|Tested species reactivity||Chicken, Human, Mouse|
|Published species reactivity||Rabbit, Mouse, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A purified preparation of lathyritic type II collagen from embryonic chicken sternum.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21807 detects Type II Collagen in Human, Mouse and Chicken samples.
MA1-21807 has been successfully used in Immunofluorescence and western blot procedures. Positive control:cartilage
MA1-21807 immunogen corresponds to A purified preparation of lathyritic type II collagen from embryonic chicken sternum.
This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The balance of WNT and FGF signaling influences mesenchymal stem cell fate during skeletal development.
MA1-21807 was used in immunohistochemistry to investigate the effect of WNT and FGF signaling on mesenchymal stem cell differentiation
|Maruyama T,Mirando AJ,Deng CX,Hsu W||Science signaling (3:null)||2010|
Quantitative proteomics analysis of chondrogenic differentiation of C3H10T1/2 mesenchymal stem cells by iTRAQ labeling coupled with on-line two-dimensional LC/MS/MS.
MA1-21807 was used in western blot to investigate the mechanisms of chondrogenic differentiation of C3H10T1/2 mesenchymal stem cells
|Ji YH,Ji JL,Sun FY,Zeng YY,He XH,Zhao JX,Yu Y,Yu SH,Wu W||Molecular and cellular proteomics : MCP (9:550)||2010|
Expression of exogenous or endogenous green fluorescent protein in adipose tissue-derived stromal cells during chondrogenic differentiation.
MA1-21807 was used in western blot to evaluate the utility of green fluorescent protein in the study of chondrogenic differentiation
|Lin Y,Tian W,Chen X,Yan Z,Li Z,Qiao J,Liu L,Tang W,Zheng X||Molecular and cellular biochemistry (277:181)||2005|
Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys.
MA1-21807 was used in immunocytochemistry to characterize human umbilical cord stroma cells
|Karahuseyinoglu S,Cinar O,Kilic E,Kara F,Akay GG,Demiralp DO,Tukun A,Uckan D,Can A||Stem cells (Dayton, Ohio) (25:319)||2007|
Effects of crystallization of polyhydroxyalkanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes.
MA1-21807 was used in immunocytochemistry to study the physicochemical and biological properties of polyhydroxyalkanoate polymers with potential use in tissue bioengineering
|Zheng Z,Bei FF,Tian HL,Chen GQ||Biomaterials (26:3537)||2005|