Immunofluorescence analysis of Cdk5 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cdk5 (DC17 + DC34) Mouse Monoclonal Antibody (MA511291) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Clone||DC17 + DC34|
|Immunogen||Recombinant cdk5 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||6 ug/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11291 targets Cdk5 in IF, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-11291 immunogen is recombinant cdk5 protein.
Cdk5 is expressed in both cycling and noncycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. The p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclin-dependent kinase-5 and p35/p25 activators in schizophrenia and major depression prefrontal cortex: basal contents and effects of psychotropic medications.
MA5-11291 was used in western blot to study the expression of CDK5, p35 and p25 in the prefrontal cortex of individuals with schizophrenia or major depression
|Ramos-Miguel A,Meana JJ,García-Sevilla JA||The international journal of neuropsychopharmacology (16:683)||2013|
Crosstalk between cdk5 and MEK-ERK signalling upon opioid receptor stimulation leads to upregulation of activator p25 and MEK1 inhibition in rat brain.
MA5-11291 was used in western blot to study the mechanism by which opioid receptor stimulation leads to upregulation of activator p25 and MEK1 inhibition in rat brain
|Ramos-Miguel A,García-Sevilla JA||Neuroscience (215:17)||2012|
The protective effects of tanshinone IIA on neurotoxicity induced by ß-amyloid protein through calpain and the p35/Cdk5 pathway in primary cortical neurons.
MA5-11291 was used in immunocytochemistry and western blot to study the roles of calpain, p35 and Cdk5 in the mechanism by which tanshinone IIA protects primary cortical neurons against the neurotoxic effects of Abeta (25-35)
|Shi LL,Yang WN,Chen XL,Zhang JS,Yang PB,Hu XD,Han H,Qian YH,Liu Y||Neurochemistry international (61:227)||2012|
(+/-)-huprine Y, (-)-huperzine A and tacrine do not show neuroprotective properties in an apoptotic model of neuronal cytoskeletal alteration.
MA5-11291 was used in immunocytochemistry to study the effect of acetylcholinesterase inhibitors in colchicine-induced apoptosis in cerebellar granule neurons
|Jordá EG,Verdaguer E,Jiménez A,Canudas AM,Rimbau V,Camps P,Muñoz-Torrero D,Camins A,Pallàs M||Journal of Alzheimer's disease : JAD (6:577)||2004|