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|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human collagen type III protein.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:4000|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22147 specifically recognizes native and denatured collagen type III in human and rat samples. It does not recognize collagen types I, II, IV, V, VI and X.
MA1-22147 has been successfully used in ELISA, immunohistochemistry (frozen) and Western blot procedures. By Western blot, this antibody detects a ~70 kDa band in reduced denatured samples, and a ~300 kDa band in non-reduced, non-denatured samples.
The MA1-22147 immunogen is human collagen type III protein.
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV , and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phototherapy with low-level laser affects the remodeling of types I and III collagen in skeletal muscle repair.
MA1-22147 was used in immunohistochemistry to investigate the effect of low-level laser phototherapy on collagen remodeling during skeletal muscle repair
|de Souza TO,Mesquita DA,Ferrari RA,Dos Santos Pinto D,Correa L,Bussadori SK,Fernandes KP,Martins MD||Lasers in medical science (26:803)||2011|
Angiotensin-(1-7) ameliorates myocardial remodeling and interstitial fibrosis in spontaneous hypertension: role of MMPs/TIMPs.
MA1-22147 was used in western blot to investigate the efficacy of angiotensin-(1-7) for the treatment of spontaneous hypertension-induced cardiac remodeling and interstitial fibrosis
|Pei Z,Meng R,Li G,Yan G,Xu C,Zhuang Z,Ren J,Wu Z||Toxicology letters (199:173)||2010|
alpha1 (III) collagen; collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; procollagen, type III, alpha 1