Immunofluorescence analysis of Claudin-7 was done on 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin-7 (5D10F3) Mouse Monoclonal Antibody (374800) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cell junction localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human claudin-7 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.
37-4800 was used in western blot to investigate the effect of homoharringtonine on intestinal epithelial permeability and its mechanism
|Watari A,Hashegawa M,Yagi K,Kondoh M||European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (89:232)||2015|
Rab25 regulates integrin expression in polarized colonic epithelial cells.
37-4800 was used in western blot to identify elements of intestinal polarity regulated by Rab25.
|Krishnan M,Lapierre LA,Knowles BC,Goldenring JR||Molecular biology of the cell (24:818)||2013|
Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis).
37-4800 was used in western blot to develop an in vitro model of gelatinous drop-like corneal dystrophy using a new cell line deficient in tumor-associated calcium signal transducer 2.
|Kinoshita S,Kawasaki S,Kitazawa K,Shinomiya K||Transactions of the American Ophthalmological Society (110:166)||2012|
Franceschetti hereditary recurrent corneal erosion.
37-4800 was used in immunohistochemistry to identify and describe Franceschetti corneal dystrophy.
|Lisch W,Bron AJ,Munier FL,Schorderet DF,Tiab L,Lange C,Saikia P,Reinhard T,Weiss JS,Gundlach E,Pleyer U,Lisch C,Auw-Haedrich C||American journal of ophthalmology (153:1073)||2012|
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
37-4800 was used in immunocytochemistry, immunohistochemistry, and western blot to determine the relationship between the TACSTD2 gene and epithelial barrier function
|Nakatsukasa M,Kawasaki S,Yamasaki K,Fukuoka H,Matsuda A,Tsujikawa M,Tanioka H,Nagata-Takaoka M,Hamuro J,Kinoshita S||The American journal of pathology (177:1344)||2010|
Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma.
37-4800 was used in immunohistochemistry (paraffin) to examine the complexity of the invasive front of colon carcinoma.
|Victoria HS,Henrik E,Lennart B,Lennart F||International journal of biomedical science : IJBS (6:96)||2010|
Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells.
37-4800 was used in immunocytochemistry to investigate the contribution of CA125/MUC16 to metastasis of epithelial ovarian cancer.
|Comamala M,Pinard M,Thériault C,Matte I,Albert A,Boivin M,Beaudin J,Piché A,Rancourt C||British journal of cancer (104:989)||2011|
|Not Applicable||Not Cited||
Claudin-7 expression in human epithelial ovarian cancer.
37-4800 was used in immunocytochemistry and immunohistochemistry - paraffin section to explore the use of Claudin-7 as a biomarker for ovarian adenocarcinoma
|Tassi RA,Bignotti E,Falchetti M,Ravanini M,Calza S,Ravaggi A,Bandiera E,Facchetti F,Pecorelli S,Santin AD||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (18:1262)||2009|
Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease.
37-4800 was used in immunocytochemistry to measure Claudin-7 expression in smears from breast carcinomas and correlate expression with grading, nodal status, locoregional and distant metastases, and the cellular cohesion
|Sauer T,Pedersen MK,Ebeltoft K,Naess O||Cytopathology : official journal of the British Society for Clinical Cytology (16:193)||2005|
Cluster analysis of immunohistochemical profiles delineates CK7, vimentin, S100A1 and C-kit (CD117) as an optimal panel in the differential diagnosis of renal oncocytoma from its mimics.
37-4800 was used in immunohistochemistry - paraffin section to discuss how to distinguish renal oncocytoma from the eosinophilic variant of chromophobe, and papillary and clear cell renal cell carcinoma.
|Carvalho JC,Wasco MJ,Kunju LP,Thomas DG,Shah RB||Histopathology (58:169)||2011|
|Human||Not Cited||Differential expression of claudin tight junction proteins in the human cortical nephron.||Kirk A,Campbell S,Bass P,Mason J,Collins J||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (25:2107)||2010|