|Flow Cytometry (Flow)||1:50 - 1:200|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:50 - 1:200|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Chicken|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||4° C|
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Antibody Specificity: This antibody reacts with the heavy chains on chicken IgY and with the light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins using immunoelectrophoresis. However, these antibodies may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 2-8°C until opened. Restore to 1.1 ml with distilled water (1.5 mg/ml after restoration). Centrifuge product if it is not clear upon standing for 1-2 hours at room temperature. To judge clarity, draw the product into a pasteur pipette. Product may be stored for several weeks at 2-8°C as undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)
Amax = 492 nm; Emax = 520 nm
Fluorophore/Protein: 8.5 µg/mg; 3.2 moles FITC per mole IgG
Country of Origin: USA
Thermo Scientific Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.