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Choline Transporter (SLC5A7) (extracellular) Polyclonal Antibody

View all (8) SLC5A7 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Choline Transporter (SLC5A7) (extracellular) Antibody in Immunocytochemistry (ICC/IF) — Expression of Choline transporter in rat PC12 cells - Cell surface detection of CHT in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (#ACT-001), (1:100), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of the two images.

Choline Transporter (SLC5A7) (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of Choline transporter in rat striatum - Immunohistochemical staining of rat frozen brain sections using Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (#ACT-001) (1:200). A. CHT expression (green)is detected in the matrix (M) but not in the patches (P).B. DAPI (blue) staining provides general cellular staining. C. merge of A and B shows that CHT appears in a subset of striatal neurons (arrows).

Choline Transporter (SLC5A7) (extracellular) Antibody in Western Blot (WB) — Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates: - 1-3. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (#ACT-001), (1:200).4-6. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody , preincubated with Choline Transporter/SLC5A7 (extracellular) Blocking Peptide (#BLP-CT001).

Product Details

ACT-001-200UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:200	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	(C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter, 4thextracellular loop View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.8 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Acetylcholine functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebates, and is the principle neurotransmitter used at the neuromuscular junction. Presynaptic synthesis of acetylcholine requires a steady supply of choline, which is acquired by a plasma membrane high affinity choline transporter (CHT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CHT; hCHT; Hemicholinium-3-sensitive choline transporter; High affinity choline transporter 1; high affinity choline transporter; hemicholinium-3-sensitive choline transporter; MGC126299; MGC126300; solute carrier family 5 (choline transporter), member 7; solute carrier family 5 (sodium/choline cotransporter), member 7; Solute carrier family 5 member 7; solute carrier family 5, member 7

Gene Aliases: CHT; CHT1; HMN7A; SLC5A7

UniProt ID: (Human) Q9GZV3, (Mouse) Q8BGY9, (Rat) Q9JMD7

Entrez Gene ID: (Human) 60482, (Mouse) 63993, (Rat) 85426

Molecular Function: transporter