Invitrogen

Centaurin alpha-1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (9) Centaurin alpha-1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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Centaurin alpha-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Centaurin alpha-1 in human cell line A-431 shows positivity in plasma membrane & cytoplasm. Samples were probed using a Centaurin alpha-1 Polyclonal Antibody (Product # PA5-52378).

Centaurin alpha-1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Centaurin alpha-1 in human cerebral cortex, hippocampus, ovary and tonsil using Centaurin alpha-1 Polyclonal Antibody (Product # PA5-52378) (A) shows similar protein distribution across tissues to an independent Centaurin alpha-1 Polyclonal Antibody (B).

Centaurin alpha-1 Antibody in Western Blot (WB) — Western blot analysis of Centaurin alpha-1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a Centaurin alpha-1 Polyclonal Antibody (Product # PA5-52378).

Centaurin alpha-1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Centaurin alpha-1 demonstrates antibody specificity. Immunohistochemical analysis of Centaurin alpha-1 using anti-Centaurin alpha-1 Polyclonal Antibody (Product # PA5-52378), shows significant staining of Centaurin alpha-1 in human cerebral cortex and shows minimal or weak staining in human tonsil tissues. The relative expression levels of Centaurin alpha-1 within each tissue is shown using RNA-Seq.

Product Details

PA5-52378

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Centaurin alpha-1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2639658

Product Specific Information

Immunogen sequence: PKQTEGFRKR WFTMDDRRLM YFKDPLDAFA RGEVFIGSKE SGYTVLHGFP PSTQGHHWPH GITIVTPDRK FLFACETESD QREWVAAFQK AVDRPMLPQE YAVE

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

Centaurin alpha-1 (ADAP1, ArfGAP With Dual PH Domains 1) is a Protein Coding gene. Among its related pathways are B Cell Receptor Signaling Pathway (sino) and Arf6 signaling events. Gene Ontology (GO) annotations related to this gene include GTPase activator activity and inositol 1,3,4,5 tetrakisphosphate binding. An important paralog of this gene is ADAP2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Arf-GAP with dual PH domain-containing protein 1; centaurin, alpha 1; centaurin-alpha; Centaurin-alpha-1; Cnt-a1; Putative MAPK-activating protein PM25

Gene Aliases: ADAP1; CENTA1; GCS1L; p42IP4

UniProt ID: (Human) O75689

Entrez Gene ID: (Human) 11033

Function(s)

cell surface receptor signaling pathway regulation of GTPase activity positive regulation of GTPase activity

Process(es)

GTPase activator activity protein binding inositol 1,3,4,5 tetrakisphosphate binding metal ion binding