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|Tested species reactivity||Human, Mouse, Clawed frog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 157-172 of human Centrin 1, with an N-terminal added lysine. This sequence is highly c|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25039 detects Centrin 1 from human samples. PA1-25039 is expected to cross react with mouse, Xenopus laevis, and Drosophilia melanogaster due to sequence homology.
PA1-25039 has been successfully used in Western blot and IF/ICC procedures.
The PA1-25039 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 157-172 of human Centrin 1, with an N-terminal added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, Drosophila, and Xenopus.
The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
calcium binding protein; caltractin; CEN1; centrin, EF-hand protein, 1; centrin, EF-hand protein, 2; centrin-1; CETN; EF-hand protein; testicular tissue protein Li 37; testis-specific murine centrin
Calt; caltractin; CEN1; CETN; CETN1; cetn2