Invitrogen

Claudin 4 Polyclonal Antibody (ZMD.306)

Product Image Gallery

Claudin 4 Antibody in Western Blot (WB) — Western blot analysis of (left) mouse and (right rat kidney homogenates using Rabbit anti-Claudin-4 Polyclonal Antibody (Product # 36-4800).

Claudin 4 Antibody — Figure 2 Establishment of claudin-1 (CLDN-1) knockout clones in Caco-2 cells. ( A ) Genomic DNA isolated from wild-type Caco-2 cells or CLDN-1 knockout clones was subjected to PCR for amplification of the CRISPR-Cas9 targeting site in the cldn-1 gene. After purifying and annealing the PCR products, the products were incubated with T7 endonuclease I, which recognizes and cleaves mismatched DNA. Two shorter bands of the predicted size were generated in some clones, indicating that CRISPR-Cas9 had successfully introduced indel mutations at the targeted cldn-1 site. This analysis indicated that the Ko-C1 clone contains only WT alleles, but the Ko-T1 and Ko-C12 clones had deletion alleles. ( B ) Immunoblots comparing CLDN-1 and CLDN-4 production by control Caco-2 cells and CLDN-1 knockout clones. These results indicated that the Ko-C12 clone completely lost the ability to produce CLDN-1, so this clone was selected for further experiments. ( C ) Immunoblot analysis comparing production of other tight junction proteins, including claudin-3 (CLDN-3), claudin-4 (CLDN-4), occludin and ZO-1, by CLDN-1 mutant cells vs. wild-type Caco-2 cells. GAPDH and E-cadherin were used as endogenous controls. For these blots, target proteins in cell lysates (30 ug protein/sample) were detected by Western blotting using specific primary antibodies and suitable horseradish peroxidase-labeled secondary antibodies. Results shown are representative of three repetitions.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 4 publications 4 publications

Immunocytochemistry (ICC)

View 6 publications 6 publications

Immunofluorescence (IF)

Assay Dependent

View 17 publications 17 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

1-3 µg/mL

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Dog, Fish, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

ZMD.306

Immunogen

Synthetic peptide derived from the C-terminal region of mouse Claudin-4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533262

Product Specific Information

Reactivity has been confirmed with mouse and rat kidney homogenates. On western blots a 22 kDa band is observed and a 44 kDa band representing dimerized protein may be observed.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl^- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.