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Proteintech
Cleaved Caspase 3 p17 Monoclonal Antibody (2F7B8)
FIGURE: 1 / 18
Cleaved Caspase 3 p17 Antibody (68773-1-IG) in ICC/IF
Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8) at dilution of 1:1,000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).
Product Details
68773-1-IG
Applications
Tested Dilution
Publications
Immunoprecipitation (IP)
0.5-4.0 µg for 1.0-3.0 mg of total protein lysate
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F7B8
Immunogen
Peptide
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Tiso et al. (1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apopain; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Cleaved Caspase 3; CPP-32; CPP32beta; Cysteine protease CPP32; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein
Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213
Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402
protease binding
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
phospholipase A2 activator activity
protein complex binding
cysteine-type endopeptidase activity involved in apoptotic process
cysteine-type endopeptidase activity involved in execution phase of apoptosis
cysteine-type peptidase activity
hydrolase activity
protease
response to hypoxia
B cell homeostasis
apoptotic DNA fragmentation
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
heart development
sensory perception of sound
learning or memory
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
response to UV
response to glucose
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
negative regulation of B cell proliferation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
hippo signaling
wound healing
response to drug
response to hydrogen peroxide
T cell homeostasis
negative regulation of apoptotic process
response to amino acid
positive regulation of neuron apoptotic process
cell fate commitment
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of activated T cell proliferation
response to antibiotic
neurotrophin TRK receptor signaling pathway
response to glucocorticoid
neuron apoptotic process
cellular response to organic cyclic compound
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
execution phase of apoptosis
response to wounding
intracellular signal transduction
positive regulation of apoptotic process
positive regulation of catalytic activity
negative regulation of cell cycle
cellular response to organic substance
release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to oxidative stress
response to organic substance
response to metal ion
response to organic cyclic compound
regulation of apoptotic DNA fragmentation
Performance Guarantee
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