|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli (His-cofilin)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is Jurkat cells.
Cofilin belongs to a family of related proteins with similar biochemical activities called the actin depolymerizing factor (ADF)/cofilin familyADF/cofilins play an important role in cytokinesis , endocytosis, and in the development of all embryonic tissues.Cofilin is a small ubiquitous protein (~19 kDa) that is able to bind both G-actin (monomeric) and F-actin (filamentous actin). Cofilin promotes the regeneration of actin filaments by severing pre-existing filaments. LIMK or TESK play a central role in the regulation of the actin cytoskeleton by phosphorylating cofilin on Ser3. Phosphorylation of cofilin at Ser3 inhibits its ability to bind to actin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.