Invitrogen

Claudin 4 Polyclonal Antibody (ZMD.306)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

40 Published Figures

95 References

View all (16) Claudin 4 antibodies

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Claudin 4 Antibody in Western Blot (WB) — Western blot analysis of (left) mouse and (right rat kidney homogenates using Rabbit anti-Claudin-4 Polyclonal Antibody (Product # 36-4800).

Claudin 4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 7 The established epithelial barrier responds in a time- and concentration-dependent manner to IFNgamma, a barrier-disruptive agent. ( A ) Representative phase-contrast images of the colonic epithelial monolayer 48 hours after treatment with IFNgamma. Gradual degeneration of the morphology of the epithelial cells was observed as the concentration of IFNgamma increased, indicating a transition from columnar to the squamous-shaped epithelium ( red arrows ). ( B ) The P app of epithelial cells to 3 kilodaltons dextran Cascade blue, over the course of 72 hours of basolateral stimulation with IFNgamma, indicates a dose-, donor-, and time-dependent barrier disruption. Data shown correspond to 1 representative of 3 independent experiments. n = 3-12 chips/condition, means +- 95% CI, 2-way analysis of variance, Tukey post hoc test. ( C ) Quantification of the cleaved over total caspase 3 in epithelial cells shows a significant and concentration-dependent activation of apoptosis 48 and 72 hours after stimulation across 3 different donors. n = 3 chips/condition means +- 95% CI, 2-way analysis of variance, Tukey post hoc test. ( D ) Representative tile fluorescent images highlighting the area of the Colon Intestine-Chip covered with epithelial cells, determined by positive 4',6-diamidino-2-phenylindole (DAPI) staining (red), 48 hours after stimulation with different concentrations of IFNgamma. The epithelial layer confluency is expressed as a percentage of the DAPI-positive area o

Claudin 4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 2 IL-1beta Signaling Directly Promotes Reprogramming of AT2 Cells (A) Schematic of organoid co-culture of SPC lineage-labeled AT2 cells (SPC + Tomato + ) with interstitial macrophages (IMs) or alveolar macrophages (AMs) isolated from wild-type lung tissue in the presence of stromal cells. See also Figure S2 . (B) Representative fluorescence images (left and center) and H&E staining (right) of AT2 organoids. GM-CSF was added to activate macrophages. Scale bars, 1,000 mum (left) and 50 mum (right). (C) Statistical quantification of colony formation efficiency and size of organoids. Each individual dot represents one experiment from one mouse, and data are presented as mean and SEM. *** p < 0.001. (D) Representative fluorescence images (top) and H&E staining (bottom) of primary organoids derived from SPC lineage-labeled AT2 cells (SPC + Tomato + ) treated with vehicle (PBS), IL-1beta, or IL-18. Scale bars, 1,000 mum (top) and 50 mum (bottom). (E) Quantification of colony formation efficiency and size. Data are presented as mean and SEM. (F) UMAP visualization of cell clusters from scRNA-seq analysis of epithelial cells from control (1,286 cells) or IL-1beta-treated organoids (10 ng/mL, 2,584 cells). Cells were isolated on day 21 in organoid culture. Colors indicate samples and distinct cell types. The number of cells in the individual cluster is depicted. See also Figure S3 . (G) The percentage of each cluster in total cells of control or IL-1beta-treated organoids. (H) D

Claudin 4 Antibody in Immunocytochemistry (ICC/IF) — Figure 5 ZIKV added to the basolateral surface of BeWo cells transiently diminishes the transepithelial electrical resistance and claudin-4 expression. Confluent monolayers of BeWo cells that had achieved a stable value of TER were incubated with ZIKV (MOI = 1) added to the apical or basolateral surface. ( a ) TER was continuously measured in the cellZscope system in three inserts per condition. Results are shown with the corresponding standard deviation. ( b ) Immunofluorescence for claudin-4, occludin and ZO-1 in BeWo monolayers done after 1.5 h of incubation with ZIKV. Bar, 50 mum. X-Y, en face view; X-Z, lateral view.

Claudin 4 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Figure 3 Representative photographs of claudin-2, claudin-3, claudin-4, and zonula occludens-1 immunostained in longitudinal jejunal sections of the three experimental groups. Claudin-2 immunoreaction in the sham-operated group (a) was low and was mainly found in the cytoplasm of the intestinal epithelial cells. Claudin-2 expression in the groups exposed to ischemia-reperfusion was substantially higher and was principally found in the lateral membrane of the epithelial cells (b, c). The sham-operated group presented strong membrane claudin-3 immunostaining (d), which was remarkably decreased in the ischemia-reperfusion- (IR-) group (e), but not in the postconditioned- (PC-) group (f). Minimal claudin-4 immunostaining was observed in the jejunal samples of the sham-operated animals (g). The two groups challenged with 60 minutes of mesenteric ischemia and 6 hours of reperfusion (h, i) showed high membrane claudin-4 expression, which was limited to the tips of the villi. Zonula occludens-1 was strongly immunostained in the jejunal samples of the sham-operated animals (j). Following small intestinal ischemia-reperfusion, intestinal epithelial cell with zonula occludens-1 membrane immunostaining notably decreased in the IR-group (k); in the PC-group minimal loss of zonula occludens-1 membrane immunostaining was detected (l). Each photograph was taken with the same magnification. Scale bar: 20 mu m.

Claudin 4 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — EV4 Figure Aberrant activation of YAP/TAZ-mTORC1 signaling in the human lungs of idiopathic pulmonary fibrosis (IPF) Representative IF images showing the expressions of AT1 cell marker AQP5 and CAV1 in the airways of normal background (top), IPF (middle), and BO (bottom) human lungs. AQP5 (red), CAV1 (white), and DAPI (blue). Scale bars, 100 mum. UMAP plot showing single cells from normal background (control, 5552) and pulmonary fibrosis (PF, 12477) of human lung tissues (GSE135893). UMAP plot representing 5 color-coded cell clusters identified in merged single-cell transcriptomes from control and PF lungs. Blob plot depicting selected marker genes in each cell cluster. Dot size encodes the percentage of cells expressing the genes, and color encodes the average per cell gene expression level. Violin plots showing normalized expression levels of YAP/TAZ targets ( CYR61, AMOTL2, CTGF, GAS6 ) and mTORC1-ATF4 targets ( ATF4, ATF3, PPPR15A, SLC3A2 ). Representative H&E images of human airway from normal background or IPF. Scale bars, 100 mum. Representative IF images showing the expression of YAP, ATF4, DATP marker CLDN4, AT1 cell marker AQP5, secretory cell marker CC10, and mTORC1 activity marker p-S6 in human lungs of IPF. YAP (green, top and bottom), ATF4 (white, bottom), CLDN4 (white, top), AQP5 (red, top and bottom), CC10 (green, middle), p-S6 (white, middle), and DAPI (blue). Scale bars, 100 mum.

Claudin 4 Antibody in Immunohistochemistry (IHC) — Fig. 3 PM2.5 is likely to influence the repair of alveolar damage by affecting the transdifferentiation of AT2 to AT1. A The culture process of mouse alveolar organoids. Scale bar = 500 mum or 100 mum as indicated. B Immunofluorescence staining of alveolar organoids in CONTROL group and PM2.5-exposed alveolar organoids for HOPX, SPC and Ki67 in the alveolar differentiation phase day 4. Scale bar = 50 mum. C Quantitative of AT1/AT2 ratio per 20 x field. D Quantitative of Ki67 + /total ratio per 20 x field. E , F The mRNA expression of cell cycle related genes Cdc25c and Cyclin B1 in the alveolar differentiation phase day 4. G Immunofluorescence staining of intermediate AT2-AT1 cell process marker CLDN4 in the alveolar differentiation phase day 4. Scale bar = 50 mum. **p < 0.01, ***p < 0.001, ****p < 0.0001. Comparisons between two groups (CONTROL vs PM2.5 exposure) were analyzed by unpaired t test. Data presented are means +- SD. The experiments were repeated at least three times (n >= 3)

Claudin 4 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 1 Claudin-4 present at the basal surface of the STB layer diminished in ZIKV-infected placentae. ( a ) Frozen sections of human placentae derived from women infected or not (control) with ZIKV were processed for immunofluorescence with a rabbit antibody against claudin-4 (Cl-4) and a mouse antibody anti cytokeratin 18 (K18). DNA of nuclei was stained with DAPI. Apical surface of STB cell layer (arrow); basolateral surface of STB cell layer (arrowhead); intervillous space (asterisk). Bar, 50 mum; magnification bar, 25 mum. ( b ) Measurements of mean fluorescence intensity of the trophoblast layer were done on three independent images from each condition. Since the variances were equal among the two groups, we applied a Student's t -test. To include effect size estimation, since both groups have similar standard deviations, we used Cohen's d . The three values obtained per donor are represented by dots with the same color. ** p = 0.0054, Cohen's d = 3 indicating that 99.9% of the values from the ZIKV group are below the mean value of the control group. ( c ) Representative Western blot of three independent experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was employed as loading control. ( d ) Densitometric analysis of Western blots. The three values obtained per donor are represented by dots with the same color. ** p = 0.00092 Cohen's d = 2.67 indicating that 99.9% of the values from the ZIKV group are below the mean value of the control group.

Claudin 4 Antibody — 25D3 mediated signaling in EGFR mutant, HCC827 cells is dependent upon the VDR HCC827 cells were transfected with either non-targeting control siRNA (siCTRL) or siRNA targeting the VDR (siVDR). Twenty four h later, cells were treated with EtOH vehicle control, 100 nM 1,25D3 or 1 µM 25D3. RNA or protein was extracted 6 h post treatment. qRT-PCR and Western blot assays were performed to study vitamin D regulated genes and to confirm VDR knockdown. The VDR knockdown experiment was repeated 4 times. The results that are presented are from a representative experiment. {KD}

Product Details

36-4800

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 31 publications 31 publications
Immunocytochemistry (ICC/IF)	Assay-dependent	View 19 publications 19 publications
ELISA (ELISA)	0.1-1 µg/mL	-
Immunoprecipitation (IP)	1-3 µg/mL	-
Immunohistochemistry (IHC)	-	View 29 publications 29 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 5 publications 5 publications
Flow Cytometry (Flow)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 4 publications 4 publications
Miscellaneous PubMed (Misc)	-	View 5 publications 5 publications

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Dog, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rat, Sheep
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Clone	ZMD.306
Immunogen	Synthetic peptide derived from the C-terminal region of mouse Claudin-4 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533262

Product Specific Information

Reactivity has been confirmed with mouse and rat kidney homogenates. On western blots a 22 kDa band is observed and a 44 kDa band representing dimerized protein may be observed.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl^- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Airway secretory cell fate conversion via YAP-mTORC1-dependent essential amino acid metabolism.

The EMBO journal

Jeon HY,Choi J,Kraaier L,Kim YH,Eisenbarth D,Yi K,Kang JG,Kim JW,Shim HS,Lee JH,Lim DS

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Apr 19 00:00:00 EDT 2022

Airway secretory cell fate conversion via YAP-mTORC1-dependent essential amino acid metabolism.

The EMBO journal

Jeon HY,Choi J,Kraaier L,Kim YH,Eisenbarth D,Yi K,Kang JG,Kim JW,Shim HS,Lee JH,Lim DS

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Apr 19 00:00:00 EDT 2022

A Novel Microphysiological Colon Platform to Decipher Mechanisms Driving Human Intestinal Permeability.

Cellular and molecular gastroenterology and hepatology

Apostolou A,Panchakshari RA,Banerjee A,Manatakis DV,Paraskevopoulou MD,Luc R,Abu-Ali G,Dimitriou A,Lucchesi C,Kulkarni G,Maulana TI,Kasendra M,Kerns JS,Bleck B,Ewart L,Manolakos ES,Hamilton GA,Giallourakis C,Karalis K

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Mon Mar 28 00:00:00 EDT 2022

A Novel Microphysiological Colon Platform to Decipher Mechanisms Driving Human Intestinal Permeability.

Cellular and molecular gastroenterology and hepatology

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Mon Mar 28 00:00:00 EDT 2022

Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease.

Respiratory research

Yu H,Lin Y,Zhong Y,Guo X,Lin Y,Yang S,Liu J,Xie X,Sun Y,Wang D,Li B,Ran P,Dai J

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Fri Mar 25 00:00:00 EDT 2022

B. adolescentis ameliorates chronic colitis by regulating Treg/Th2 response and gut microbiota remodeling.

Gut microbes

Fan L,Qi Y,Qu S,Chen X,Li A,Hendi M,Xu C,Wang L,Hou T,Si J,Chen S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jan 18 00:00:00 EST 2022

Desmosomes polarize and integrate chemical and mechanical signaling to govern epidermal tissue form and function.

Current biology : CB

Broussard JA,Koetsier JL,Hegazy M,Green KJ

36-4800 was used in Western Blotting to demonstrate that the desmosome-IF network is a critical contributor to the cytoskeletal-adhesive machinery that supports the polarized function of the epidermis.

Mon Aug 09 00:00:00 EDT 2021

Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern.

Communications biology

Tabariès S,Annis MG,Lazaris A,Petrillo SK,Huxham J,Abdellatif A,Palmieri V,Chabot J,Johnson RM,Van Laere S,Verhoef C,Hachem Y,Yumeen S,Meti N,Omeroglu A,Altinel G,Gao ZH,Yu ASL,Grünhagen DJ,Vermeulen P,Metrakos P,Siegel PM

36-4800 was used in Immunohistochemistry to provide evidence that Claudin-2 status in patient-derived extracellular vesicles may serve as a relevant prognostic biomarker to predict whether colorectal cancer patients have developed replacement type liver metastases.

Wed Jun 02 00:00:00 EDT 2021

Tight Junction Protein Claudin-7 Is Essential for Intestinal Epithelial Stem Cell Self-Renewal and Differentiation.

Cellular and molecular gastroenterology and hepatology

Xing T,Benderman LJ,Sabu S,Parker J,Yang J,Lu Q,Ding L,Chen YH

36-4800 was used in Western Blotting to show that Cldn7 is indispensable in controlling Wnt/β-catenin signaling-dependent intestinal epithelial stem cell survival, self-renewal, and cell differentiation.

Mon May 10 00:00:00 EDT 2021

Quercetin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes.

Nutrients

Jia H,Zhang Y,Si X,Jin Y,Jiang D,Dai Z,Wu Z

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jan 26 00:00:00 EST 2021

Quercetin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes.

Nutrients

Jia H,Zhang Y,Si X,Jin Y,Jiang D,Dai Z,Wu Z

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jan 26 00:00:00 EST 2021

Citrus limon Peel Powder Reduces Intestinal Barrier Defects and Inflammation in a Colitic Murine Experimental Model.

Foods (Basel, Switzerland)

Tinh NTT,Sitolo GC,Yamamoto Y,Suzuki T

36-4800 was used in Western Blot to examine the ameliorative effects of lemon (Citrus limon) peel (LP) powder on intestinal inflammation and barrier defects in dextran sulfate sodium (DSS)-induced colitic mice.

Mon Jan 25 00:00:00 EST 2021

Doxorubicin increases permeability of murine small intestinal epithelium and cultured T84 monolayers.

Scientific reports

Cray P,Sheahan BJ,Cortes JE,Dekaney CM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Dec 08 00:00:00 EST 2020

Doxorubicin increases permeability of murine small intestinal epithelium and cultured T84 monolayers.

Scientific reports

Cray P,Sheahan BJ,Cortes JE,Dekaney CM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Dec 08 00:00:00 EST 2020

E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions.

International journal of oncology

Uc PY,Miranda J,Raya-Sandino A,Alarcón L,Roldán ML,Ocadiz-Delgado R,Cortés-Malagón EM,Chávez-Munguía B,Ramírez G,Asomoza R,Shoshani L,Gariglio P,González-Mariscal L

36-4800 was used in Western Blotting to investigate the role of human papillomavirus (HPV) 16 E7 oncoprotein on the sealing of TJs, and also the expression level of claudins in mouse cervix and in epithelial Madin‚Darby Canine Kidney (MDCK) cells.

Thu Oct 01 00:00:00 EDT 2020

Impact of Sensitization and Inflammation on the Interaction of Mast Cells With the Intestinal Epithelium in Rats.

Frontiers in physiology

Becker J,Ott D,Diener M

36-4800 was used in Immunohistochemistry-immunofluorescence to study the impact of sensitisation and inflammation on the interaction of mast cells with the intestinal epithelium in rats.

Wed Sep 30 00:00:00 EDT 2020

Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration.

Cell stem cell

Choi J,Park JE,Tsagkogeorga G,Yanagita M,Koo BK,Han N,Lee JH

36-4800 was used in Immunohistochemistry (Paraffin) to map the trajectories of alveolar-lineage cells during injury repair and lung regeneration by single-cell RNA sequencing in combination with in vivo lineage tracing and organoid models.

Thu Sep 03 00:00:00 EDT 2020

Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration.

Cell stem cell

Choi J,Park JE,Tsagkogeorga G,Yanagita M,Koo BK,Han N,Lee JH

Thu Sep 03 00:00:00 EDT 2020

Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration.

Cell stem cell

Choi J,Park JE,Tsagkogeorga G,Yanagita M,Koo BK,Han N,Lee JH

Thu Sep 03 00:00:00 EDT 2020

Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration.

Cell stem cell

Choi J,Park JE,Tsagkogeorga G,Yanagita M,Koo BK,Han N,Lee JH

Thu Sep 03 00:00:00 EDT 2020

Emergence of a High-Plasticity Cell State during Lung Cancer Evolution.

Cancer cell

Marjanovic ND,Hofree M,Chan JE,Canner D,Wu K,Trakala M,Hartmann GG,Smith OC,Kim JY,Evans KV,Hudson A,Ashenberg O,Porter CBM,Bejnood A,Subramanian A,Pitter K,Yan Y,Delorey T,Phillips DR,Shah N,Chaudhary O,Tsankov A,Hollmann T,Rekhtman N,Massion PP,Poirier JT,Mazutis L,Li R,Lee JH,Amon A,Rudin CM,Jacks T,Regev A,Tammela T

36-4800 was used in Immunohistochemistry to profile cellular transcriptomes of lung tumours at different stages.

Mon Aug 10 00:00:00 EDT 2020

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Nature cell biology

Kobayashi Y,Tata A,Konkimalla A,Katsura H,Lee RF,Ou J,Banovich NE,Kropski JA,Tata PR

36-4800 was used in Immunocytochemistry-Immunofluorescence to implicate a transient state associated with senescence in normal epithelial tissue repair and its abnormal persistence in disease conditions.

Sat Aug 01 00:00:00 EDT 2020

NOTCH1 signaling establishes the medullary thymic epithelial cell progenitor pool during mouse fetal development.

Development (Cambridge, England)

Li J,Gordon J,Chen ELY,Xiao S,Wu L,Zúñiga-Pflücker JC,Manley NR

36-4800 was used in Immunocytochemistry to provide strong evidence for a requirement for notch signaling in specification of the mTEC lineage.

Mon Jun 22 00:00:00 EDT 2020

Hyaluronan 35 kDa enhances epithelial barrier function and protects against the development of murine necrotizing enterocolitis.

Pediatric research

Gunasekaran A,Eckert J,Burge K,Zheng W,Yu Z,Kessler S,de la Motte C,Chaaban H

36-4800 was used in Immunohistochemistry to investigate the effects of HA 35 kDa on a NEC-like murine model.

Mon Jun 01 00:00:00 EDT 2020

Resolvin E1 is a pro-repair molecule that promotes intestinal epithelial wound healing.

Proceedings of the National Academy of Sciences of the United States of America

Quiros M,Feier D,Birkl D,Agarwal R,Zhou DW,García AJ,Parkos CA,Nusrat A

36-4800 was used in Western Blotting to investigate the role for RvE1 in regulating intestinal epithelial cell migration and proliferation during mucosal wound repair.

Tue Apr 28 00:00:00 EDT 2020

Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection.

Cellular and molecular gastroenterology and hepatology

Suzuki K,Sentani K,Tanaka H,Yano T,Suzuki K,Oshima M,Yasui W,Tamura A,Tsukita S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 08 00:00:00 EDT 2020

Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection.

Cellular and molecular gastroenterology and hepatology

Suzuki K,Sentani K,Tanaka H,Yano T,Suzuki K,Oshima M,Yasui W,Tamura A,Tsukita S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 08 00:00:00 EDT 2020

Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro.

Toxicology in vitro : an international journal published in association with BIBRA

Kobayashi K,Tsugami Y,Suzuki N,Suzuki T,Nishimura T

36-4800 was used in Immunohistochemistry to investigate whether nicotine directly affects milk production in lactating mammary epithelial cells.

Sun Mar 01 00:00:00 EST 2020

Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.

Gut microbes

Jang YJ,Kim WK,Han DH,Lee K,Ko G

36-4800 was used in Western Blotting to evaluate immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.

Mon Feb 10 00:00:00 EST 2020

The Differentiation-Associated Keratinocyte Protein Cornifelin Contributes to Cell-Cell Adhesion of Epidermal and Mucosal Keratinocytes.

The Journal of investigative dermatology

Wagner T,Beer L,Gschwandtner M,Eckhart L,Kalinina P,Laggner M,Ellinger A,Gruber R,Kuchler U,Golabi B,Tschachler E,Mildner M

36-4800 was used in Western Blotting to indicate that CNFN is a structural component of the cell adhesion system of differentiated KCs in both epidermis and oral mucosa.

Fri Nov 01 00:00:00 EDT 2019

Effects of Claudin-1 on the Action of Clostridium perfringens Enterotoxin in Caco-2 Cells.

Toxins

Mehdizadeh Gohari I,Li J,Navarro M,Uzal F,McClane B

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 09 00:00:00 EDT 2019

Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells.

International journal of molecular sciences

Gamero-Estevez E,Andonian S,Jean-Claude B,Gupta I,Ryan AK

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 02 00:00:00 EDT 2019

Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells.

International journal of molecular sciences

Gamero-Estevez E,Andonian S,Jean-Claude B,Gupta I,Ryan AK

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 02 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Miranda J,Martín-Tapia D,Valdespino-Vázquez Y,Alarcón L,Espejel-Nuñez A,Guzmán-Huerta M,Muñoz-Medina JE,Shibayama M,Chávez-Munguía B,Estrada-Gutiérrez G,Lievano S,Ludert JE,González-Mariscal L

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sun Sep 29 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sun Sep 29 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sun Sep 29 00:00:00 EDT 2019

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

The kidney anion exchanger 1 affects tight junction properties via claudin-4.

Scientific reports

Lashhab R,Rumley AC,Arutyunov D,Rizvi M,You C,Dimke H,Touret N,Zimmermann R,Jung M,Chen XZ,Alexander T,Cordat E

36-4800 was used in Western Blotting to elucidate a novel interaction between claudin-4 and the kidney anion exchanger, which may be relevant to ion and/or pH homeostasis.

Thu Feb 28 00:00:00 EST 2019

Immunoexpression of claudins 4 and 7 among invasive breast carcinoma subtypes: A large diagnostic study using tissue microarray.

Molecular and clinical oncology

Logullo AF,Pasini FS,Nonogaki S,Rocha RM,Soares FA,Brentani MM

36-4800 was used in Immunohistochemistry-immunofluorescence to study whether the immunoexpression of claudins 4 and 7 in tissue microarray is associated with survival and prognosis in luminal A, HER-2 and triple-negative molecular breast cancer subtypes.

Mon Oct 01 00:00:00 EDT 2018

The Human Blood-Nerve Barrier Transcriptome.

Scientific reports

Palladino SP,Helton ES,Jain P,Dong C,Crowley MR,Crossman DK,Ubogu EE

36-4800 was used in Immunocytochemistry-immunoflourescence to sequence the human blood-nerve barrier transcriptome using confluent P3 and P8 pHEndEC monolayers and normal adult laser-capture microdissected endoneurial microvessels.

Tue Dec 12 00:00:00 EST 2017

Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.

Oncology letters

Shimada H,Satohisa S,Kohno T,Konno T,Takano KI,Takahashi S,Hatakeyama T,Arimoto C,Saito T,Kojima T

36-4800 was used in Western Blotting to indicate that the downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion of human endometrial cancer via claudin-1 mediation of matrix metalloproteinases.

Fri Dec 01 00:00:00 EST 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Jul 11 00:00:00 EDT 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Jul 11 00:00:00 EDT 2017

Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium.

The EMBO journal

Miyoshi H,VanDussen KL,Malvin NP,Ryu SH,Wang Y,Sonnek NM,Lai CW,Stappenbeck TS

364800 was used in immunocytochemistry to study wound-associated epithelial cell differentiation

Wed Jan 04 00:00:00 EST 2017

Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium.

The EMBO journal

Miyoshi H,VanDussen KL,Malvin NP,Ryu SH,Wang Y,Sonnek NM,Lai CW,Stappenbeck TS

364800 was used in immunocytochemistry to study wound-associated epithelial cell differentiation

Wed Jan 04 00:00:00 EST 2017

Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.

Journal of veterinary science

Lee B,Kang HY,Lee DO,Ahn C,Jeung EB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Fri Dec 30 00:00:00 EST 2016

Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.

Journal of veterinary science

Lee B,Kang HY,Lee DO,Ahn C,Jeung EB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Fri Dec 30 00:00:00 EST 2016

Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with β-casein expression in mammary epithelial cells.

Biochimica et biophysica acta

Kobayashi K,Tsugami Y,Matsunaga K,Oyama S,Kuki C,Kumura H

36-4800 was used in immunohistochemistry - paraffin section to characterize induction of lactation-specific tight junctions concurrent with beta-casein expression in mammary epithelial cells due to prolactin and glucocorticoid signaling

Mon Aug 01 00:00:00 EDT 2016

Connexins, E-cadherin, Claudin-7 and β-catenin transiently form junctional nexuses during the post-natal mammary gland development.

Developmental biology

Dianati E,Poiraud J,Weber-Ouellette A,Plante I

36-4800 was used in immunohistochemistry - frozen section and western blot to assess the transiently formed junctional nexuses during post-natal mammary gland development by E-cadherins, connexins, beta-catenin, and claudin-7

Mon Aug 01 00:00:00 EDT 2016

Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.

PloS one

Fredriksson K,Van Itallie CM,Aponte A,Gucek M,Tietgens AJ,Anderson JM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Fri Apr 29 00:00:00 EDT 2016

Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.

PloS one

Fredriksson K,Van Itallie CM,Aponte A,Gucek M,Tietgens AJ,Anderson JM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Fri Apr 29 00:00:00 EDT 2016

Diurnal variation of tight junction integrity associates inversely with matrix metalloproteinase expression in Xenopus laevis corneal epithelium: implications for circadian regulation of homeostatic surface cell desquamation.

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

The Journal of cell biology

Gao X,Bali AS,Randell SH,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Nov 09 00:00:00 EST 2015

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

The Journal of cell biology

Gao X,Bali AS,Randell SH,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Nov 09 00:00:00 EST 2015

Diverse regulation of claudin-1 and claudin-4 in atopic dermatitis.

The American journal of pathology

Gruber R,Börnchen C,Rose K,Daubmann A,Volksdorf T,Wladykowski E,Vidal-Y-Sy S,Peters EM,Danso M,Bouwstra JA,Hennies HC,Moll I,Schmuth M,Brandner JM

36-4800 was used in immunohistochemistry (paraffin) to study how Cldn-1 and Cldn-4 are altered in atopic dermatitis.

Thu Oct 01 00:00:00 EDT 2015

A non-tight junction function of claudin-7-Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment.

Molecular cancer

Lu Z,Kim DH,Fan J,Lu Q,Verbanac K,Ding L,Renegar R,Chen YH

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Jun 17 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Jun 15 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Jun 15 00:00:00 EDT 2015

Mode of action of claudin peptidomimetics in the transient opening of cellular tight junction barriers.

Biomaterials

Staat C,Coisne C,Dabrowski S,Stamatovic SM,Andjelkovic AV,Wolburg H,Engelhardt B,Blasig IE

36-4800 was used in immunocytochemistry to investigate the role of claudin-1/claudin-5 in epithelial/endothelial barriers

Mon Jun 01 00:00:00 EDT 2015

NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation.

Tissue barriers

Ward C,Schlingmann B,Stecenko AA,Guidot DM,Koval M

36-4800 was used in Western Blotting to suggest NF-κB regulates alveolar barrier function and that constitutive NF-κB function is required for alveolar epithelial tight junction integrity.

Fri Apr 03 00:00:00 EDT 2015

Impaired intestinal mucosal barrier upon ischemia-reperfusion: "patching holes in the shield with a simple surgical method".

BioMed research international

Rosero O,Ónody P,Kovács T,Molnár D,Lotz G,Tóth S,Turóczi Z,Fülöp A,Garbaisz D,Harsányi L,Szijártó A

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Thu Feb 12 00:00:00 EST 2015

Impaired intestinal mucosal barrier upon ischemia-reperfusion: "patching holes in the shield with a simple surgical method".

BioMed research international

Rosero O,Ónody P,Kovács T,Molnár D,Lotz G,Tóth S,Turóczi Z,Fülöp A,Garbaisz D,Harsányi L,Szijártó A

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Thu Feb 12 00:00:00 EST 2015

EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells.

Journal of cellular physiology

García-Hernández V,Flores-Maldonado C,Rincon-Heredia R,Verdejo-Torres O,Bonilla-Delgado J,Meneses-Morales I,Gariglio P,Contreras RG

36-4800 was used in immunocytochemistry and western blot to investigate the effect of EGF on claudin-2 and -4 expression in MDCK cells.

Thu Jan 01 00:00:00 EST 2015

Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice.

Cell death & disease

Ruan L,Zhang Z,Mu L,Burnley P,Wang L,Coder B,Zhuge Q,Su DM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 09 00:00:00 EDT 2014

Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice.

Cell death & disease

Ruan L,Zhang Z,Mu L,Burnley P,Wang L,Coder B,Zhuge Q,Su DM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 09 00:00:00 EDT 2014

Enteral supplementation of bovine lactoferrin improves gut barrier function in rats after massive bowel resection.

The British journal of nutrition

Wu J,Chen J,Wu W,Shi J,Zhong Y,van Tol EA,Tang Q,Cai W

36-4800 was used in western blot to assess the effect of enteral bovine lactoferrin supplementation on intestinal adaptation and barrier function in a rat model of short bowel syndrome.

Thu Aug 28 00:00:00 EDT 2014

Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.

Development (Cambridge, England)

Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER

36-4800 was used in immunohistochemistry - paraffin section to assess the contribution of Tbx1 to thymus and parathyroid development

Fri Aug 01 00:00:00 EDT 2014

Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.

Development (Cambridge, England)

Castillo D,Seidel K,Salcedo E,Ahn C,de Sauvage FJ,Klein OD,Barlow LA

36-4800 was used in immunohistochemistry - frozen section to examine the effects of sonic hedgehog on the lingual epithelium of adult mice

Fri Aug 01 00:00:00 EDT 2014

Role of the p63-FoxN1 regulatory axis in thymic epithelial cell homeostasis during aging.

Cell death & disease

Burnley P,Rahman M,Wang H,Zhang Z,Sun X,Zhuge Q,Su DM

36-4800 was used in immunohistochemistry (frozen) to assess the contributions of the p63 gene and FoxN1 to thymic epithelial cell homeostasis during thymic aging.

Thu Nov 21 00:00:00 EST 2013

Gastroesophageal reflux activates the NF-κB pathway and impairs esophageal barrier function in mice.

American journal of physiology. Gastrointestinal and liver physiology

Fang Y,Chen H,Hu Y,Djukic Z,Tevebaugh W,Shaheen NJ,Orlando RC,Hu J,Chen X

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Mon Jul 01 00:00:00 EDT 2013

Gastroesophageal reflux activates the NF-κB pathway and impairs esophageal barrier function in mice.

American journal of physiology. Gastrointestinal and liver physiology

Fang Y,Chen H,Hu Y,Djukic Z,Tevebaugh W,Shaheen NJ,Orlando RC,Hu J,Chen X

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Mon Jul 01 00:00:00 EDT 2013

Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].

Proceedings of the National Academy of Sciences of the United States of America

Gao X,Vockley CM,Pauli F,Newberry KM,Xue Y,Randell SH,Reddy TE,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Jun 04 00:00:00 EDT 2013

Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].

Proceedings of the National Academy of Sciences of the United States of America

Gao X,Vockley CM,Pauli F,Newberry KM,Xue Y,Randell SH,Reddy TE,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Jun 04 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed May 01 00:00:00 EDT 2013

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Aging and disease

Xia J,Wang H,Guo J,Zhang Z,Coder B,Su DM

36-4800 was used in immunohistochemistry to test if disruption of the steady-state thymic medulla results in the increased autoimmunity associated with age.

Fri Jun 01 00:00:00 EDT 2012

CD44 regulates tight-junction assembly and barrier function.

The Journal of investigative dermatology

Kirschner N,Haftek M,Niessen CM,Behne MJ,Furuse M,Moll I,Brandner JM

Fri Apr 01 00:00:00 EDT 2011

Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-ζ and PP2A pathway.

American journal of physiology. Lung cellular and molecular physiology

Caraballo JC,Yshii C,Butti ML,Westphal W,Borcherding JA,Allamargot C,Comellas AP

Fri Apr 01 00:00:00 EDT 2011

Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium.

Journal of molecular medicine (Berlin, Germany)

Guo J,Rahman M,Cheng L,Zhang S,Tvinnereim A,Su DM

36-4800 was used in flow cytometry and immunohistochemistry - paraffin section to elucidate how FoxN1 contributes to cyst formation and thyroid development

Tue Mar 01 00:00:00 EST 2011

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy.

Reproduction (Cambridge, England)

Bailey DW,Dunlap KA,Frank JW,Erikson DW,White BG,Bazer FW,Burghardt RC,Johnson GA

36-4800 was used in Immunofluorescence in immunohistochemistry on frozen tissues to show that progesterone affects luminal epithelium, glandular epithelium (GE), and vascular development and histoarchitecture.

Fri Oct 01 00:00:00 EDT 2010

Cell polarity regulator PARD6B is essential for trophectoderm formation in the preimplantation mouse embryo.

Biology of reproduction

Alarcon VB

36-4800 was used in immunocytochemistry to elucidate the role of partitioning defective genes in trophectoderm differentiation and blastocyst formation

Wed Sep 01 00:00:00 EDT 2010

Postnatal tissue-specific disruption of transcription factor FoxN1 triggers acute thymic atrophy.

The Journal of biological chemistry

Cheng L,Guo J,Sun L,Fu J,Barnes PF,Metzger D,Chambon P,Oshima RG,Amagai T,Su DM

36-4800 was used in immunohistochemistry to investigate the postnatal contribution of FoxN1 to thymic maintenance

Fri Feb 19 00:00:00 EST 2010

Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.

Gastroenterology

Chang TL,Ito K,Ko TK,Liu Q,Salto-Tellez M,Yeoh KG,Fukamachi H,Ito Y

Fri Jan 01 00:00:00 EST 2010

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 29 00:00:00 EDT 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 29 00:00:00 EDT 2009

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

Altered gene expression in breast cancer liver metastases.

International journal of cancer

Erin N,Wang N,Xin P,Bui V,Weisz J,Barkan GA,Zhao W,Shearer D,Clawson GA

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2009

Altered gene expression in breast cancer liver metastases.

International journal of cancer

Erin N,Wang N,Xin P,Bui V,Weisz J,Barkan GA,Zhao W,Shearer D,Clawson GA

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2009

Phosphorylation of claudin-4 is required for tight junction formation in a human keratinocyte cell line.

Experimental cell research

Aono S,Hirai Y

36-4800 was used in immunocytochemistry and western blot to suggest that aPKC regulates tight junction formation through the phosphorylation of claudin-4

Sat Nov 01 00:00:00 EDT 2008

Tight and adherens junctions in the ovine uterus: differential regulation by pregnancy and progesterone.

Endocrinology

Satterfield MC,Dunlap KA,Hayashi K,Burghardt RC,Spencer TE,Bazer FW

36-4800 was used in Immunohistochemistry to identify changes in junctional complexes in the ovine endometrium that regulate paracellular trafficking of water, ions, and other molecules, and the secretory capacity of the uterine epithelia.

Wed Aug 01 00:00:00 EDT 2007

Claudin-based permeability barriers in taste buds.

The Journal of comparative neurology

Michlig S,Damak S,Le Coutre J

36-4800 was used in immunohistochemistry to study the role of claudin in permeability barriers in taste buds

Wed Jun 20 00:00:00 EDT 2007

Claudin-based permeability barriers in taste buds.

The Journal of comparative neurology

Michlig S,Damak S,Le Coutre J

36-4800 was used in immunohistochemistry to study the role of claudin in permeability barriers in taste buds

Wed Jun 20 00:00:00 EDT 2007

Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin.

Nature immunology

Hamazaki Y,Fujita H,Kobayashi T,Choi Y,Scott HS,Matsumoto M,Minato N

36-4800 was used in immunohistochemistry - frozen section to examine the origin and development of Aire positive medullary thymic epithelial cells

Thu Mar 01 00:00:00 EST 2007

Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines.

American journal of physiology. Cell physiology

Guo X,Rao JN,Liu L,Zou TT,Turner DJ,Bass BL,Wang JY

Sat Nov 01 00:00:00 EST 2003

Regulated expression of claudin-4 decreases paracellular conductance through a selective decrease in sodium permeability.

The Journal of clinical investigation

Van Itallie C,Rahner C,Anderson JM

Tue May 01 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; h CPE-receptor

Gene Aliases: Cep-r; Cldn4; Cper; Cpetr; Cpetr1

UniProt ID: (Mouse) O35054

Entrez Gene ID: (Rat) 304407, (Mouse) 12740

Molecular Function: tight junction

Function(s)

female pregnancy circadian rhythm response to progesterone establishment of skin barrier

Process(es)

molecular_function structural molecule activity protein binding

Claudin 4 Polyclonal Antibody (ZMD.306)

Product Details

36-4800

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunohistochemistry (Paraffin) (IHC (P))

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

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Type

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