Immunofluorescence analysis of Claudin-18 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ Claudin-18 Recombinant Rabbit Monoclonal Antibody (700178) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel c is a merged image showing cell junction localization and panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 224-237 of P56856.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear. Because of its highly restricted expression pattern in normal tissues and frequent activation in numerous human cancers, claudin-18 may represent an important target for antibody therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.
700178 was used in immunohistochemistry - paraffin section to study inhibition of initiation of pancreatic ductal adenocarcinoma due to acinar differentiation determinant PTF1A
|Krah NM,De La O JP,Swift GH,Hoang CQ,Willet SG,Chen Pan F,Cash GM,Bronner MP,Wright CV,MacDonald RJ,Murtaugh LC||eLife (4:null)||2015|
Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.
700178 was used in immunohistochemistry to report on a case of ovarian mucinous cystadenoma
|Dainese E,Cimetti L,Capella C,Riva C||Pathology, research and practice (209:670)||2013|
The relative balance of GM-CSF and TGF-ß1 regulates lung epithelial barrier function.
700178 was used in western blot to test if the relative balance of GM-CSF and TGF-β signaling regulates lung epithelial barrier function.
|Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M||American journal of physiology. Lung cellular and molecular physiology (308:L1212)||2015|
NF-¿B inhibitors impair lung epithelial tight junctions in the absence of inflammation.
700178 was used in immunohistochemistry to investigate the effect of NF-kappaB inhibitors on lung epithelial tight junctions in the absence of inflammation
|Ward C,Schlingmann BL,Stecenko AA,Guidot DM,Koval M||Tissue barriers (3:null)||2015|
Primary gastric Merkel cell carcinoma harboring DNA polyomavirus: first description of an unusual high-grade neuroendocrine carcinoma.
700178 was used in immunohistochemistry to reoprt on a case of an unusual primary gastric Merkel cell carcinoma
|Capella C,Marando A,Longhi E,Bernasconi B,Finzi G,Parravicini C,Sessa F,La Rosa S||Human pathology (45:1310)||2014|
Distribution of LGR5+ cells and associated implications during the early stage of gastric tumorigenesis.
700178 was used in immunohistochemistry to study the potential cancer stem cell role of LGR5+ cells in gastric tumorigenesis
|Jang BG,Lee BL,Kim WH||PloS one (8:null)||2013|