Immunofluorescence analysis of Claudin 1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Claudin 1 Rabbit Polyclonal Antibody (PA516833) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminus of human Claudin-1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16833 targets Claudin 1 in IHC (P) and WB applications and shows reactivity with Canine, Human, mouse, Non-human primate, and Rat samples.
The PA5-16833 immunogen is a synthetic peptide derived from the C-terminus of human Claudin-1.
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Renin lineage cells repopulate the glomerular mesangium after injury.
PA5-16833 was used in immunohistochemistry - frozen section to assess repopulation of glomerular mesangium after injury by renin lineage cells
|Starke C,Betz H,Hickmann L,Lachmann P,Neubauer B,Kopp JB,Sequeira-Lopez ML,Gomez RA,Hohenstein B,Todorov VT,Hugo CP||Journal of the American Society of Nephrology : JASN (26:48)||2015|
Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation.
PA5-16833 was used in immunohistochemistry - paraffin section to determine the role of Notch signaling in the development of the anterior pigmented epithelium
|Sarode B,Nowell CS,Ihm J,Kostic C,Arsenijevic Y,Moulin AP,Schorderet DF,Beermann F,Radtke F||Pigment cell and melanoma research (27:580)||2014|
Noninvasive delivery of siRNA and plasmid DNA into skin by fractional ablation: erbium:YAG laser versus CO¿ laser.
PA5-16833 was used in immunohistochemistry to compare the relative efficacies of erbium-YAG lasers and CO2 lasers for the delivery of siRNA and plasmid DNA via fractional laser-mediated skin ablation
|Lee WR,Shen SC,Chen WY,Aljuffali IA,Suen SY,Fang JY||European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (86:315)||2014|
Risk assessment of excess drug and sunscreen absorption via skin with ablative fractional laser resurfacing : optimization of the applied dose for postoperative care.
PA5-16833 was used in immunohistochemistry to study the absorption of drugs and sunscreen through the skin following ablative fractional laser resurfacing
|Chen WY,Fang CL,Al-Suwayeh SA,Yang HH,Li YC,Fang JY||Lasers in medical science (28:1363)||2013|
An insight into the role of barrier related skin proteins.
PA5-16833 was used in immunohistochemistry to study the effect of penetration enhancers on barrier-related proteins in skin
|Wato K,Hara T,Yamana K,Nakao H,Inagi T,Terada K||International journal of pharmaceutics (427:293)||2012|
Claudin-1 expression in squamous cell carcinomas of different organs: comparative study of cancerous tissues and normal controls.
PA5-16833 was used in immunohistochemistry to study claudin-1 expression in squamous cell carcinomas of different organs
|Ouban A,Hamdan H,Hakam A,Ahmed AA||International journal of surgical pathology (20:132)||2012|
Indigo naturalis upregulates claudin-1 expression in human keratinocytes and psoriatic lesions.
PA5-16833 was used in immunocytochemistry, immunohistochemistry, and western blot to study the elevation of claudin-1 expression in human keratinocytes and psoriatic lesions following treatment with extracts of indigo naturalis
|Lin YK,Chen HW,Leu YL,Yang YL,Fang Y,Su Pang JH,Hwang TL||Journal of ethnopharmacology (145:614)||2013|