study the role of the VRK1 signaling pathway in the proliferation phenotype in head and neck squamous cell carcinoma
|Santos CR,Rodríguez-Pinilla M,Vega FM,Rodríguez-Peralto JL,Blanco S,Sevilla A,Valbuena A,Hernández T,van Wijnen AJ,Li F,de Alava E,Sánchez-Céspedes M,Lazo PA||Molecular cancer research : MCR (4:177)||2006|
Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.
MA5-13476 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors
|Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M||Human pathology (36:828)||2005|
Phenotypic characterization of BRCA1 and BRCA2 tumors based in a tissue microarray study with 37 immunohistochemical markers.
MA5-13476 was used in immunohistochemistry to study the phenotype of BRCA1 and BRCA2 tumors using a tissue microarray with 37 immunohistochemical markers
|Palacios J,Honrado E,Osorio A,Cazorla A,Sarrió D,Barroso A,Rodríguez S,Cigudosa JC,Diez O,Alonso C,Lerma E,Dopazo J,Rivas C,Benítez J||Breast cancer research and treatment (90:5)||2005|
Building an outcome predictor model for diffuse large B-cell lymphoma.
MA5-13476 was used in immunohistochemistry to develop a protein expression-based model for predicting outcome in patients with diffuse large B-cell lymphoma
|Sáez AI,Sáez AJ,Artiga MJ,Pérez-Rosado A,Camacho FI,Díez A,García JF,Fraga M,Bosch R,Rodríguez-Pinilla SM,Mollejo M,Romero C,Sánchez-Verde L,Pollán M,Piris MA||The American journal of pathology (164:613)||2004|
Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays.
MA5-13476 was used in immunohistochemistry to study alterations in tumor suppressor pathways and cell cycle checkpoints in Hodgkin and Reed-Sternberg cells
|García JF,Camacho FI,Morente M,Fraga M,Montalbán C,Alvaro T,Bellas C,Castaño A,Díez A,Flores T,Martin C,Martinez MA,Mazorra F,Menárguez J,Mestre MJ,Mollejo M,Sáez AI,Sánchez L,Piris MA||Blood (101:681)||2003|
cdc2-related protein kinase; cell devision kinase 2; cell division protein kinase 2; cyclin dependent kinase 2-alpha; cyclin-dependent kinase 2; p33 protein kinase
A630093N05Rik; CDK2; CDKN2; p33(CDK2)