Invitrogen

Claudin 4 Polyclonal Antibody (ZMD.306)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

40 Published Figures

84 References

View all (16) Claudin 4 antibodies

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Claudin 4 Antibody in Western Blot (WB) — Western blot analysis of (left) mouse and (right rat kidney homogenates using Rabbit anti-Claudin-4 Polyclonal Antibody (Product # 36-4800).

Claudin 4 Antibody — 25D3 mediated signaling in EGFR mutant, HCC827 cells is dependent upon the VDR HCC827 cells were transfected with either non-targeting control siRNA (siCTRL) or siRNA targeting the VDR (siVDR). Twenty four h later, cells were treated with EtOH vehicle control, 100 nM 1,25D3 or 1 µM 25D3. RNA or protein was extracted 6 h post treatment. qRT-PCR and Western blot assays were performed to study vitamin D regulated genes and to confirm VDR knockdown. The VDR knockdown experiment was repeated 4 times. The results that are presented are from a representative experiment. {KD}

Claudin 4 Antibody in Immunocytochemistry (ICC/IF) — Figure 5 ZIKV added to the basolateral surface of BeWo cells transiently diminishes the transepithelial electrical resistance and claudin-4 expression. Confluent monolayers of BeWo cells that had achieved a stable value of TER were incubated with ZIKV (MOI = 1) added to the apical or basolateral surface. ( a ) TER was continuously measured in the cellZscope system in three inserts per condition. Results are shown with the corresponding standard deviation. ( b ) Immunofluorescence for claudin-4, occludin and ZO-1 in BeWo monolayers done after 1.5 h of incubation with ZIKV. Bar, 50 mum. X-Y, en face view; X-Z, lateral view.

Claudin 4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry and Immunohistochemistry

Claudin 4 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Figure 3 Representative photographs of claudin-2, claudin-3, claudin-4, and zonula occludens-1 immunostained in longitudinal jejunal sections of the three experimental groups. Claudin-2 immunoreaction in the sham-operated group (a) was low and was mainly found in the cytoplasm of the intestinal epithelial cells. Claudin-2 expression in the groups exposed to ischemia-reperfusion was substantially higher and was principally found in the lateral membrane of the epithelial cells (b, c). The sham-operated group presented strong membrane claudin-3 immunostaining (d), which was remarkably decreased in the ischemia-reperfusion- (IR-) group (e), but not in the postconditioned- (PC-) group (f). Minimal claudin-4 immunostaining was observed in the jejunal samples of the sham-operated animals (g). The two groups challenged with 60 minutes of mesenteric ischemia and 6 hours of reperfusion (h, i) showed high membrane claudin-4 expression, which was limited to the tips of the villi. Zonula occludens-1 was strongly immunostained in the jejunal samples of the sham-operated animals (j). Following small intestinal ischemia-reperfusion, intestinal epithelial cell with zonula occludens-1 membrane immunostaining notably decreased in the IR-group (k); in the PC-group minimal loss of zonula occludens-1 membrane immunostaining was detected (l). Each photograph was taken with the same magnification. Scale bar: 20 mu m.

Claudin 4 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 3 Tight junction proteins occludin and claudin-4 are co-expressed in Xenopus corneal epithelium lateral membranes and are disrupted at night. Double-label confocal immunocytochemistry was performed on whole flat-mounted preparations of Xenopus corneas that were obtained from animals in the late afternoon (DAY; 9 hours after lights on in a 12L:12D cycle) and in the late night (NIGHT; 3 hours before lights on). ( A ) In the late afternoon, occludin (green) was uniformly primarily localized to the lateral membranes of the surface CE. ( B ) The same specimen as in A was labeled for the presence of claudin-4 (red), and was also uniformly primarily localized to the lateral membranes of the surface CE. ( C ) Merged green/red images from A and B demonstrate a high degree of co-localization in the surface cell CE lateral membranes, as indicated by the yellow signal. ( D ) In the late night, occludin (green) was localized to the CE surface cell lateral membranes as during the day, but the pattern of labeling was often interrupted in some clusters of cells (arrows). ( E ) The same specimen as in D was labeled for the presence of claudin-4 (red), and was also localized to the CE surface cell lateral membranes, but the pattern of labeling was also interrupted in the same clusters of cells (arrows). ( F ) Merged green/red images from D and F demonstrate a high degree of co-localization in the surface cell CE lateral membranes, as indicated by the yellow signal, with a similar level

Claudin 4 Antibody in Immunohistochemistry (IHC) — Figure 6 The murine jejunal tight junctional network is affected by DXR exposure. ( A ) RT-qPCR of tight junctional genes in jejunal crypt epithelium isolated from control tissues and tissues collected 24 h after DXR from conventionally raised mice ( n = 3/group). All data is presented as fold change normalized to Actb . Student's t test; *p < 0.05, **p < 0.01. ( B ) Representative images of immunofluorescent staining of tight junction associated proteins Claudin-2 (Cldn2), Claudin-4 (Cldn4), and Zona Occludens-1 (ZO-1) in control tissues (Cntrl) and tissues collected 24 h after DXR (24 h) from conventionally raised mice. Images are representative of n = 3 per time point. Scale bar 20 mum.

Claudin 4 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 1 Claudin-4 present at the basal surface of the STB layer diminished in ZIKV-infected placentae. ( a ) Frozen sections of human placentae derived from women infected or not (control) with ZIKV were processed for immunofluorescence with a rabbit antibody against claudin-4 (Cl-4) and a mouse antibody anti cytokeratin 18 (K18). DNA of nuclei was stained with DAPI. Apical surface of STB cell layer (arrow); basolateral surface of STB cell layer (arrowhead); intervillous space (asterisk). Bar, 50 mum; magnification bar, 25 mum. ( b ) Measurements of mean fluorescence intensity of the trophoblast layer were done on three independent images from each condition. Since the variances were equal among the two groups, we applied a Student's t -test. To include effect size estimation, since both groups have similar standard deviations, we used Cohen's d . The three values obtained per donor are represented by dots with the same color. ** p = 0.0054, Cohen's d = 3 indicating that 99.9% of the values from the ZIKV group are below the mean value of the control group. ( c ) Representative Western blot of three independent experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was employed as loading control. ( d ) Densitometric analysis of Western blots. The three values obtained per donor are represented by dots with the same color. ** p = 0.00092 Cohen's d = 2.67 indicating that 99.9% of the values from the ZIKV group are below the mean value of the control group.

Product Details

36-4800

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 29 publications 29 publications
Immunohistochemistry (IHC)	-	View 24 publications 24 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 4 publications 4 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	Assay-dependent	View 17 publications 17 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	0.1-1 µg/mL	-
Immunoprecipitation (IP)	1-3 µg/mL	-
Miscellaneous PubMed (Misc)	-	View 5 publications 5 publications

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Dog, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rat, Sheep
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Clone	ZMD.306
Immunogen	Synthetic peptide derived from the C-terminal region of mouse Claudin-4 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533262

Product Specific Information

Reactivity has been confirmed with mouse and rat kidney homogenates. On western blots a 22 kDa band is observed and a 44 kDa band representing dimerized protein may be observed.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl^- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

B. adolescentis ameliorates chronic colitis by regulating Treg/Th2 response and gut microbiota remodeling.

Gut microbes

Fan L,Qi Y,Qu S,Chen X,Li A,Hendi M,Xu C,Wang L,Hou T,Si J,Chen S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jan 18 00:00:00 EST 2022

Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern.

Communications biology

Tabariès S,Annis MG,Lazaris A,Petrillo SK,Huxham J,Abdellatif A,Palmieri V,Chabot J,Johnson RM,Van Laere S,Verhoef C,Hachem Y,Yumeen S,Meti N,Omeroglu A,Altinel G,Gao ZH,Yu ASL,Grünhagen DJ,Vermeulen P,Metrakos P,Siegel PM

36-4800 was used in Immunohistochemistry to provide evidence that Claudin-2 status in patient-derived extracellular vesicles may serve as a relevant prognostic biomarker to predict whether colorectal cancer patients have developed replacement type liver metastases.

Wed Jun 02 00:00:00 EDT 2021

Tight Junction Protein Claudin-7 Is Essential for Intestinal Epithelial Stem Cell Self-Renewal and Differentiation.

Cellular and molecular gastroenterology and hepatology

Xing T,Benderman LJ,Sabu S,Parker J,Yang J,Lu Q,Ding L,Chen YH

36-4800 was used in Western Blotting to show that Cldn7 is indispensable in controlling Wnt/β-catenin signaling-dependent intestinal epithelial stem cell survival, self-renewal, and cell differentiation.

Mon May 10 00:00:00 EDT 2021

Quercetin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes.

Nutrients

Jia H,Zhang Y,Si X,Jin Y,Jiang D,Dai Z,Wu Z

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jan 26 00:00:00 EST 2021

Doxorubicin increases permeability of murine small intestinal epithelium and cultured T84 monolayers.

Scientific reports

Cray P,Sheahan BJ,Cortes JE,Dekaney CM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Dec 08 00:00:00 EST 2020

Doxorubicin increases permeability of murine small intestinal epithelium and cultured T84 monolayers.

Scientific reports

Cray P,Sheahan BJ,Cortes JE,Dekaney CM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Dec 08 00:00:00 EST 2020

E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions.

International journal of oncology

Uc PY,Miranda J,Raya-Sandino A,Alarcón L,Roldán ML,Ocadiz-Delgado R,Cortés-Malagón EM,Chávez-Munguía B,Ramírez G,Asomoza R,Shoshani L,Gariglio P,González-Mariscal L

36-4800 was used in Western Blotting to investigate the role of human papillomavirus (HPV) 16 E7 oncoprotein on the sealing of TJs, and also the expression level of claudins in mouse cervix and in epithelial Madin‚Darby Canine Kidney (MDCK) cells.

Thu Oct 01 00:00:00 EDT 2020

Impact of Sensitization and Inflammation on the Interaction of Mast Cells With the Intestinal Epithelium in Rats.

Frontiers in physiology

Becker J,Ott D,Diener M

36-4800 was used in Immunohistochemistry-immunofluorescence to study the impact of sensitisation and inflammation on the interaction of mast cells with the intestinal epithelium in rats.

Wed Sep 30 00:00:00 EDT 2020

Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration.

Cell stem cell

Choi J,Park JE,Tsagkogeorga G,Yanagita M,Koo BK,Han N,Lee JH

36-4800 was used in Immunohistochemistry (Paraffin) to map the trajectories of alveolar-lineage cells during injury repair and lung regeneration by single-cell RNA sequencing in combination with in vivo lineage tracing and organoid models.

Thu Sep 03 00:00:00 EDT 2020

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Nature cell biology

Kobayashi Y,Tata A,Konkimalla A,Katsura H,Lee RF,Ou J,Banovich NE,Kropski JA,Tata PR

36-4800 was used in Immunocytochemistry-Immunofluorescence to implicate a transient state associated with senescence in normal epithelial tissue repair and its abnormal persistence in disease conditions.

Sat Aug 01 00:00:00 EDT 2020

NOTCH1 signaling establishes the medullary thymic epithelial cell progenitor pool during mouse fetal development.

Development (Cambridge, England)

Li J,Gordon J,Chen ELY,Xiao S,Wu L,Zúñiga-Pflücker JC,Manley NR

36-4800 was used in Immunocytochemistry to provide strong evidence for a requirement for notch signaling in specification of the mTEC lineage.

Mon Jun 22 00:00:00 EDT 2020

Hyaluronan 35 kDa enhances epithelial barrier function and protects against the development of murine necrotizing enterocolitis.

Pediatric research

Gunasekaran A,Eckert J,Burge K,Zheng W,Yu Z,Kessler S,de la Motte C,Chaaban H

36-4800 was used in Immunohistochemistry to investigate the effects of HA 35 kDa on a NEC-like murine model.

Mon Jun 01 00:00:00 EDT 2020

Resolvin E1 is a pro-repair molecule that promotes intestinal epithelial wound healing.

Proceedings of the National Academy of Sciences of the United States of America

Quiros M,Feier D,Birkl D,Agarwal R,Zhou DW,García AJ,Parkos CA,Nusrat A

36-4800 was used in Western Blotting to investigate the role for RvE1 in regulating intestinal epithelial cell migration and proliferation during mucosal wound repair.

Tue Apr 28 00:00:00 EDT 2020

Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection.

Cellular and molecular gastroenterology and hepatology

Suzuki K,Sentani K,Tanaka H,Yano T,Suzuki K,Oshima M,Yasui W,Tamura A,Tsukita S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 08 00:00:00 EDT 2020

Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection.

Cellular and molecular gastroenterology and hepatology

Suzuki K,Sentani K,Tanaka H,Yano T,Suzuki K,Oshima M,Yasui W,Tamura A,Tsukita S

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 08 00:00:00 EDT 2020

Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro.

Toxicology in vitro : an international journal published in association with BIBRA

Kobayashi K,Tsugami Y,Suzuki N,Suzuki T,Nishimura T

36-4800 was used in Immunohistochemistry to investigate whether nicotine directly affects milk production in lactating mammary epithelial cells.

Sun Mar 01 00:00:00 EST 2020

Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota.

Gut microbes

Jang YJ,Kim WK,Han DH,Lee K,Ko G

36-4800 was used in Western Blotting to evaluate immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans.

Mon Feb 10 00:00:00 EST 2020

The Differentiation-Associated Keratinocyte Protein Cornifelin Contributes to Cell-Cell Adhesion of Epidermal and Mucosal Keratinocytes.

The Journal of investigative dermatology

Wagner T,Beer L,Gschwandtner M,Eckhart L,Kalinina P,Laggner M,Ellinger A,Gruber R,Kuchler U,Golabi B,Tschachler E,Mildner M

36-4800 was used in Western Blotting to indicate that CNFN is a structural component of the cell adhesion system of differentiated KCs in both epidermis and oral mucosa.

Fri Nov 01 00:00:00 EDT 2019

Effects of Claudin-1 on the Action of Clostridium perfringens Enterotoxin in Caco-2 Cells.

Toxins

Mehdizadeh Gohari I,Li J,Navarro M,Uzal F,McClane B

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 09 00:00:00 EDT 2019

Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells.

International journal of molecular sciences

Gamero-Estevez E,Andonian S,Jean-Claude B,Gupta I,Ryan AK

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 02 00:00:00 EDT 2019

Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells.

International journal of molecular sciences

Gamero-Estevez E,Andonian S,Jean-Claude B,Gupta I,Ryan AK

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Oct 02 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Miranda J,Martín-Tapia D,Valdespino-Vázquez Y,Alarcón L,Espejel-Nuñez A,Guzmán-Huerta M,Muñoz-Medina JE,Shibayama M,Chávez-Munguía B,Estrada-Gutiérrez G,Lievano S,Ludert JE,González-Mariscal L

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Sun Sep 29 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Sun Sep 29 00:00:00 EDT 2019

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability.

Cells

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Sun Sep 29 00:00:00 EDT 2019

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Journal of the peripheral nervous system : JPNS

Ouyang X,Dong C,Ubogu EE

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

The kidney anion exchanger 1 affects tight junction properties via claudin-4.

Scientific reports

Lashhab R,Rumley AC,Arutyunov D,Rizvi M,You C,Dimke H,Touret N,Zimmermann R,Jung M,Chen XZ,Alexander T,Cordat E

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Thu Feb 28 00:00:00 EST 2019

Immunoexpression of claudins 4 and 7 among invasive breast carcinoma subtypes: A large diagnostic study using tissue microarray.

Molecular and clinical oncology

Logullo AF,Pasini FS,Nonogaki S,Rocha RM,Soares FA,Brentani MM

36-4800 was used in Immunohistochemistry-immunofluorescence to study whether the immunoexpression of claudins 4 and 7 in tissue microarray is associated with survival and prognosis in luminal A, HER-2 and triple-negative molecular breast cancer subtypes.

Mon Oct 01 00:00:00 EDT 2018

The Human Blood-Nerve Barrier Transcriptome.

Scientific reports

Palladino SP,Helton ES,Jain P,Dong C,Crowley MR,Crossman DK,Ubogu EE

36-4800 was used in Immunocytochemistry-immunoflourescence to sequence the human blood-nerve barrier transcriptome using confluent P3 and P8 pHEndEC monolayers and normal adult laser-capture microdissected endoneurial microvessels.

Tue Dec 12 00:00:00 EST 2017

Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.

Oncology letters

Shimada H,Satohisa S,Kohno T,Konno T,Takano KI,Takahashi S,Hatakeyama T,Arimoto C,Saito T,Kojima T

36-4800 was used in Western Blotting to indicate that the downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion of human endometrial cancer via claudin-1 mediation of matrix metalloproteinases.

Fri Dec 01 00:00:00 EST 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jul 11 00:00:00 EDT 2017

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Scientific reports

Ahmad R,Rah B,Bastola D,Dhawan P,Singh AB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Tue Jul 11 00:00:00 EDT 2017

Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium.

The EMBO journal

Miyoshi H,VanDussen KL,Malvin NP,Ryu SH,Wang Y,Sonnek NM,Lai CW,Stappenbeck TS

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Wed Jan 04 00:00:00 EST 2017

Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium.

The EMBO journal

Miyoshi H,VanDussen KL,Malvin NP,Ryu SH,Wang Y,Sonnek NM,Lai CW,Stappenbeck TS

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunocytochemistry

Wed Jan 04 00:00:00 EST 2017

Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.

Journal of veterinary science

Lee B,Kang HY,Lee DO,Ahn C,Jeung EB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Fri Dec 30 00:00:00 EST 2016

Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.

Journal of veterinary science

Lee B,Kang HY,Lee DO,Ahn C,Jeung EB

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Fri Dec 30 00:00:00 EST 2016

Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with β-casein expression in mammary epithelial cells.

Biochimica et biophysica acta

Kobayashi K,Tsugami Y,Matsunaga K,Oyama S,Kuki C,Kumura H

36-4800 was used in immunohistochemistry - paraffin section to characterize induction of lactation-specific tight junctions concurrent with beta-casein expression in mammary epithelial cells due to prolactin and glucocorticoid signaling

Mon Aug 01 00:00:00 EDT 2016

Connexins, E-cadherin, Claudin-7 and β-catenin transiently form junctional nexuses during the post-natal mammary gland development.

Developmental biology

Dianati E,Poiraud J,Weber-Ouellette A,Plante I

36-4800 was used in immunohistochemistry - frozen section and western blot to assess the transiently formed junctional nexuses during post-natal mammary gland development by E-cadherins, connexins, beta-catenin, and claudin-7

Mon Aug 01 00:00:00 EDT 2016

Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.

PloS one

Fredriksson K,Van Itallie CM,Aponte A,Gucek M,Tietgens AJ,Anderson JM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Fri Apr 29 00:00:00 EDT 2016

Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.

PloS one

Fredriksson K,Van Itallie CM,Aponte A,Gucek M,Tietgens AJ,Anderson JM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Fri Apr 29 00:00:00 EDT 2016

Diurnal variation of tight junction integrity associates inversely with matrix metalloproteinase expression in Xenopus laevis corneal epithelium: implications for circadian regulation of homeostatic surface cell desquamation.

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

PloS one

Wiechmann AF,Ceresa BP,Howard EW

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Tue Dec 22 00:00:00 EST 2015

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

The Journal of cell biology

Gao X,Bali AS,Randell SH,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Nov 09 00:00:00 EST 2015

GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

The Journal of cell biology

Gao X,Bali AS,Randell SH,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Mon Nov 09 00:00:00 EST 2015

Diverse regulation of claudin-1 and claudin-4 in atopic dermatitis.

The American journal of pathology

Gruber R,Börnchen C,Rose K,Daubmann A,Volksdorf T,Wladykowski E,Vidal-Y-Sy S,Peters EM,Danso M,Bouwstra JA,Hennies HC,Moll I,Schmuth M,Brandner JM

36-4800 was used in immunohistochemistry (paraffin) to study how Cldn-1 and Cldn-4 are altered in atopic dermatitis.

Thu Oct 01 00:00:00 EDT 2015

A non-tight junction function of claudin-7-Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment.

Molecular cancer

Lu Z,Kim DH,Fan J,Lu Q,Verbanac K,Ding L,Renegar R,Chen YH

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Wed Jun 17 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Mon Jun 15 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Western Blot

Mon Jun 15 00:00:00 EDT 2015

Mode of action of claudin peptidomimetics in the transient opening of cellular tight junction barriers.

Biomaterials

Staat C,Coisne C,Dabrowski S,Stamatovic SM,Andjelkovic AV,Wolburg H,Engelhardt B,Blasig IE

36-4800 was used in immunocytochemistry to investigate the role of claudin-1/claudin-5 in epithelial/endothelial barriers

Mon Jun 01 00:00:00 EDT 2015

NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation.

Tissue barriers

Ward C,Schlingmann B,Stecenko AA,Guidot DM,Koval M

36-4800 was used in Western Blotting to suggest NF-κB regulates alveolar barrier function and that constitutive NF-κB function is required for alveolar epithelial tight junction integrity.

Fri Apr 03 00:00:00 EDT 2015

Impaired intestinal mucosal barrier upon ischemia-reperfusion: "patching holes in the shield with a simple surgical method".

BioMed research international

Rosero O,Ónody P,Kovács T,Molnár D,Lotz G,Tóth S,Turóczi Z,Fülöp A,Garbaisz D,Harsányi L,Szijártó A

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Thu Feb 12 00:00:00 EST 2015

Impaired intestinal mucosal barrier upon ischemia-reperfusion: "patching holes in the shield with a simple surgical method".

BioMed research international

Rosero O,Ónody P,Kovács T,Molnár D,Lotz G,Tóth S,Turóczi Z,Fülöp A,Garbaisz D,Harsányi L,Szijártó A

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Thu Feb 12 00:00:00 EST 2015

EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells.

Journal of cellular physiology

García-Hernández V,Flores-Maldonado C,Rincon-Heredia R,Verdejo-Torres O,Bonilla-Delgado J,Meneses-Morales I,Gariglio P,Contreras RG

36-4800 was used in immunocytochemistry and western blot to investigate the effect of EGF on claudin-2 and -4 expression in MDCK cells.

Thu Jan 01 00:00:00 EST 2015

Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice.

Cell death & disease

Ruan L,Zhang Z,Mu L,Burnley P,Wang L,Coder B,Zhuge Q,Su DM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 09 00:00:00 EDT 2014

Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice.

Cell death & disease

Ruan L,Zhang Z,Mu L,Burnley P,Wang L,Coder B,Zhuge Q,Su DM

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 09 00:00:00 EDT 2014

Enteral supplementation of bovine lactoferrin improves gut barrier function in rats after massive bowel resection.

The British journal of nutrition

Wu J,Chen J,Wu W,Shi J,Zhong Y,van Tol EA,Tang Q,Cai W

36-4800 was used in western blot to assess the effect of enteral bovine lactoferrin supplementation on intestinal adaptation and barrier function in a rat model of short bowel syndrome.

Thu Aug 28 00:00:00 EDT 2014

Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.

Development (Cambridge, England)

Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER

36-4800 was used in immunohistochemistry - paraffin section to assess the contribution of Tbx1 to thymus and parathyroid development

Fri Aug 01 00:00:00 EDT 2014

Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.

Development (Cambridge, England)

Castillo D,Seidel K,Salcedo E,Ahn C,de Sauvage FJ,Klein OD,Barlow LA

36-4800 was used in immunohistochemistry - frozen section to examine the effects of sonic hedgehog on the lingual epithelium of adult mice

Fri Aug 01 00:00:00 EDT 2014

Role of the p63-FoxN1 regulatory axis in thymic epithelial cell homeostasis during aging.

Cell death & disease

Burnley P,Rahman M,Wang H,Zhang Z,Sun X,Zhuge Q,Su DM

36-4800 was used in immunohistochemistry (frozen) to assess the contributions of the p63 gene and FoxN1 to thymic epithelial cell homeostasis during thymic aging.

Thu Nov 21 00:00:00 EST 2013

Gastroesophageal reflux activates the NF-κB pathway and impairs esophageal barrier function in mice.

American journal of physiology. Gastrointestinal and liver physiology

Fang Y,Chen H,Hu Y,Djukic Z,Tevebaugh W,Shaheen NJ,Orlando RC,Hu J,Chen X

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Mon Jul 01 00:00:00 EDT 2013

Gastroesophageal reflux activates the NF-κB pathway and impairs esophageal barrier function in mice.

American journal of physiology. Gastrointestinal and liver physiology

Fang Y,Chen H,Hu Y,Djukic Z,Tevebaugh W,Shaheen NJ,Orlando RC,Hu J,Chen X

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Mon Jul 01 00:00:00 EDT 2013

Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].

Proceedings of the National Academy of Sciences of the United States of America

Gao X,Vockley CM,Pauli F,Newberry KM,Xue Y,Randell SH,Reddy TE,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Jun 04 00:00:00 EDT 2013

Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].

Proceedings of the National Academy of Sciences of the United States of America

Gao X,Vockley CM,Pauli F,Newberry KM,Xue Y,Randell SH,Reddy TE,Hogan BL

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Tue Jun 04 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed May 01 00:00:00 EDT 2013

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Histochemistry and cell biology

Kratzer I,Vasiljevic A,Rey C,Fevre-Montange M,Saunders N,Strazielle N,Ghersi-Egea JF

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Aging and disease

Xia J,Wang H,Guo J,Zhang Z,Coder B,Su DM

36-4800 was used in immunohistochemistry to test if disruption of the steady-state thymic medulla results in the increased autoimmunity associated with age.

Fri Jun 01 00:00:00 EDT 2012

CD44 regulates tight-junction assembly and barrier function.

The Journal of investigative dermatology

Kirschner N,Haftek M,Niessen CM,Behne MJ,Furuse M,Moll I,Brandner JM

Fri Apr 01 00:00:00 EDT 2011

Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-ζ and PP2A pathway.

American journal of physiology. Lung cellular and molecular physiology

Caraballo JC,Yshii C,Butti ML,Westphal W,Borcherding JA,Allamargot C,Comellas AP

Fri Apr 01 00:00:00 EDT 2011

Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium.

Journal of molecular medicine (Berlin, Germany)

Guo J,Rahman M,Cheng L,Zhang S,Tvinnereim A,Su DM

36-4800 was used in flow cytometry and immunohistochemistry - paraffin section to elucidate how FoxN1 contributes to cyst formation and thyroid development

Tue Mar 01 00:00:00 EST 2011

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy.

Reproduction (Cambridge, England)

Bailey DW,Dunlap KA,Frank JW,Erikson DW,White BG,Bazer FW,Burghardt RC,Johnson GA

36-4800 was used in Immunofluorescence in immunohistochemistry on frozen tissues to show that progesterone affects luminal epithelium, glandular epithelium (GE), and vascular development and histoarchitecture.

Fri Oct 01 00:00:00 EDT 2010

Cell polarity regulator PARD6B is essential for trophectoderm formation in the preimplantation mouse embryo.

Biology of reproduction

Alarcon VB

36-4800 was used in immunocytochemistry to elucidate the role of partitioning defective genes in trophectoderm differentiation and blastocyst formation

Wed Sep 01 00:00:00 EDT 2010

Postnatal tissue-specific disruption of transcription factor FoxN1 triggers acute thymic atrophy.

The Journal of biological chemistry

Cheng L,Guo J,Sun L,Fu J,Barnes PF,Metzger D,Chambon P,Oshima RG,Amagai T,Su DM

36-4800 was used in immunohistochemistry to investigate the postnatal contribution of FoxN1 to thymic maintenance

Fri Feb 19 00:00:00 EST 2010

Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.

Gastroenterology

Chang TL,Ito K,Ko TK,Liu Q,Salto-Tellez M,Yeoh KG,Fukamachi H,Ito Y

Fri Jan 01 00:00:00 EST 2010

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 29 00:00:00 EDT 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunofluorescence

Thu Oct 29 00:00:00 EDT 2009

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.

Molecular biology of the cell

den Elzen N,Buttery CV,Maddugoda MP,Ren G,Yap AS

Sat Aug 01 00:00:00 EDT 2009

Altered gene expression in breast cancer liver metastases.

International journal of cancer

Erin N,Wang N,Xin P,Bui V,Weisz J,Barkan GA,Zhao W,Shearer D,Clawson GA

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2009

Altered gene expression in breast cancer liver metastases.

International journal of cancer

Erin N,Wang N,Xin P,Bui V,Weisz J,Barkan GA,Zhao W,Shearer D,Clawson GA

Published figure using Claudin 4 polyclonal antibody (Product # 36-4800) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2009

Phosphorylation of claudin-4 is required for tight junction formation in a human keratinocyte cell line.

Experimental cell research

Aono S,Hirai Y

36-4800 was used in immunocytochemistry and western blot to suggest that aPKC regulates tight junction formation through the phosphorylation of claudin-4

Sat Nov 01 00:00:00 EDT 2008

Tight and adherens junctions in the ovine uterus: differential regulation by pregnancy and progesterone.

Endocrinology

Satterfield MC,Dunlap KA,Hayashi K,Burghardt RC,Spencer TE,Bazer FW

36-4800 was used in Immunohistochemistry to identify changes in junctional complexes in the ovine endometrium that regulate paracellular trafficking of water, ions, and other molecules, and the secretory capacity of the uterine epithelia.