Immunohistochemistry was performed on human pancreas tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, 70% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 8-15 min. Following antigen retrieval, tissues were blocked in a 3% H2O2-methanol solution for 15 minutes at room temperature. Tissue was then probed with a Chromogranin A Mouse Monoclonal antibody (Product # MA5-13281) at a dilution of 1:100 in 3% BSA in PBS overnight at 4°C in a humidified chamber. Negative control tissue received no primary antibody. Tissues were washed extensively with PBST, and detection was performed using a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB. Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 10 publications below|
MA5-13281 targets Chromogranin A in IF and IHC (P) applications and shows reactivity with Human and Non-human primate samples.
The MA5-13281 immunogen is human pheochromocytoma.
Chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bevacizumab plus octreotide and metronomic capecitabine in patients with metastatic well-to-moderately differentiated neuroendocrine tumors: the XELBEVOCT study.
MA5-13281 was used in immunohistochemistry to study the performance of the XELBEVOCT therapeutic regimen in patients with well-to-moderately differentiated neuroendocrine tumors
|Berruti A,Fazio N,Ferrero A,Brizzi MP,Volante M,Nobili E,Tozzi L,Bodei L,Torta M,D'Avolio A,Priola AM,Birocco N,Amoroso V,Biasco G,Papotti M,Dogliotti L||BMC cancer (14:null)||2014|
Human papillomavirus-associated adenocarcinoma of the base of the tongue.
MA5-13281 was used in immunohistochemistry to report on two cases of adenocarcinoma of the base of the tongue associated with HPV infection
|Hanna J,Reimann JD,Haddad RI,Krane JF||Human pathology (44:1516)||2013|
Tumor staging but not grading is associated with adverse clinical outcome in neuroendocrine tumors of the appendix: a retrospective clinical pathologic analysis of 138 cases.
MA5-13281 was used in immunohistochemistry to study the association of tumor staging with clinical outcome in neuroendocrine tumors of the appendix
|Volante M,Daniele L,Asioli S,Cassoni P,Comino A,Coverlizza S,De Giuli P,Fava C,Manini C,Berruti A,Papotti M||The American journal of surgical pathology (37:606)||2013|
Human achaete-scute homolog-1 expression in neuroendocrine breast carcinoma.
MA5-13281 was used in immunohistochemistry to study the expression of the hASH-1 transcription factor in neuroendocrine breast carcinoma
|Righi L,Rapa I,Votta A,Papotti M,Sapino A||Virchows Archiv : an international journal of pathology (460:415)||2012|
HPV-associated neuroendocrine carcinoma of the oropharynx: a rare new entity with potentially aggressive clinical behavior.
MA5-13281 was used in immunohistochemistry to report on 8 cases of HPV- and tobacco-associated neuroendocrine carcinoma of the oropharynx
|Kraft S,Faquin WC,Krane JF||The American journal of surgical pathology (36:321)||2012|
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
MA5-13281 was used in immunohistochemistry to study pulmonary meningothelial-like nodules
|Mukhopadhyay S,El-Zammar OA,Katzenstein AL||The American journal of surgical pathology (33:487)||2009|
Laparoscopic resection of urinary bladder paraganglioma: a case report.
MA5-13281 was used in immunohistochemistry to report on a case of aparoscopic resection of urinary bladder paraganglioma
|Dilbaz B,Bayoglu Y,Oral S,Cavusoglu D,Uluoglu O,Dilbaz S||Surgical laparoscopy, endoscopy and percutaneous techniques (16:58)||2006|
Pulmonary large cell carcinoma with rhabdoid phenotype.
MA5-13281 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype
|Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G||Annals of diagnostic pathology (9:223)||2005|
Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.
MA5-13281 was used in immunohistochemistry to study the altered distribution of metaplastic cell types in atrophic gastritic mucosa with endocrine cell lesions
|Deveci MS,Deveci G||The Tohoku journal of experimental medicine (202:13)||2004|
Classification of low-grade neuroendocrine tumors of midgut and unknown origin.
MA5-13281 was used in immunohistochemistry to study whether refining the grading of low-grade neuroendocrine tumors of the midgut and unknown origin is of prognostic/predictive value
|Van Eeden S,Quaedvlieg PF,Taal BG,Offerhaus GJ,Lamers CB,Van Velthuysen ML||Human pathology (33:1126)||2002|