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Immunofluorescence analysis of Claudin-4 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Claudin-4 (3E2C1) Mouse Monoclonal Antibody Alexa Fluor® 594 conjugate (329494) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature. Cytoskeleton was stained with alpha-tubulin Mouse Monoclonal Antibody (Product# 322500, 1ug/ml) followed by Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
claudin-4; CLAUDIN4; CLDN4; clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; CPER; CPETR; CPETR1; WBSCR8; Williams-Beuren syndrome chromosomal region 8 protein
Cep-r; CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8