|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Schizosaccharomyces pombe Chp1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||2.5 µg for 10^7 cells|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34611 targets Chp1 in ChIP and WB applications and shows reactivity with Yeast samples.
The PA5-34611 immunogen is synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Schizosaccharomyces pombe Chp1.
PA5-34611 detects Chp1 which has a predicted molecular weight of approximately 109 kDa.
Chp1 a chromodomain protein associates with the S. pombe mating type locus and telomeres. Chp1 localization to heterochromatin depends on its chromodomain and the C-terminal domain of the protein. Chp1 and centromeric siRNAs are components of the RITS (RNA-induced initiation of transcriptional gene silencing) complex. The association of RITS with centromeres is linked to Dicer activity, which is required to cleave centromeric transcripts to produce short interfering RNAs that actively recruit components of heterochromatin to centromeres.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.