|Tested species reactivity||Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Rabbit, Rat, Pig, Bovine, Sheep, Mouse, Human, Goat, Guinea pig|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A purified preparation of lathyritic type II collagen from embryonic chicken sternum|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12786 targets Collagen II in FACS, IF, IHC (P), and WB applications and shows reactivity with Bovine, Chicken, Human, mouse, and Rat samples.
The MA5-12786 immunogen is a purified preparation of lathyritic type II collagen from embryonic chicken sternum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy.
MA5-12786 was used in immunohistochemistry to study the differences in tendon-derived stem cells obtained from healthy tendons and those obtained from a rat model of tendinopathy
|Rui YF,Lui PP,Wong YM,Tan Q,Chan KM||Stem cells and development (22:1076)||2013|
Maintaining mRNA integrity during decalcification of mineralized tissues.
MA5-12786 was used in immunohistochemistry to develop a novel decalcification method that maintains mRNA integrity and aids gene expression analysis of mineralized tissues
|Belluoccio D,Rowley L,Little CB,Bateman JF||PloS one (8:null)||2013|
Quantitative polarized light microscopy of unstained mammalian cochlear sections.
MA5-12786 was used in immunohistochemistry to study unstained sections of the murine cochlear using quantitative polarized light microscopy
|Kalwani NM,Ong CA,Lysaght AC,Haward SJ,McKinley GH,Stankovic KM||Journal of biomedical optics (18:null)||2013|
FGF23 suppresses chondrocyte proliferation in the presence of soluble ¿-Klotho both in vitro and in vivo.
MA5-12786 was used in immunohistochemistry to study the suppression of chondrocyte proliferation by FGF23 in the presence of alpha-Klotho and the significance for X-linked hypophosphatemic rickets growth retardation
|Kawai M,Kinoshita S,Kimoto A,Hasegawa Y,Miyagawa K,Yamazaki M,Ohata Y,Ozono K,Michigami T||The Journal of biological chemistry (288:2414)||2013|
An in vitro study of collagen hydrogel to induce the chondrogenic differentiation of mesenchymal stem cells.
MA5-12786 was used in immunohistochemistry to study whether a collagen hydrogel can induce the in vitro chondrogenic differentiation of mesenchymal stem cells
|Zhang L,Yuan T,Guo L,Zhang X||Journal of biomedical materials research. Part A (100:2717)||2012|
Combined effect of subchondral drilling and hyaluronic acid with/without diacerein in full-thickness articular cartilage lesion in rabbits.
MA5-12786 was used in immunohistochemistry to study the osteochondral healing potential of hyaluronic acid plus diacerein
|Suwannaloet W,Laupattarakasem W,Sukon P,Ong-Chai S,Laupattarakasem P||TheScientificWorldJournal (2012:null)||2012|
Vinculin functions as regulator of chondrogenesis.
MA5-12786 was used in immunohistochemistry to study the role of the vinculin in regulating chondrogenesis in mice
|Koshimizu T,Kawai M,Kondou H,Tachikawa K,Sakai N,Ozono K,Michigami T||The Journal of biological chemistry (287:15760)||2012|
Cartilage regeneration by culturing chondrocytes in scaffolds grafted with TATVHL peptide.
MA5-12786 was used in immunohistochemistry to study cartilage regeneration by culturing chondrocytes in scaffolds grafted with TATVHL peptide
|Kuo YC,Wang CC||Colloids and surfaces. B, Biointerfaces (93:235)||2012|
The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation.
MA5-12786 was used in immunohistochemistry to study the role of Hsp47 in cartilage formation and endochondral ossification using conditional Hsp47 knockout mice
|Masago Y,Hosoya A,Kawasaki K,Kawano S,Nasu A,Toguchida J,Fujita K,Nakamura H,Kondoh G,Nagata K||Journal of cell science (125:1118)||2012|
Surface modification with peptide for enhancing chondrocyte adhesion and cartilage regeneration in porous scaffolds.
MA5-12786 was used in immunohistochemistry to develop a system for cartilage regeneration in porous scaffolds using surface peptide modification to enhance chondrocyte adhesion
|Kuo YC,Wang CC||Colloids and surfaces. B, Biointerfaces (84:63)||2011|
Heparin-conjugated scaffolds with pore structure of inverted colloidal crystals for cartilage regeneration.
MA5-12786 was used in immunohistochemistry to study cartilage regeneration using heparin-conjugated scaffolds with inverted colloidal crystal pores
|Kuo YC,Tsai YT||Colloids and surfaces. B, Biointerfaces (82:616)||2011|
Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).
MA5-12786 was used in immunohistochemistry to study the role of allogenic mesenchymal stem cell transplantation in improved healing of ischemic colon anastomosis
|Adas G,Arikan S,Karatepe O,Kemik O,Ayhan S,Karaoz E,Kamali G,Eryasar B,Ustek D||Langenbeck's archives of surgery / Deutsche Gesellschaft fu¿r Chirurgie (396:115)||2011|
Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells.
MA5-12786 was used in immunohistochemistry to study the expression of antigen presenting cell co-stimulatory molecules by pancreatic islet derived stem cells
|Karaoz E,Okçu A,Saglam O,Genc ZS,Ayhan S,Kasap M||Transplantation proceedings (42:3663)||2010|
FGF-2 abolishes the chondrogenic effect of combined BMP-6 and TGF-beta in human adipose derived stem cells.
MA5-12786 was used in immunohistochemistry to investigate the effect of basic fibroblast growth factor on chondrogenesis
|Hildner F,Peterbauer A,Wolbank S,Nürnberger S,Marlovits S,Redl H,van Griensven M,Gabriel C||Journal of biomedical materials research. Part A (94:978)||2010|
Inverted colloidal crystal scaffolds for uniform cartilage regeneration.
MA5-12786 was used in immunohistochemistry to evaluate a noval scaffolds for cartilage tissue engineering
|Kuo YC,Tsai YT||Biomacromolecules (11:731)||2010|
Collagen type distribution in the healthy human tympanic membrane.
MA5-12786 was used in immunohistochemistry to study the distribution of different collagen types in the human tympanic membrane
|Knutsson J,Bagger-Sjöbäck D,von Unge M||Otology and neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology (30:1225)||2009|
Tissue-engineered polyethylene oxide/chitosan scaffolds as potential substitutes for articular cartilage.
MA5-12786 was used in immunohistochemistry to develop articular cartilage
|Kuo YC,Hsu YR||Journal of biomedical materials research. Part A (91:277)||2009|
Articular cartilage increases transition zone regeneration in bone-tendon junction healing.
MA5-12786 was used in immunohistochemistry to study the ability of articular cartilage grafts to increase transition zone regeneration during healing of the bone-tendon junction
|Wong MW,Qin L,Lee KM,Leung KS||Clinical orthopaedics and related research (467:1092)||2009|
Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eye.
MA5-12786 was used in immunohistochemistry to evaluate the experimental methods for the handling of vitreous and retina of the rabbit
|Pfeffer BA,Bernstein SA,Bartels SP||Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fu¿r klinische und experimentelle Ophthalmologie (247:193)||2009|
Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells.
MA5-12786 was used in immunohistochemistry to study how the differentiation potential of murine bone marrow-derived stem cells is affected by donor age and cell passage number
|Kretlow JD,Jin YQ,Liu W,Zhang WJ,Hong TH,Zhou G,Baggett LS,Mikos AG,Cao Y||BMC cell biology (9:null)||2008|
In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration.
MA5-12786 was used in immunohistochemistry to study the chondrogenic differentiation of human mesenchymal stem cells encapsulated in injectable collagen microspheres
|Hui TY,Cheung KM,Cheung WL,Chan D,Chan BP||Biomaterials (29:3201)||2008|
FBS suppresses TGF-beta1-induced chondrogenesis in synoviocyte pellet cultures while dexamethasone and dynamic stimuli are beneficial.
MA5-12786 was used in immunohistochemistry to investigate the influence of fetal bovine serum on chondrogenesis in cultured cells
|Bilgen B,Orsini E,Aaron RK,Ciombor DM||Journal of tissue engineering and regenerative medicine (1:436)||2008|
Mechanical force-induced midpalatal suture remodeling in mice.
MA5-12786 was used in immunohistochemistry to examine the effect of mechanical stress on skeletal remodeling
|Hou B,Fukai N,Olsen BR||Bone (40:1483)||2007|
Mechanical interlocking of engineered cartilage to an underlying polymeric substrate: towards a biohybrid tissue equivalent.
MA5-12786 was used in immunohistochemistry to evaluate an engineered biohybrid cartilage
|Romito L,Ameer GA||Annals of biomedical engineering (34:737)||2006|
Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+/S252W) mouse.
MA5-12786 was used in immunohistochemistry to investigate the changes of cartilage and bone development in Fgfr2 specific mutation mice
|Wang Y,Xiao R,Yang F,Karim BO,Iacovelli AJ,Cai J,Lerner CP,Richtsmeier JT,Leszl JM,Hill CA,Yu K,Ornitz DM,Elisseeff J,Huso DL,Jabs EW||Development (Cambridge, England) (132:3537)||2005|
Microsurgical anatomy of the lateral walls of the pituitary fossa.
MA5-12786 was used in immunohistochemistry to study the microanatomy of the lateral wall of the pituitary fossa
|Peker S,Kurtkaya-Yapicier O,Kiliç T,Pamir MN||Acta neurochirurgica (147:641)||2005|
Transdifferentiation of smooth muscle cells into chondrocytes in atherosclerotic arteries in situ: implications for diffuse intimal calcification.
MA5-12786 was used in immunohistochemistry to study the role of smooth muscle cell transdifferentiation into chondrocytes in atherosclerotic calcification
|Bobryshev YV||The Journal of pathology (205:641)||2005|
Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells.
MA5-12786 was used in immunohistochemistry to study the developmental potential of embryonic stem-cell-derived mesodermal progenitor cells
|Nakayama N,Duryea D,Manoukian R,Chow G,Han CY||Journal of cell science (116:2015)||2003|
Bone defects in latent TGF-beta binding protein (Ltbp)-3 null mice; a role for Ltbp in TGF-beta presentation.
MA5-12786 was used in immunohistochemistry to study the molecular mechanism of Ltbp-3-mediated regulation of chondrocyte differentiation
|Dabovic B,Chen Y,Colarossi C,Zambuto L,Obata H,Rifkin DB||The Journal of endocrinology (175:129)||2002|
Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates.
MA5-12786 was used in immunohistochemistry to study the mechanisms underlying the growth defect in Grg5 null mice
|Wang WF,Wang YG,Reginato AM,Plotkina S,Gridley T,Olsen BR||Developmental dynamics : an official publication of the American Association of Anatomists (224:79)||2002|
Dedifferentiation of mammalian myotubes induced by msx1.
MA5-12786 was used in immunohistochemistry to study the role of msx1 in the dedifferentiation of mammalian myotubes
|Odelberg SJ,Kollhoff A,Keating MT||Cell (103:1099)||2000|
The mouse palate and its cellular responses to midpalatal suture expansion forces.
MA5-12786 was used in immunocytochemistry to study the cellular responses of the mouse palate to midpalatal suture expansion forces
|Katebi N,Kolpakova-Hart E,Lin CY,Olsen BR||Orthodontics and craniofacial research (15:148)||2012|
Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.
MA5-12786 was used in immunocytochemistry to study the fate of transplanted bone marrow-derived mesenchymal stem cells according to transplantation route in a rat model of spinal cord injury
|Kang ES,Ha KY,Kim YH||Journal of Korean medical science (27:586)||2012|
A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties.
MA5-12786 was used in immunocytochemistry to study the molecular and ultrastructural properties of human bone marrow-derived mesenchymal stem cells
|Karaöz E,Okçu A,Gacar G,Sa¿lam O,Yürüker S,Kenar H||Journal of cellular physiology (226:1367)||2011|
Isolation and in vitro characterisation of dental pulp stem cells from natal teeth.
MA5-12786 was used in immunocytochemistry to characterize the biological properties and differentiation potential of human natal dental pulp stem cells
|Karaöz E,Do¿an BN,Aksoy A,Gacar G,Akyüz S,Ayhan S,Genç ZS,Yürüker S,Duruksu G,Demircan PC,Sariboyaci AE||Histochemistry and cell biology (133:95)||2010|
Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.
MA5-12786 was used in immunocytochemistry to study the morphology and immunology of rat bone marrow-derived mesenchymal stem cells
|Karaoz E,Aksoy A,Ayhan S,Sariboyaci AE,Kaymaz F,Kasap M||Histochemistry and cell biology (132:533)||2009|
Characterization of collagenous matrix assembly in a chondrocyte model system.
MA5-12786 was used in immunocytochemistry to study collagenous matrix assembly in a at chondrosarcoma cell line
|Yingst S,Bloxham K,Warner LR,Brown RJ,Cole J,Kenoyer L,Knowlton WB,Oxford JT||Journal of biomedical materials research. Part A (90:247)||2009|
Paracrine and autocrine signals promoting full chondrogenic differentiation of a mesoblastic cell line.
MA5-12786 was used in immunocytochemistry to study how paracrine and autocrine signals combine to promote full chondrogenic differentiation of a mesoblastic cell line
|Locker M,Kellermann O,Boucquey M,Khun H,Huerre M,Poliard A||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (19:100)||2004|
A proteomic analysis of adult rat bone reveals the presence of cartilage/chondrocyte markers.
MA5-12786 was used in western blot to investigate the protein profiles in adult rat bone
|Schreiweis MA,Butler JP,Kulkarni NH,Knierman MD,Higgs RE,Halladay DL,Onyia JE,Hale JE||Journal of cellular biochemistry (101:466)||2007|
Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo.
MA5-12786 was used in western blot to study the molecular and cellular characteristics of processed lipoaspirate cells during chondrogenic differentiation in vitro and cartilage formation in vivo
|Lin Y,Luo E,Chen X,Liu L,Qiao J,Yan Z,Li Z,Tang W,Zheng X,Tian W||Journal of cellular and molecular medicine (9:929)||2005|
Tissue engineered cartilage on collagen and PHBV matrices.
MA5-12786 was used in western blot to study the use of collagen-calcium phosphate and PHBV matrices for tissue engineering of cartilage
|Köse GT,Korkusuz F,Ozkul A,Soysal Y,Ozdemir T,Yildiz C,Hasirci V||Biomaterials (26:5187)||2005|
Galectin-3 is a downstream regulator of matrix metalloproteinase-9 function during endochondral bone formation.
MA5-12786 was used in western blot to study the role of galectin-3 in regulating MMP-9 function during endochondral bone formation
|Ortega N,Behonick DJ,Colnot C,Cooper DN,Werb Z||Molecular biology of the cell (16:3028)||2005|