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|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen type I purified from rat skin|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Radioimmune Assays (RIA)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27397 detects Collagen I from rat samples. PA1-27397 will not react with Collagen type I from human or chicken sources, or with mouse Collagen type I when tested by RIA. It is also known that this antibody is not reactive with rat Collagen type III or Elastin. Cross-reacts with mouse collagen type 1 when tested by ELISA and IHC but does not cross react when tested by RIA.
PA1-27397 has been successfully used in immunofluorescence, ELISA and radioimmune assay procedures.
The PA1-27397 immunogen is Collagen type I purified from rat skin.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This preparation results in a native conformation of the protein. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the gene encoding collagen 1 are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alpha-1 type I collagen; Collagen 1; collagen alpha-1(I) chain; collagen, type 1, alpha 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; Type I Collagen; type I-alpha 1 collagen