|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 647|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human, mouse and rabbit IgG prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 10 publications below|
This Chicken anti-goat antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from goat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Flourescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||NK cells regulate CD8+ T cell priming and dendritic cell migration during influenza A infection by IFN-¿ and perforin-dependent mechanisms.||Ge MQ,Ho AW,Tang Y,Wong KH,Chua BY,Gasser S,Kemeny DM||Journal of immunology (Baltimore, Md. : 1950) (189:2099)||2012|
|Not Applicable||Not Cited||Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis.||González AM,Garcia T,Samper E,Rickmann M,Vaquero EC,Molero X||American journal of physiology. Gastrointestinal and liver physiology (301:G846)||2011|
|Not Applicable||Not Cited||Role of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191.||Birkus G,Kutty N,Frey CR,Shribata R,Chou T,Wagner C,McDermott M,Cihlar T||Antimicrobial agents and chemotherapy (55:2166)||2011|
|Not Applicable||Not Cited||Tamm-Horsfall protein-deficient thick ascending limbs promote injury to neighboring S3 segments in an MIP-2-dependent mechanism.||El-Achkar TM,McCracken R,Rauchman M,Heitmeier MR,Al-Aly Z,Dagher PC,Wu XR||American journal of physiology. Renal physiology (300:F999)||2011|
|Not Applicable||Not Cited||Timing of bone marrow cell delivery has minimal effects on cell viability and cardiac recovery after myocardial infarction.||Swijnenburg RJ,Govaert JA,van der Bogt KE,Pearl JI,Huang M,Stein W,Hoyt G,Vogel H,Contag CH,Robbins RC,Wu JC||Circulation. Cardiovascular imaging (3:77)||2010|
|Not Applicable||Not Cited||Filamin B mediates ICAM-1-driven leukocyte transendothelial migration.||Kanters E,van Rijssel J,Hensbergen PJ,Hondius D,Mul FP,Deelder AM,Sonnenberg A,van Buul JD,Hordijk PL||The Journal of biological chemistry (283:31830)||2008|
|Not Applicable||Not Cited||Tamm-Horsfall protein protects the kidney from ischemic injury by decreasing inflammation and altering TLR4 expression.||El-Achkar TM,Wu XR,Rauchman M,McCracken R,Kiefer S,Dagher PC||American journal of physiology. Renal physiology (295:F534)||2008|
|Not Applicable||Not Cited||Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc and Tcf3 transcription factors in the thymic stroma.||Popa I,Zubkova I,Medvedovic M,Romantseva T,Mostowski H,Boyd R,Zaitseva M||International immunology (19:1249)||2007|
|Not Applicable||Not Cited||Sepsis induces an increase in thick ascending limb Cox-2 that is TLR4 dependent.||El-Achkar TM,Plotkin Z,Marcic B,Dagher PC||American journal of physiology. Renal physiology (293:F1187)||2007|
|Not Applicable||Not Cited||Cathepsin A is the major hydrolase catalyzing the intracellular hydrolysis of the antiretroviral nucleotide phosphonoamidate prodrugs GS-7340 and GS-9131.||Birkus G,Wang R,Liu X,Kutty N,MacArthur H,Cihlar T,Gibbs C,Swaminathan S,Lee W,McDermott M||Antimicrobial agents and chemotherapy (51:543)||2007|