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Invitrogen
Claudin 1 Polyclonal Antibody
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FIGURE: 1 / 7
Claudin 1 Antibody (PA1-37465) in WB
Western blot was performed using Anti-CLDN1 Polyclonal Antibody (Product # PA1-37465) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon EGF, LiCl treatment and IL-beta treatment. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), MDCK (Lane 2), A-431 (Lane 3), HEK-293 (Lane 4), Caco-2 treated with EGF (100 ng/mL) and LiCl (50mM) simultaneously for 24 Hours (Lane 5), Caco-2 treated with IL-beta (10 ng/mL for 48 Hours) (Lane 6), Caco-2 (Lane 7), tissue extracts (30 µg lysate) of Mous... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-37465
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from C-terminus of human claudin-1 peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. not determined
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, breast carcinoma or ovarian carcinoma.
Target Information
Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CLDN1; senescence-associated epithelial membrane protein 1; tight junction transmembrane protein; unnamed protein product
Gene Aliases: CLD1; ILVASC; SEMP1
UniProt ID: (Human) O95832, (Mouse) O88551
Entrez Gene ID: (Human) 9076, (Dog) 608207, (Mouse) 12737
establishment of blood-nerve barrier
response to toxic substance
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
positive regulation of cell migration
response to lipopolysaccharide
cell junction maintenance
maintenance of blood-brain barrier
cell-cell junction organization
response to ethanol
viral entry into host cell
protein oligomerization
positive regulation of epithelial cell proliferation involved in wound healing
establishment of skin barrier
xenobiotic transport across blood-nerve barrier
response to interleukin-18
bicellular tight junction assembly
cellular response to lead ion
cellular response to interferon-gamma
cellular response to tumor necrosis factor
response to dexamethasone
cellular response to transforming growth factor beta stimulus
positive regulation of wound healing
establishment of endothelial intestinal barrier
liver regeneration
positive regulation of bicellular tight junction assembly
cellular response to butyrate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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