Immunohistochemistry was performed on paraffin-embedded mouse gastric tissue sections. To expose target antigens, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 9 minutes in the microwave. Tissues were fixed in 4% paraformaldehyde, blocked in a buffer containing 0.1% gelatin, 0.8% BSA and 0.002% Tween-80 for 2 hours at room temperature and then probed with a Collagen I polyclonal antibody (Product # PA1-85319) at a dilution of 1:150 overnight at 4C. Tissues were washed extensively with PBS and detection was performed using a fluorescently-conjugated goat anti-rabbit IgG (green) at a dilution of 1:200. Tissues were counterstained with Blue Evans stain (red) and visualized by fluorescent microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native type I collagen from mouse skin.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/100 - 1/400|
|Immunofluorescence (IF)||1/50 - 1/100|
|Immunohistochemistry (Frozen) (IHC (F))||1:250-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250-1:1000|
|Radioimmune Assays (RIA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.
PA1-85319 was used in immunohistochemistry - paraffin section to determine how Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from initiation of polyposis
|Allaire JM,Roy SA,Ouellet C,Lemieux É,Jones C,Paquet M,Boudreau F,Perreault N||International journal of cancer (138:2700)||2016|
Effect of nebivolol treatment on atherosclerotic plaque components in apoE-knockout mice.
PA1-85319 was used in immunohistochemistry to study the effects of oral nebivolo treatment on apoE knockout mouse atherosclerotic plaque composition
|Pyka-Fosciak G,Jawien J,Gajda M,Jasek E,Litwin JA||Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (64:745)||2013|