Invitrogen

Chromogranin A Monoclonal Antibody (PHE5)

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Chromogranin A Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on human pancreas tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, 70% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 8-15 min. Following antigen retrieval, tissues were blocked in a 3% H2O2-methanol solution for 15 minutes at room temperature. Tissue was then probed with a Chromogranin A Mouse Monoclonal antibody (Product # MA5-13281) at a dilution of 1:100 in 3% BSA in PBS overnight at 4°C in a humidified chamber. Negative control tissue received no primary antibody. Tissues were washed extensively with PBST, and detection was performed using a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB. Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.

Product Details

MA5-13281

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 11 publications 11 publications
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1.0 µg/mL	-

Product Specifications
Species Reactivity	Human, Non-human primate
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	PHE5
Immunogen	Human pheochromocytoma
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10977528

Product Specific Information

MA5-13281 targets Chromogranin A in IF and IHC (P) applications and shows reactivity with Human and Non-human primate samples.

The MA5-13281 immunogen is human pheochromocytoma.

Target Information

Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bevacizumab plus octreotide and metronomic capecitabine in patients with metastatic well-to-moderately differentiated neuroendocrine tumors: the XELBEVOCT study.

BMC cancer

Berruti A,Fazio N,Ferrero A,Brizzi MP,Volante M,Nobili E,Tozzi L,Bodei L,Torta M,D'Avolio A,Priola AM,Birocco N,Amoroso V,Biasco G,Papotti M,Dogliotti L

MA5-13281 was used in immunohistochemistry to study the performance of the XELBEVOCT therapeutic regimen in patients with well-to-moderately differentiated neuroendocrine tumors

Fri Mar 14 00:00:00 EDT 2014

Human ASH-1 promotes neuroendocrine differentiation in androgen deprivation conditions and interferes with androgen responsiveness in prostate cancer cells.

The Prostate

Rapa I,Volante M,Migliore C,Farsetti A,Berruti A,Vittorio Scagliotti G,Giordano S,Papotti M

MA5-13281 was used in immunohistochemistry to study the role of the human ASH-1 transcription factor in the development of the prostate cancer cell neuroendocrine phenotype

Thu Aug 01 00:00:00 EDT 2013

Human papillomavirus-associated adenocarcinoma of the base of the tongue.

Human pathology

Hanna J,Reimann JD,Haddad RI,Krane JF

MA5-13281 was used in immunohistochemistry to report on two cases of adenocarcinoma of the base of the tongue associated with HPV infection

Thu Aug 01 00:00:00 EDT 2013

Tumor staging but not grading is associated with adverse clinical outcome in neuroendocrine tumors of the appendix: a retrospective clinical pathologic analysis of 138 cases.

The American journal of surgical pathology

Volante M,Daniele L,Asioli S,Cassoni P,Comino A,Coverlizza S,De Giuli P,Fava C,Manini C,Berruti A,Papotti M

MA5-13281 was used in immunohistochemistry to study the association of tumor staging with clinical outcome in neuroendocrine tumors of the appendix

Mon Apr 01 00:00:00 EDT 2013

Human achaete-scute homolog-1 expression in neuroendocrine breast carcinoma.

Virchows Archiv : an international journal of pathology

Righi L,Rapa I,Votta A,Papotti M,Sapino A

MA5-13281 was used in immunohistochemistry to study the expression of the hASH-1 transcription factor in neuroendocrine breast carcinoma

Sun Apr 01 00:00:00 EDT 2012

HPV-associated neuroendocrine carcinoma of the oropharynx: a rare new entity with potentially aggressive clinical behavior.

The American journal of surgical pathology

Kraft S,Faquin WC,Krane JF

MA5-13281 was used in immunohistochemistry to report on 8 cases of HPV- and tobacco-associated neuroendocrine carcinoma of the oropharynx

Thu Mar 01 00:00:00 EST 2012

Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.

The American journal of surgical pathology

Mukhopadhyay S,El-Zammar OA,Katzenstein AL

MA5-13281 was used in immunohistochemistry to study pulmonary meningothelial-like nodules

Wed Apr 01 00:00:00 EDT 2009

Laparoscopic resection of urinary bladder paraganglioma: a case report.

Surgical laparoscopy, endoscopy & percutaneous techniques

Dilbaz B,Bayoglu Y,Oral S,Cavusoglu D,Uluoglu O,Dilbaz S

MA5-13281 was used in immunohistochemistry to report on a case of aparoscopic resection of urinary bladder paraganglioma

Wed Feb 01 00:00:00 EST 2006

Pulmonary large cell carcinoma with rhabdoid phenotype.

Annals of diagnostic pathology

Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G

MA5-13281 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype

Mon Aug 01 00:00:00 EDT 2005

Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.

The Tohoku journal of experimental medicine

Deveci MS,Deveci G

MA5-13281 was used in immunohistochemistry to study the altered distribution of metaplastic cell types in atrophic gastritic mucosa with endocrine cell lesions

Thu Jan 01 00:00:00 EST 2004

Classification of low-grade neuroendocrine tumors of midgut and unknown origin.

Human pathology

Van Eeden S,Quaedvlieg PF,Taal BG,Offerhaus GJ,Lamers CB,Van Velthuysen ML

MA5-13281 was used in immunohistochemistry to study whether refining the grading of low-grade neuroendocrine tumors of the midgut and unknown origin is of prognostic/predictive value

Fri Nov 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: AL-11; betagranin (N-terminal fragment of chromogranin A); Catestatin; CgA; chromofungin; Chromogranin-A; CTNNB; DKFZp686D02253; EA-92; ER-37; ES-43; FLJ25606; FLJ37923; GE-25; GR-44; GV-19; LF-19; MGC105435; p-Glu serpinin precursor; Pancreastatin; parathyroid secretory protein 1; Pituitary secretory protein I; Serpinin; Serpinin-RRG; SL21; SP-I; SS-18; Vasostatin I; Vasostatin II; Vasostatin-1; Vasostatin-2; WA-8; WE-14

Gene Aliases: CGA; CHGA

UniProt ID: (Human) P10645

Entrez Gene ID: (Human) 1113

Function(s)