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Invitrogen
Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385)
FIGURE: 1 / 7
Chromogranin A Antibody (MA5-33052) in ICC/IF
Immunofluorescence analysis of Chga was performed using 70% confluent log phase SH-SY5Y treated with PTI (1X for 4hrs) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385) (Product # MA5-33052) ... View More
Product Details
MA5-33052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM385
Immunogen
A peptide corresponding to the N-terminus of human Chromogranin A.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810145
Product Specific Information
Specificity: reacts to Human Chromogranin A.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: betagranin (N-terminal fragment of chromogranin A); catestatin; CgA; chromofungin; Chromogranin-A; CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; MGC105435; parathyroid secretory protein 1; Pituitary secretory protein I; SP-I
Gene Aliases: CGA; CHGA
UniProt ID: (Human) Q9BQB5
Entrez Gene ID: (Human) 1113
regulation of the force of heart contraction
mast cell chemotaxis
organelle organization
regulation of blood pressure
positive regulation of cAMP metabolic process
killing of cells of other organism
mast cell cytokine production
negative regulation of catecholamine secretion
ovulation cycle
mast cell degranulation
innate immune response
mast cell activation
negative regulation of vasodilation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive regulation of cardiac muscle contraction
adrenergic receptor signaling pathway involved in cardiac muscle relaxation
positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway
negative regulation of neuron death
positive regulation of relaxation of cardiac muscle
positive regulation of dense core granule biogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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