Immunofluorescent analysis of Coilin in HeLa cells, fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether, using a Coilin monoclonal antibody (Product # MA1-25322) at a dilution of 10 µg/mL followed by detection using a Goat anti-Mouse IgG, Cy3 conjugate. Nuclear were stained using DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment corresponding to the 389 residue, C-terminal portion of human Coilin.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25322 detects Coilin from human samples. MA1-25322 will not react with mouse.
MA1-25322 has been successfully used in Western blot procedures.
The MA1-25322 immunogen is a recombinant fragment corresponding to the 389 residue, C-terminal portion of human Coilin.
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
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