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Cofilin 2 Antibody (PA5-79037) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Cofilin 2 in U2OS cells using Cofilin 2 Polyclonal Antibody (Product # PA5-79037). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 di... View More
Product Details
PA5-79037
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2 (121-153aa KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746153
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell lysates, human HUVEC whole cell lysates, human HepG2 whole cell lysates, human Hela whole cell lysates, rat skeletal muscle tissue lysates, rat RH35 whole cell lysates, rat liver tissue lysates, mouse skeletal muscle tissue lysates, mouse liver tissue lysates. ICC/IF: U2OS cells. Flow: Raji cells.
Target Information
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COF2; cofilin 2 (muscle); Cofilin, muscle isoform; Cofilin-2; HGNC:1875; nemaline myopathy type 7
Gene Aliases: CFL2; NEM7
UniProt ID: (Human) Q9Y281, (Mouse) P45591
Entrez Gene ID: (Human) 1073, (Rat) 366624, (Mouse) 12632
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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