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Immunofluorescence: Claudin-5, Mouse Monoclonal Antibody - Alexa Fluor® 488: (Prod # 352588) Human Caco-2 cells stained with Claudin-5, Mouse Monoclonal Antibody - Alexa Fluor® 488 (Prod # 352588). DNA is counter stained with blue Hoechst 33258 (Cat. No H3569). For high resolution colored figure, please visit the product page online. (Prod # 352588).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide derived from the mouse Claudin-5 protein|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Claudin proteins are a family of proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Real-time estimation of paracellular permeability of cerebral endothelial cells by capacitance sensor array.
352588 was used in immunocytochemistry to evaluate capacitance sensor array for the estimation of paracellular permeability of cerebral endothelial cells
|Hyun Jo D,Lee R,Hyoung Kim J,Oh Jun H,Geol Lee T,Hun Kim J||Scientific reports (5:null)||2015|
The role of the hypoxia response in shaping retinal vascular development in the absence of Norrin/Frizzled4 signaling.
352588 was used in immunohistochemistry to investigate the relationship between hypoxia response and Norrin/Frizzled4 signaling during retinal vascular development
|Rattner A,Wang Y,Zhou Y,Williams J,Nathans J||Investigative ophthalmology & visual science (55:8614)||2014|
EndMT contributes to the onset and progression of cerebral cavernous malformations.
352588 was used in western blot to study the role of endothelial-to-mesenchymal transition in the onset and progression of cerebral cavernous malformations
|Maddaluno L,Rudini N,Cuttano R,Bravi L,Giampietro C,Corada M,Ferrarini L,Orsenigo F,Papa E,Boulday G,Tournier-Lasserve E,Chapon F,Richichi C,Retta SF,Lampugnani MG,Dejana E||Nature (498:492)||2013|
Norrin/Frizzled4 signaling in retinal vascular development and blood brain barrier plasticity.
352588 was used in immunohistochemistry to investigate the role of Norrin in retinal vascular development.
|Wang Y,Rattner A,Zhou Y,Williams J,Smallwood PM,Nathans J||Cell (151:1332)||2012|
AWAL; Brain endothelial cell clone 1 protein; Claudin-5; CPETRL1; Lung-specific membrane protein; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome
AI854493; AWAL; BEC1; CLDN5; CPETRL1; MBEC1; TMVCF