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Invitrogen
Citrulline Monoclonal Antibody (2D3.1)
FIGURE: 1 / 3
Citrulline Antibody (MA5-27573) in ICC/IF
Immunofluorescent analysis of Citrulline in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Citrulline monoclonal antibody (Product # MA5-27573) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min at RT). I... View More
Product Details
MA5-27573
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2D3.1
Immunogen
Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735148
Product Specific Information
A 1:1,000 dilution of MA5-27573 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid that is not incorporated into proteins during synthesis but is generated within proteins by the deimination of arginine. Citrulline occurs normally in many proteins, such as Myelin basic protein (MBP) while others, such as fibrin become citrullinated during inflammation or apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACPA; Citrulline; L-Citrulline
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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