Immunofluorescent analysis of Cleavage stimulation factor 2 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Cleavage stimulation factor 2 polyclonal antibody (Product # PA5-30245) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 181 of Human Cleavage stimulation factor 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30245 targets Cleavage stimulation factor 2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30245 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 181 of Human Cleavage stimulation factor 2.
This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.
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