Immunofluorescent analysis of Collagen I alpha 1 propeptide in formaldehyde-fixed, paraffin-embedded fibrotic mouse lung tissue using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379) showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. Prior to staining, antigen retrieval was performed by incubating tissues for 20 min at 90°C in 10mM sodium citrate (pH 6.0)/0.1 % Tween-20.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to human collagen 1, alpha 1 propeptide conjugated to KLH|
|Storage buffer||10mM HEPES, pH 7.5, with 150mM NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with most mammals, birds, amphibians and fish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Type I collagen is a triple helix comprised of two alpha-1 chains and one alpha-2 chain. Type I collagen is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A1 gene encoding the alpha-1 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.