|Tested species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/200 - 1/1000|
|Western Blot (WB)||1/20 - 1/200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-85293 is specific for the beta subunit of Cholera toxin in bacterial samples.
This product has an Enzyme: Protein (Molar Ratio) of 2-3.
The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.