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Immunohistochemistry analysis of Claudin 2 showing staining in the cytoplasm and membrane of paraffin-embedded human hepatocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 2 monoclonal antibody (325600) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Non-human primate, Mouse, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of mouse Claudin-2|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-3 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 8 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
Claudin-2 belongs to the claudin family of integral membrane proteins. claudins are responsible for the formation of tight junction strands, and are connected to the actin cytoskeleton through ZO-1. Claudin-2 functions as a paracellular channel with cation (Na+) selectivity at tight junctions. The expression of claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways.
32-5600 was used in immunocytochemistry to study the modulatory effect of TLR4/EGF signaling pathways on TPN-associated intestinal epithelial cell atrophy.
|Freeman JJ,Feng Y,Demehri FR,Dempsey PJ,Teitelbaum DH||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:2943)||2015|
Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells.
32-5600 was used in immunocytochemistry and western blot to characterize TALEN-mediated claudin-2 knockout in MDCK cells
|Tokuda S,Furuse M||PloS one (10:null)||2015|
Oxidative stress induced by potassium bromate exposure results in altered tight junction protein expression in renal proximal tubule cells.
32-5600 was used in immunocytochemistry and western blot to determine the effects of KBrO(3) using human and rat proximal tubular cell lines.
|Limonciel A,Wilmes A,Aschauer L,Radford R,Bloch KM,McMorrow T,Pfaller W,van Delft JH,Slattery C,Ryan MP,Lock EA,Jennings P||Archives of toxicology (86:1741)||2012|
Changes in Epithelial Barrier Function in Response to Parasitic Infection: Implications for IBD Pathogenesis.
32-5600 was used in immunohistochemistry to examine the mast cell proteinase and tight junction protein kinetics in a rat model of post-infectious gut dysfunction.
|Fernández-Blanco JA,Estévez J,Shea-Donohue T,Martínez V,Vergara P||Journal of Crohn's & colitis (9:463)||2015|
|Human||1:800||Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.||Collins JE,Kirk A,Campbell SK,Mason J,Wilson SJ||Methods in molecular biology (Clifton, N.J.) (762:371)||2011|
|Human||Not Cited||Differential expression of claudin tight junction proteins in the human cortical nephron.||Kirk A,Campbell S,Bass P,Mason J,Collins J||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (25:2107)||2010|
(-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity.
32-5600 was used in western blot to study the efficacy of (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity.
|Contreras TC,Ricciardi E,Cremonini E,Oteiza PI||Archives of biochemistry and biophysics (573:84)||2015|
POF1B localizes to desmosomes and regulates cell adhesion in human intestinal and keratinocyte cell lines.
32-5600 was used in western blot to investigate the localization of POF1B in desmosomes and its role in human intestinal and keratinocyte cell lines
|Crespi A,Bertoni A,Ferrari I,Padovano V,Della Mina P,Berti E,Villa A,Pietrini G||The Journal of investigative dermatology (135:192)||2015|
Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel.
32-5600 was used in immunocytochemistry and western blot to evaluate claudin 2 as a proximal tubular stress responsive paracellular water channel
|Wilmes A,Aschauer L,Limonciel A,Pfaller W,Jennings P||Toxicology and applied pharmacology (279:163)||2014|
ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.
32-5600 was used in immunocytochemistry and western blot to characterize the TALEN-mediated ZO-1 knockout in MDCK cells
|Tokuda S,Higashi T,Furuse M||PloS one (9:null)||2014|
Mutations in TJP2 cause progressive cholestatic liver disease.
32-5600 was used in immunohistochemistry and western blot to study the involvement of TJP2 mautations in progressive cholestatic liver disease
|Sambrotta M,Strautnieks S,Papouli E,Rushton P,Clark BE,Parry DA,Logan CV,Newbury LJ,Kamath BM,Ling S,Grammatikopoulos T,Wagner BE,Magee JC,Sokol RJ,Mieli-Vergani G,Smith JD,Johnson CA,McClean P,Simpson MA,Knisely AS,Bull LN,Thompson RJ||Nature genetics (46:326)||2014|
Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins.
32-5600 was used in western blot to assess the role of tight junctions and gap proteins in cervical remodeling.
|Ghulé VV,Gray C,Galimberti A,Anumba DO||Journal of translational medicine (10:null)||2012|
Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly.
32-5600 was used in western blot to analyze the role of paracingulin domains.
|Paschoud S,Guillemot L,Citi S||The Journal of biological chemistry (287:13159)||2012|
|Mouse||1:4000||Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture.||Colegio OR,Van Itallie C,Rahner C,Anderson JM||American journal of physiology. Cell physiology (284:C1346)||2003|
Innate immune factors in the development and maintenance of pouchitis.
32-5600 was used in flow cytometry to perform longitudinal and cross-sectional studies of patients after restorative proctocolectomy and assess tight junction proteins and dendritic cell characteristics in the ileal pouch
|Landy J,Al-Hassi HO,Ronde E,English NR,Mann ER,Bernardo D,Ciclitira PJ,Clark SK,Knight SC,Hart AL||Inflammatory bowel diseases (20:1942)||2014|
|Human||1:50||Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin.||Hintsala HR,Siponen M,Haapasaari KM,Karihtala P,Soini Y||International journal of clinical and experimental pathology (6:2855)||2013|
EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
32-5600 was used in immunohistochemistry - frozen section, immunoprecipitation, and western blot to report that mice lacking EpCAM have intestinal barrier defects and die shortly after birth as a result of intestinal erosion.
|Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H||Developmental biology (371:136)||2012|
|Dog||Not Cited||Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells.||Dukes JD,Fish L,Richardson JD,Blaikley E,Burns S,Caunt CJ,Chalmers AD,Whitley P||Molecular biology of the cell (22:3192)||2011|
claudin-2; CLD2; CLDN2; integral membrane protein claudin-2; SP82
AL022813; CLDN2; PSEC0059; SP82; UNQ705/PRO1356