Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Coilin using anti-Coilin monoclonal antibody (MA5-17260) on (Lane 1) HeLa Cell lysate, (Lane 2) SK-N-MC Cell lysate, (Lane 3) K562 Cell lysate, (Lane 4) A431 Cell lysate, (Lane 5) HepG2 Cell lysate, (Lane 6) SK-BR3 Cell lysate and (Lane 7) Ramos Cell lysate.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human protein purified from E.coli (His-Coilin)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cell lysate.
Coilin is a component of the nuclear Cajar (coiled) bodies (CBs) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. Human coilin is a 576-amino acid protein found enriched in CBs, but is also found in large amounts in the nucleoplasm. Coilin is a constitutive phosphoprotein that is hyperphosphorylated during mitosis and it is thought that hyperphosphorylation triggers CB disassembly during cell replication. Self-interaction and localization have been shown to depend on the phosphorylation state of coilin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CLN80; coilin p80; p80
CLN80; COIL; p80-coilin