Immunohistochemical analysis of Collagen III in formalin-fixed, paraffin-embedded liver tissue using a Collagen III polyclonal antibody (Product # PA1-28870) at a dilution of 1:500 for 4°C for 24 hrs and detected using a Peroxidase biotin-streptavidin rabbit secondary antibody at a dilution of 1:10,000 for 45 min at room temperature. A: Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval was not required. Collagen type I is intra and extracellular. Staining: 3.3 and quote;-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type III from human and bovine placenta|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. NOTE: The preparation of a specific antibody to Collagen III's uninterrupted ""Glycine-X-Y"" triplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in a Western blot using non-denaturing or "native" conditions.
The PA1-28870 immunogen is Collagen Type III from human and bovine placenta.
Store at 4°C short term. For extended storage add 50% glycerol, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.