Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.
For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; h CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
Gene Aliases: CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8
UniProt ID: (Human) O14493
Entrez Gene ID: (Human) 1364, (Dog) 100856416, (Rat) 304407
Molecular Function:
cell junction protein
tight junction
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
