Invitrogen

Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (108) Chromogranin A antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Chromogranin A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Chga was performed using 70% confluent log phase SH-SY5Y treated with PTI (1X for 4hrs) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385) (Product # MA5-33052) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasm localization. Panel e represents HepG2 cells treated with PTI showing low expression of Chga. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Chromogranin A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Chromogranin A in paraffin-embedded human colon cancer tissue section. Samples were incubated with Chromogranin A monoclonal antibody (Product # MA5-33052) using a 1:10,000 dilution.

Chromogranin A Antibody in Western Blot (WB) — Western blot was performed using Anti-Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385) (Product # MA5-33052) and a 65 kDa band corresponding to Chga was observed across cell lines along with an uncharacterized band (*). Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with PTI (1X for 4hrs) (Lane 2), Hep G2 (Lane 3), and Hep G2 treated with PTI (1X for 4hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Chromogranin A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-Chromogranin A Recombinant Rabbit Monoclonal Antibody (RM385) (Product # MA5-33052), shows expression in SH-SY5Y with protein transport inhibitor but not in HepG2 with protein transport inhibitor.

Product Details

MA5-33052

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:5,000-1:10,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM385

Immunogen

A peptide corresponding to the N-terminus of human Chromogranin A.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

RRID

AB_2810145

Product Specific Information

Specificity: reacts to Human Chromogranin A.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AL-11; betagranin (N-terminal fragment of chromogranin A); Catestatin; CgA; chromofungin; Chromogranin-A; CTNNB; DKFZp686D02253; EA-92; ER-37; ES-43; FLJ25606; FLJ37923; GE-25; GR-44; GV-19; LF-19; MGC105435; p-Glu serpinin precursor; Pancreastatin; parathyroid secretory protein 1; Pituitary secretory protein I; Serpinin; Serpinin-RRG; SL21; SP-I; SS-18; Vasostatin I; Vasostatin II; Vasostatin-1; Vasostatin-2; WA-8; WE-14

Gene Aliases: CGA; CHGA

UniProt ID: (Human) P10645

Entrez Gene ID: (Human) 1113

Function(s)

regulation of the force of heart contraction mast cell chemotaxis organelle organization regulation of blood pressure positive regulation of cAMP metabolic process killing of cells of other organism mast cell cytokine production negative regulation of catecholamine secretion ovulation cycle mast cell degranulation innate immune response mast cell activation negative regulation of vasodilation defense response to Gram-negative bacterium defense response to Gram-positive bacterium defense response to fungus positive regulation of cardiac muscle contraction adrenergic receptor signaling pathway involved in cardiac muscle relaxation positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway negative regulation of neuron death positive regulation of relaxation of cardiac muscle positive regulation of dense core granule biogenesis