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Immunofluorescence analysis of Claudin-1 was done on 90% confluent log phase CaCo-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Claudin-1 Mouse Monoclonal Antibody (374900) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization of Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059). and panel e is a no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human and mouse claudin-1 proteins|
|Storage buffer||PBS, pH 7.4, with 4.5mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 µg/ml|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 µg/ml|
|Immunoprecipitation (IP)||10 µg/test|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.
Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selection of a hepatitis C virus with altered entry factor requirements reveals a genetic interaction between the E1 glycoprotein and claudins.
37-4900 was used in western blot to examine genetic interactions between claudin-1 and hepatitis C virus
|Hopcraft SE,Evans MJ||Hepatology (Baltimore, Md.) (62:1059)||2015|
Biophysical characterization of interactions between the C-termini of peripheral nerve claudins and the PDZ₁ domain of zonula occludens.
37-4900 was used in western blot to investigate the specific roles of the 3 C-terminal claudin residues by assessing their interactions with PDZ1 of ZO2.
|Wu J,Peng D,Zhang Y,Lu Z,Voehler M,Sanders CR,Li J||Biochemical and biophysical research communications (459:87)||2015|
2,4,6-trinitrobenzene sulfonic acid-induced chronic colitis with fibrosis and modulation of TGF-β1 signaling.
37-4900 was used in western blot to evalute the therapeutic potential of proteasome inhibition for intestinal fibrosis in rats.
|Loeuillard E,Bertrand J,Herranen A,Melchior C,Guérin C,Coëffier M,Aziz M,Déchelotte P,Savoye G,Marion-Letellier R||World journal of gastroenterology (20:18207)||2014|
Alteration of intestinal barrier function during activity-based anorexia in mice.
37-4900 was used in western blot to study the effect of activity-based anorexia on intestinal barrier function in mice
|Jésus P,Ouelaa W,François M,Riachy L,Guérin C,Aziz M,Do Rego JC,Déchelotte P,Fetissov SO,Coëffier M||Clinical nutrition (Edinburgh, Scotland) (33:1046)||2014|
Expression of myoferlin in human airway epithelium and its role in cell adhesion and zonula occludens-1 expression.
37-4900 was used in western blot to study the function of dysferlin and myoferlin in airway epithelial homeostasis.
|Leung C,Shaheen F,Bernatchez P,Hackett TL||PloS one (7:null)||2012|
|Dynamics of claudins expression in colitis and colitis-associated cancer in rat.||Arimura Y,Nagaishi K,Hosokawa M||Methods in molecular biology (Clifton, N.J.) (762:409)||2011|
|Human||Not Cited||Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.||Jacob C,Yang PC,Darmoul D,Amadesi S,Saito T,Cottrell GS,Coelho AM,Singh P,Grady EF,Perdue M,Bunnett NW||The Journal of biological chemistry (280:31936)||2005|
Contrasting roles of mitogen-activated protein kinases in cellular entry and replication of hepatitis C virus: MKNK1 facilitates cell entry.
37-4900 was used in western blot to study the roles of MAPK-related kinase and MKNK-1 in HCV replication and cellular entry.
|Kim S,Ishida H,Yamane D,Yi M,Swinney DC,Foung S,Lemon SM||Journal of virology (87:4214)||2013|
Evidence for phenotypic plasticity in aggressive triple-negative breast cancer: human biology is recapitulated by a novel model system.
37-4900 was used in western blot to use a novel cell-based model system to study phenotypic plasticity in triple-negative breast cancer.
|D'Amato NC,Ostrander JH,Bowie ML,Sistrunk C,Borowsky A,Cardiff RD,Bell K,Young LJ,Simin K,Bachelder RE,Delrow J,Dawson A,Yee LD,Mrózek K,Clay TM,Osada T,Seewaldt VL||PloS one (7:null)||2012|
Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction.
37-4900 was used in immunocytochemistry to examine the functions of symplekin at tight junctions.
|Chang H,Zhang C,Cao Y||Histochemistry and cell biology (137:319)||2012|
Impact of intra- and interspecies variation of occludin on its function as coreceptor for authentic hepatitis C virus particles.
37-4900 was used in western blot to investigate how the differences between human and murine occludin contribute to hepatitis C virus spread.
|Ciesek S,Westhaus S,Wicht M,Wappler I,Henschen S,Sarrazin C,Hamdi N,Abdelaziz AI,Strassburg CP,Wedemeyer H,Manns MP,Pietschmann T,von Hahn T||Journal of virology (85:7613)||2011|
Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.
37-4900 was used in immunocytochemistry and western blot to develop and characterize a model for Hailey-Hailey disease using normal keratinocytes.
|Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
Loss of claudin-1 in lipopolysaccharide-treated periodontal epithelium.
37-4900 was used in immunohistochemistry - paraffin section to study the effects of lipopolysaccharide on the expression of tight junction-associated molecule genes in the junctional epithelium.
|Fujita T,Firth JD,Kittaka M,Ekuni D,Kurihara H,Putnins EE||Journal of periodontal research (47:222)||2012|
|Mouse||1:500||Differential distribution of tight junction proteins suggests a role for tanycytes in blood-hypothalamus barrier regulation in the adult mouse brain.||Mullier A,Bouret SG,Prevot V,Dehouck B||The Journal of comparative neurology (518:943)||2010|
||Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.||Jacob C,Yang PC,Darmoul D,Amadesi S,Saito T,Cottrell GS,Coelho AM,Singh P,Grady EF,Perdue M,Bunnett NW||The Journal of biological chemistry (280:31936)||2005|
|Human||Not Cited||Loss of claudins-1 and -7 and expression of claudins-3 and -4 correlate with prognostic variables in prostatic adenocarcinomas.||Sheehan GM,Kallakury BV,Sheehan CE,Fisher HA,Kaufman RP,Ross JS||Human pathology (38:564)||2007|
claudin-1; CLD1; ILVASC; SEMP1; senescence-associated epithelial membrane protein 1
AI596271; CLD1; CLDN1; ILVASC; SEMP1; UNQ481/PRO944