Western blot analysis of Coilin using anti-Coilin monoclonal antibody (MA5-17260) on (Lane 1) HeLa Cell lysate, (Lane 2) SK-N-MC Cell lysate, (Lane 3) K562 Cell lysate, (Lane 4) A431 Cell lysate, (Lane 5) HepG2 Cell lysate, (Lane 6) SK-BR3 Cell lysate and (Lane 7) Ramos Cell lysate.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human protein purified from E.coli (His-Coilin)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cell lysate.
Coilin is a component of the nuclear Cajar (coiled) bodies (CBs) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. Human coilin is a 576-amino acid protein found enriched in CBs, but is also found in large amounts in the nucleoplasm. Coilin is a constitutive phosphoprotein that is hyperphosphorylated during mitosis and it is thought that hyperphosphorylation triggers CB disassembly during cell replication. Self-interaction and localization have been shown to depend on the phosphorylation state of coilin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.