|Tested species reactivity||Chicken|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol, proprietary stabilizer|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Cross Adsorption||Against bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Product SA1-300 is conjugated to HRP and optimized for ECL applications. Product SA1-300 reacts with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins.
Thermo Scientific Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neurosensory and neuromuscular organization in tube feet of the sea urchin Strongylocentrotus purpuratus.
SA1-300 was used in western blot to determine which distinct photosensory structures are present in adult sea urchins
|Agca C,Elhajj MC,Klein WH,Venuti JM||The Journal of comparative neurology (519:3566)||2011|
|Not Applicable||Not Cited||
Krüppel-like factor 4 regulates macrophage polarization.
SA1-300 was used in western blot to examine the role of Kruppel-like factor 4 in macrophage polarization
|Liao X,Sharma N,Kapadia F,Zhou G,Lu Y,Hong H,Paruchuri K,Mahabeleshwar GH,Dalmas E,Venteclef N,Flask CA,Kim J,Doreian BW,Lu KQ,Kaestner KH,Hamik A,Clément K,Jain MK||The Journal of clinical investigation (121:2736)||2011|