|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Avian, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant cdk6 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13330 targets Cdk6 in ICC/IF, IHC (F, P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13330 immunogen is purified recombinant cdk6 protein.
Cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE) belongs to a family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Menthol inhibits the proliferation and motility of prostate cancer DU145 cells.
MA5-13330 was used in western blot to study the effects of TRPM8 activation on prostate cancer cell proliferation and migration
|Wang Y,Wang X,Yang Z,Zhu G,Chen D,Meng Z||Pathology oncology research : POR (18:903)||2012|
Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture.
MA5-13330 was used in western blot to study the roles of cyclin D1 and cyclin D3 in different mitogenic modes of human thyrocytes
|Paternot S,Dumont JE,Roger PP||Molecular endocrinology (Baltimore, Md.) (20:3279)||2006|
Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors".
MA5-13330 was used in western blot to study the mechanisms underlying the activating phosphorylation of CDK4 on threonine 172
|Bockstaele L,Kooken H,Libert F,Paternot S,Dumont JE,de Launoit Y,Roger PP,Coulonval K||Molecular and cellular biology (26:5070)||2006|
Growth inhibition of human hepatoma cells by acyclic retinoid is associated with induction of p21(CIP1) and inhibition of expression of cyclin D1.
MA5-13330 was used in western blot to study the mechanism by which acyclic retinoid inhibits the growth of human hepatoma cells
|Suzui M,Masuda M,Lim JT,Albanese C,Pestell RG,Weinstein IB||Cancer research (62:3997)||2002|
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
MA5-13330 was used in immunoprecipitation to study the mechanism by which v-Jun promotes S-phase entry in the absence of mitogens
|Clark W,Black EJ,MacLaren A,Kruse U,LaThangue N,Vogt PK,Gillespie DA||Molecular and cellular biology (20:2529)||2000|