|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgM|
|Storage buffer||TBS, pH 8.0|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2-5 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Host erythrocyte environment influences the localization of exported protein 2, an essential component of the Plasmodium translocon.
MA1-10316 was used in immunocytochemistry to examine exported protein 2 localization in Plasmodium vivax-infected reticulocytes.
|Meibalan E,Comunale MA,Lopez AM,Bergman LW,Mehta A,Vaidya AB,Burns JM||Eukaryotic cell (14:371)||2015|