Invitrogen

Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18 Published Figures

27 References

View all (7) Claudin 18 antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Claudin 18 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Claudin-18 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image showing cell junction localization and panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.

Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Claudin-18 was performed on paraffin-embedded human normal stomach and squamous lung carcinoma tissue sections. To expose target proteins, endogenous peroxide blocking followed by heat induced epitope retrieval was performed using Tris-EDTA (pH 9. 0) buffer for 15 minutes. Following antigen retrieval, tissues were blocked in 0. 2% BSA with 0. 1% cold fish skin gelatin in 1X PBS for 1 hour in a humidified chamber. The tissues were then probed with a Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a dilution of 2 µg/mL for 3 hours at room temperature in a humidified chamber. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody (Product # G-21234), followed by colorimetric detection using DAB. Images were taken at 40x (normal stomach) or 20x (lung carcinoma) magnification. Note membrane staining of normal stomach and cytoplasmic staining of lung carcinoma.

Claudin 18 Antibody in Western Blot (WB) — Western blot analysis of Claudin-18 was performed by loading 30 µg of rat Heart, A431 and A549 lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Claudin-18 was detected at ~27 kDa using Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a 1:1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody (Product # G-21234) at a 1:5000 dilution and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Claudin 18 Antibody in Immunohistochemistry (IHC) — Relative expression of claudins 3 and 18 in wild-type, cryptorchid, and orchiopexied dog testes. ( A ) Vimentin and claudin-3. ( B ) Vimentin and claudin-18 were detected in wild-type, cryptorchid, and orchiopexied dog testes by immunostaining analysis. Vimentin, claudin-3, and claudin-18 were localized in the cell membrane near the basement membrane of the testes. Scale bars = 50 mum; n = 3, two pairs of testes.

Claudin 18 Antibody — Figure 4. BMS-345541 destabilized tight junction associated claudin-18. Primary rat alveolar epithelial cells were isolated and cultured on Transwell permeable supports for 5 d to produce model type I cell monolayers. The cells were then incubated overnight with vehicle control ( A , C ) or 4 muM BMS-345541( B , D ), fixed and processed to image claudin-18 by immunofluorescence microscopy. Magnified insets are shown in C , D . In contrast to control cells, where claudin-18 was strongly localized to tight junctions at the plasma membrane, cells treated with BMS-345541 showed linear trails emanating from the plasma membrane (tight junction spikes; arrowheads). Bar - 10 micron.

Product Details

700178

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Dog, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Expression System

EXPI293

Class

Recombinant Monoclonal

Type

Antibody

Clone

34H14L15

Immunogen

A peptide corresponding to amino acids 224-237 of P56856.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532290

Product Specific Information

This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptomic Analysis of Testicular Gene Expression in a Dog Model of Experimentally Induced Cryptorchidism.

Cells

Jhun H,Lee WY,Park JK,Hwang SG,Park HJ

700178 was used in Immunocytochemistry-immunofluorescence to indicate that testicular hyperthermia caused by cryptorchidism affects claudin expression, regulated germ cell death, and the proliferation of Sertoli cells.

Wed Aug 10 00:00:00 EDT 2022

Transcriptomic Analysis of Testicular Gene Expression in a Dog Model of Experimentally Induced Cryptorchidism.

Cells

Jhun H,Lee WY,Park JK,Hwang SG,Park HJ

Wed Aug 10 00:00:00 EDT 2022

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

700178 was used in Western Blot, Immunohistochemistry to establish oocytes as an expression and two-cell screening model for tight junction integrity analysis of organ- and tissue-specific claudins by the characterization of homo- and heterophilic trans-interaction dependent on barrier effectors.

Sat Jul 23 00:00:00 EDT 2022

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

Sat Jul 23 00:00:00 EDT 2022

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

Sat Jul 23 00:00:00 EDT 2022

Asymmetric distribution of dynamin-2 and β-catenin relative to tight junction spikes in alveolar epithelial cells.

Tissue barriers

Lynn KS,Easley KF,Martinez FJ,Reed RC,Schlingmann B,Koval M

700178 was used in Immunocytochemistry-immunofluorescence to suggest a novel role for dynamin-2 in tight junction spike formation by reorienting junction-associated actin.

Sat Jul 03 00:00:00 EDT 2021

Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer.

Journal of gastric cancer

Kim SR,Shin K,Park JM,Lee HH,Song KY,Lee SH,Kim B,Kim SY,Seo J,Kim JO,Roh SY,Kim IH

700178 was used in Immunohistochemistry-immunofluorescence to investigate the clinical significance of CLDN18.2 and other cell-adherens junction molecules (Rho GTPase-activating protein [RhoGAP] and E-cadherin) in metastatic diffuse-type gastric cancer (mDGC).

Tue Dec 01 00:00:00 EST 2020

Nuclear staining of claudin-18 is a new immunohistochemical marker for diagnosing intramucosal well-differentiated gastric adenocarcinoma.

Pathology international

Takahashi Y,Tsutsumi Y,Takeuchi C,Shiogama K,Mizutani Y,Inada KI,Yamamichi N,Koike K

700178 was used in Immunohistochemistry to evaluate the association between histopathology and immunohistochemical expression of MUC1, HER2, p53, CEA, E-cadherin, β-catenin and claudin-18.

Tue Sep 01 00:00:00 EDT 2020

Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.

Cellular and molecular gastroenterology and hepatology

Quilichini E,Fabre M,Dirami T,Stedman A,De Vas M,Ozguc O,Pasek RC,Cereghini S,Morillon L,Guerra C,Couvelard A,Gannon M,Haumaitre C

700178 was used in Immunohistochemistry to reveal that Hnf1b deficiency may contribute to diseases of the exocrine pancreas and could gain further insight into the etiology of pancreatitis and tumorigenesis.

Mon Jul 20 00:00:00 EDT 2020

Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.

Scientific reports

Lee-Thacker S,Jeon H,Choi Y,Taniuchi I,Takarada T,Yoneda Y,Ko C,Jo M

700178 was used in Immunohistochemistry to provide in vivo evidence that CBFs act as essential transcriptional regulators of both ovulation and luteinization by regulating the expression of key genes that are involved in inflammation, matrix remodeling, cell differentiation, vascularization, and steroid metabolisms in mice.

Thu Jun 18 00:00:00 EDT 2020

Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

PloS one

Weber B,Mendler MR,Lackner I,von Zelewski A,Höfler S,Baur M,Braun CK,Hummler H,Schwarz S,Pressmar J,Kalbitz M

700178 was used in Immunocytochemistry-immunoflourescence to study early morphological, immunological and structural alterations in lung tissue after asphyxia and hemorrhage.

Fri Feb 21 00:00:00 EST 2020

Prevention and Reversion of Pancreatic Tumorigenesis through a Differentiation-Based Mechanism.

Developmental cell

Krah NM,Narayanan SM,Yugawa DE,Straley JA,Wright CVE,MacDonald RJ,Murtaugh LC

700178 was used in Immunohistochemistry to suggest that reactivation of an endogenous differentiation program can prevent and reverse oncogene-driven transformation in cells harboring tumor-driving mutations, introducing a potential paradigm for solid tumor prevention and treatment.

Mon Sep 23 00:00:00 EDT 2019

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Tissue barriers

Chen H,Lu R,Zhang YG,Sun J

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Mon Apr 01 00:00:00 EDT 2019

CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro.

International journal of cancer

Luo J,Chimge NO,Zhou B,Flodby P,Castaldi A,Firth AL,Liu Y,Wang H,Yang C,Marconett CN,Crandall ED,Offringa IA,Frenkel B,Borok Z

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Sat Dec 15 00:00:00 EST 2018

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

Zhou B,Flodby P,Luo J,Castillo DR,Liu Y,Yu FX,McConnell A,Varghese B,Li G,Chimge NO,Sunohara M,Koss MN,Elatre W,Conti P,Liebler JM,Yang C,Marconett CN,Laird-Offringa IA,Minoo P,Guan K,Stripp BR,Crandall ED,Borok Z

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.

eLife

Krah NM,De La O JP,Swift GH,Hoang CQ,Willet SG,Chen Pan F,Cash GM,Bronner MP,Wright CV,MacDonald RJ,Murtaugh LC