Immunohistochemistry analysis of Claudin 4 showing staining in the tight junctions of paraffin-embedded human colon tissue. To expose target proteins, antigen retrieval was performed by heating the tissue to 95 C in 10mM sodium citrate (pH 6.0) for 20 min. Following antigen retrieval, tissues were blocked in 3% H2O2 for 10 min at room temperature, washed with ddH2O and PBS, and then blocked with 10% goat serum for 30 min. The tissue was then probed with a Claudin 4 (green) monoclonal antibody (Product # 329488) diluted in 10% goat serum at a dilution of 3 µg/mL for 1 hour. Tissues were washed extensively in PBST and detection of the nuclei (blue) was performed using DAPI. Tissues were dehydrated with ethanol and mounted.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Dog, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.
329488 was used in immunocytochemistry and western blot to identify the members of the tight junction proteome
|Fredriksson K,Van Itallie CM,Aponte A,Gucek M,Tietgens AJ,Anderson JM||PloS one (10:null)||2015|
|Not Applicable||Not Cited||
Differential expression of claudin tight junction proteins in the human cortical nephron.
329488 was used in immunohistochemistry to study claudin tight junction proteins and differential expression in the human cortical nephron
|Kirk A,Campbell S,Bass P,Mason J,Collins J||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (25:2107)||2010|