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|Tested species reactivity||Chicken|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 555|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.||Zlatic SA,Grossniklaus EJ,Ryder PV,Salazar G,Mattheyses AL,Peden AA,Faundez V||Molecular biology of the cell (24:2378)||2013|
|Not Applicable||Not Cited||Overexpression of facioscapulohumeral muscular dystrophy region gene 1 causes primary defects in myogenic stem cells.||Xynos A,Neguembor MV,Caccia R,Licastro D,Nonis A,Di Serio C,Stupka E,Gabellini D||Journal of cell science (126:2236)||2013|
|Not Applicable||Not Cited||GCC185 plays independent roles in Golgi structure maintenance and AP-1-mediated vesicle tethering.||Brown FC,Schindelhaim CH,Pfeffer SR||The Journal of cell biology (194:779)||2011|
|Not Applicable||Not Cited||Fibroblast growth factor receptor-3 (FGFR-3) regulates expression of paneth cell lineage-specific genes in intestinal epithelial cells through both TCF4/beta-catenin-dependent and -independent signaling pathways.||Brodrick B,Vidrich A,Porter E,Bradley L,Buzan JM,Cohn SM||The Journal of biological chemistry (286:18515)||2011|
|Not Applicable||Not Cited||SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster.||Yan R,Thomas SE,Tsai JH,Yamada Y,McKee BD||The Journal of cell biology (188:335)||2010|