Invitrogen

Claudin 4 Monoclonal Antibody (3E2C1)

Product Image Gallery

Claudin 4 Antibody — Figure 1. Examples of CD24, CD44 and claudin 4 and 7 immunostaining in mammary invasive carcinomas (scale bar, 100 um). (A) Claudin-negative; (B) CD24-negative; (C) CD44-negative; (D) claudin 4 2+; (E) claudin 7 2+; (F) CD24-positive; (G) claudin 4 3+; (H) claudin 7 3+; (I) CD44-positive.

Claudin 4 Antibody in Western Blot (WB) — Western blot analysis of Claudin-4 was performed by loading 20 µg of Mouse Small Intestine (lane1) and PC-3 (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4°C overnight. Claudin-4 was detected at ~27 kDa using Claudin-4 Mouse Monoclonal Antibody (Product # 32-9400) at 1-3 µg/mL in 2.5 % skim milk for 3 hours at room temperature on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

2-3 µg/mL

Western Blot (WB)

1-3 µg/mL

Published Applications

Miscellaneous PubMed (MISC)

See 26 publications below

Immunofluorescence (IF)

See 33 publications below

Immunohistochemistry (IHC)

See 29 publications below

Western Blot (WB)

See 66 publications below

Immunocytochemistry (ICC)

See 27 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 35 publications below

Immunoprecipitation (IP)

See 3 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 9 publications below

Peptide Array (Array)

See 1 publication below

Product Specifications

Species Reactivity

Dog, Human, Rat

Published species

Dog, Fish, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Virus

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E2C1

Immunogen

Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533096

Product Specific Information

Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.

For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.

This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.

Target Information

CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: claudin 4; Claudin-4; CLAUDIN4; CLDN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; CPER; CPETR; CPETR1; WBSCR8; Williams-Beuren syndrome chromosomal region 8 protein

Gene Aliases: CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8

UniProt ID: (Human) O14493

Entrez Gene ID: (Human) 1364, (Dog) 100856416, (Rat) 304407

Molecular Function: cell junction protein tight junction