Invitrogen

Chromogranin A Polyclonal Antibody

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Chromogranin A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Chromogranin A of paraffin-embedded Human colon cancer tissue using a Chromogranin-A Polyclonal antibody (Product #PA5-85952). Counter stained with hematoxylin.

Chromogranin A Antibody in Western Blot (WB) — Western blot analysis of Chromogranin A in PC12 cell lysate using a Chromogranin A Polyclonal antibody (Product # PA5-85952) at a dilution of 1:2000.

Chromogranin A Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of Chromogranin A in Lovo cells using a Chromogranin A Polyclonal Antibody (Product # PA5-85952) at a dilution of 1:100, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100-1:200

Immunohistochemistry (IHC)

1:200

Western Blot (WB)

1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to C-terminal of Mouse Chromogranin A

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 1% BSA, 40% Glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AL-11; AL26; Beta-granin; betagranin (N-terminal fragment of chromogranin A); Catestatin; CgA; chromofungin; Chromogranin-A; EA-92; ER-37; ES-43; GE-25; GR-44; GV-19; LF-19; p-Glu serpinin precursor; Pancreastatin; parathyroid secretory protein 1; Pituitary secretory protein I; Serpinin; Serpinin-RRG; SL21; SP-I; SS-18; Vasostatin I; Vasostatin II; Vasostatin-1; Vasostatin-2; WA-8; WE-14

Gene Aliases: CGA; CHGA; ChrA

UniProt ID: (Human) P10645, (Mouse) P26339

Entrez Gene ID: (Human) 1113, (Rat) 24258, (Mouse) 12652

Molecular Function: peptide hormone signaling molecule