Invitrogen

Chromogranin A Monoclonal Antibody (PHE5)

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Chromogranin A Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on human pancreas tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, 70% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 8-15 min. Following antigen retrieval, tissues were blocked in a 3% H2O2-methanol solution for 15 minutes at room temperature. Tissue was then probed with a Chromogranin A Mouse Monoclonal antibody (Product # MA5-13281) at a dilution of 1:100 in 3% BSA in PBS overnight at 4°C in a humidified chamber. Negative control tissue received no primary antibody. Tissues were washed extensively with PBST, and detection was performed using a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB. Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1.0 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 11 publications below

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

PHE5

Immunogen

Human pheochromocytoma

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10977528

Product Specific Information

MA5-13281 targets Chromogranin A in IF and IHC (P) applications and shows reactivity with Human and Non-human primate samples.

The MA5-13281 immunogen is human pheochromocytoma.

Target Information

Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AL-11; betagranin (N-terminal fragment of chromogranin A); Catestatin; CgA; chromofungin; Chromogranin-A; EA-92; ER-37; ES-43; GE-25; GR-44; GV-19; LF-19; p-Glu serpinin precursor; Pancreastatin; parathyroid secretory protein 1; Pituitary secretory protein I; Serpinin; Serpinin-RRG; SL21; SP-I; SS-18; Vasostatin I; Vasostatin II; Vasostatin-1; Vasostatin-2; WA-8; WE-14

Gene Aliases: CGA; CHGA

UniProt ID: (Human) P10645

Entrez Gene ID: (Human) 1113

Molecular Function: peptide hormone signaling molecule