|Immunocytochemistry (ICC)||4 µg/mL|
|Immunofluorescence (IF)||4 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 6 publications below|
|Host / Isotype||Chicken / IgY|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 647|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage conditions||4° C, store in dark|
|Cross Adsorption||Against human and mouse IgG prior to conjugation|
|Antibody Form||Whole Antibody|
This chicken anti-rabbit antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Flourescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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