Immunohistochemistry analysis of Claudin 3 showing staining in the membrane of paraffin-embedded human small intestine tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 3 Rabbit Polyclonal Antibody (PA516867) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Mouse, Rat, Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminus of mouse Claudin-3|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
PA5-16867 targets Claudin 3 in IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16867 immunogen is a synthetic peptide derived from the C-terminus of mouse Claudin-3.
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proximal tubular cells contain a phenotypically distinct, scattered cell population involved in tubular regeneration.
PA5-16867 was used in immunohistochemistry to study the role of sub-population of CD24(-)/CD133(-)/vimentin(+) proximal tubular cells in the regeneration of tubules
|Smeets B,Boor P,Dijkman H,Sharma SV,Jirak P,Mooren F,Berger K,Bornemann J,Gelman IH,Floege J,van der Vlag J,Wetzels JF,Moeller MJ||The Journal of pathology (229:645)||2013|
Integrin Beta 1 suppresses multilayering of a simple epithelium.
PA5-16867 was used in immunohistochemistry to study the conversion of simple epithelium into multilayer epithelium following ablation of integrin beta1 in a murine model
|Chen J,Krasnow MA||PloS one (7:null)||2013|