Invitrogen

Claudin 4 Polyclonal Antibody (ZMD.306)

Product Image Gallery

Claudin 4 Antibody — Figure 1. Examples of CD24, CD44 and claudin 4 and 7 immunostaining in mammary invasive carcinomas (scale bar, 100 um). (A) Claudin-negative; (B) CD24-negative; (C) CD44-negative; (D) claudin 4 2+; (E) claudin 7 2+; (F) CD24-positive; (G) claudin 4 3+; (H) claudin 7 3+; (I) CD44-positive.

Claudin 4 Antibody in Western Blot (WB) — Western blot analysis of (left) mouse and (right rat kidney homogenates using Rabbit anti-Claudin-4 Polyclonal Antibody (Product # 36-4800).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

0.1-1 µg/mL

Immunofluorescence (IF)

Assay Dependent

Immunoprecipitation (IP)

1-3 µg/mL

Western Blot (WB)

1-3 µg/mL

Published Applications

Immunofluorescence (IF)

See 11 publications below

Immunohistochemistry (IHC)

See 12 publications below

Immunocytochemistry (ICC)

See 5 publications below

Western Blot (WB)

See 14 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 3 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 3 publications below

Miscellaneous PubMed (MISC)

See 6 publications below

Flow Cytometry (Flow)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Dog, Fish, Hamster, Human, Mouse, Non-human primate, Plant, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

ZMD.306

Immunogen

Synthetic peptide derived from the C-terminal region of mouse Claudin-4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533262

Product Specific Information

Reactivity has been confirmed with mouse and rat kidney homogenates. On western blots a 22 kDa band is observed and a 44 kDa band representing dimerized protein may be observed.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl^- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.