|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:4000|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:100-1:1000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human collagen type III protein.|
|Contains||15mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-22147 specifically recognizes native and denatured collagen type III in human and rat samples. It does not recognize collagen types I, II, IV, V, VI and X.
MA1-22147 has been successfully used in ELISA, immunohistochemistry (frozen) and Western blot procedures. By Western blot, this antibody detects a ~70 kDa band in reduced denatured samples, and a ~300 kDa band in non-reduced, non-denatured samples.
The MA1-22147 immunogen is human collagen type III protein.
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV , and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha1 (III) collagen; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; procollagen, type III, alpha 1
Gene Aliases: COL3A1; EDS4A
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