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|Tested species reactivity||Amphibian, Chicken, Human, Mouse|
|Published species reactivity||Rabbit, Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13026 targets Collagen II in IF and IHC (P) applications and shows reactivity with Amphibian, Chicken, Human, and mouse samples.
The MA5-13026 immunogen is purified type II collagen; obtained from the adult chicken sternum by limited pepsinization.
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Supplementation with platelet-rich plasma improves the in vitro formation of tissue-engineered cartilage with enhanced mechanical properties.
MA5-13026 was used in immunohistochemistry to study the beneficial effects of platelet-rich plasma supplementation in the generation of tissue-engineered cartilage
|Petrera M,De Croos JN,Iu J,Hurtig M,Kandel RA,Theodoropoulos JS||Arthroscopy : the journal of arthroscopic and related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association (29:1685)||2013|
Application of design-based stereology for estimation of absolute volume and surface area of the articular and calcified cartilage compartments of undecalcified human femoral heads.
MA5-13026 was used in immunohistochemistry to study the ability to determine the volume and surface area of articular and normal cartilage in human femoral heads using design-based stereology
|Hartlev LB,Nyengaard JR,Thomsen JS,Boel LW,Stengaard-Pedersen K,Hauge EM||Journal of microscopy (251:133)||2013|
Specification of chondrocytes and cartilage tissues from embryonic stem cells.
MA5-13026 was used in immunohistochemistry to develop methods for the generation of chondrocytes and cartilage-like tissue from embryonic stem cells
|Craft AM,Ahmed N,Rockel JS,Baht GS,Alman BA,Kandel RA,Grigoriadis AE,Keller GM||Development (Cambridge, England) (140:2597)||2013|
Characterization of the annulus fibrosus-vertebral body interface: identification of new structural features.
MA5-13026 was used in immunohistochemistry to study the structural features of the annulus fibrosus-vertebral body interface
|Nosikova YS,Santerre JP,Grynpas M,Gibson G,Kandel RA||Journal of anatomy (221:577)||2012|
Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes.
MA5-13026 was used in immunohistochemistry to perform a biochemical and molecular characterization of the hyaline cartilage generated by co-culturing passaged human and primary bovine chondrocytes
|Taylor DW,Ahmed N,Hayes AJ,Ferguson P,Gross AE,Caterson B,Kandel RA||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (60:576)||2012|
Structural tympanic membrane changes in secretory otitis media and cholesteatoma.
MA5-13026 was used in immunohistochemistry to identify the distribution of collagen in otitis media and cholesteatoma
|Knutsson J,Bagger-Sjöbäck D,von Unge M||Otology and neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology (32:596)||2011|
Multilineage differentiation potential of cells isolated from the human amniotic membrane.
MA5-13026 was used in immunohistochemistry to characterize cells isolated human amniotic membrane
|Díaz-Prado S,Muiños-López E,Hermida-Gómez T,Rendal-Vázquez ME,Fuentes-Boquete I,de Toro FJ,Blanco FJ||Journal of cellular biochemistry (111:846)||2010|
Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures.
MA5-13026 was used in immunohistochemistry to develop a novel method for the chondrogenic differentiation of human bone marrow-derived mesenchymal stem using micropellets and low oxygen tension
|Markway BD,Tan GK,Brooke G,Hudson JE,Cooper-White JJ,Doran MR||Cell transplantation (19:29)||2010|
Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network.
MA5-13026 was used in immunohistochemistry to study the ability of 3-D nanofiber scaffolds to support the chondrogenic and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells
|Hu J,Feng K,Liu X,Ma PX||Biomaterials (30:5061)||2009|
Agrin is highly expressed by chondrocytes and is required for normal growth.
MA5-13026 was used in immunohistochemistry to investigate the role of agrin in normal skeletal growth
|Hausser HJ,Ruegg MA,Brenner RE,Ksiazek I||Histochemistry and cell biology (127:363)||2007|
The axolotl limb: a model for bone development, regeneration and fracture healing.
MA5-13026 was used in immunohistochemistry to study the axolotl limb as a model for bone development and repair
|Hutchison C,Pilote M,Roy S||Bone (40:45)||2007|
Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).
MA5-13026 was used in immunohistochemistry to study the role of laminin-5 in the proliferation and chondrogenic differentiation of human mesenchymal stem cells
|Hashimoto J,Kariya Y,Miyazaki K||Stem cells (Dayton, Ohio) (24:2346)||2006|
Selection of cell source for ligament tissue engineering.
MA5-13026 was used in immunohistochemistry to optimize the cell source for ligament tissue engineering
|Ge Z,Goh JC,Lee EH||Cell transplantation (14:573)||2005|
Stabilized autologous fibrin-chondrocyte constructs for cartilage repair in vivo.
MA5-13026 was used in immunohistochemistry to develop stabilized fibrin-chondrocyte constructs for in vivo cartilage reconstruction
|Fussenegger M,Meinhart J,Höbling W,Kullich W,Funk S,Bernatzky G||Annals of plastic surgery (51:493)||2003|
Immortalization of human articular chondrocytes and induction of their phenotype.
MA5-13026 was used in immunohistochemistry to immortalize human articular chondrocytes using gene transfection and maintain a stable phenotype after induction
|He Q,Li Q,Yang L,Xu J||Chinese medical journal (116:1351)||2003|
Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells.
MA5-13026 was used in immunohistochemistry to study the developmental potential of embryonic stem-cell-derived mesodermal progenitor cells
|Nakayama N,Duryea D,Manoukian R,Chow G,Han CY||Journal of cell science (116:2015)||2003|
Trypsin-mediated enzymatic degradation of type II collagen in the human vitreous.
MA5-13026 was used in western blot to study the potential role of trypsin-1 and trypsin-2 in the proteolytic degradation of human vitreous type II collagen
|van Deemter M,Kuijer R,Harm Pas H,Jacoba van der Worp R,Hooymans JM,Los LI||Molecular vision (19:1591)||2013|
Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate.
MA5-13026 was used in western blot to study the use of a calcium polyphosphate substrate for the tissue engineering of nuclear pulposus tissue
|Séguin CA,Grynpas MD,Pilliar RM,Waldman SD,Kandel RA||Spine (29:1299)||2004|
Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells.
MA5-13026 was used in immunocytochemistry to study the origins and characteristics of dental, thymic, and bone marrow mesenchymal cells and the corresponding stem cells
|Komada Y,Yamane T,Kadota D,Isono K,Takakura N,Hayashi S,Yamazaki H||PloS one (7:null)||2012|
Cytokine-induced increases in ADAMTS-4 messenger RNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice.
MA5-13026 was used in immunocytochemistry to study the lack of correlation between cytokine-induced ADAMTS-4 mRNA levels and aggreganase proteolytic activity in ADAMTS-5-deficient chondrocytes
|Rogerson FM,Chung YM,Deutscher ME,Last K,Fosang AJ||Arthritis and rheumatism (62:3365)||2010|
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
MA5-13026 was used in immunocytochemistry to evaluate the usefulness of bovine endosteum-derived particles in stem cell culture
|Nigro J,White JF,Ramshaw JA,Haylock DN,Nilsson SK,Werkmeister JA||Biomaterials (31:5689)||2010|
Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine.
MA5-13026 was used in immunocytochemistry to study the use of self-assembling collagen-human mesenchymal stem cell microspheres as novel cell delivery devices in regenerative medicine
|Chan BP,Hui TY,Yeung CW,Li J,Mo I,Chan GC||Biomaterials (28:4652)||2007|
alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha-1 polypeptide; Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; Collagen alpha-1(II) chain; collagen II; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); disproportionate micromelia; procollagen alpha 1(II); procollagen, type II, alpha 1
ANFH; AOM; COL11A3; Col2; Col2a; Col2a-1; COL2A1; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1