Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein fragment corresponding to a region within amino acids 1180 and 1444 of Human COL3A1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.46 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol, 1% BSA |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2545304 |
Recommended positive controls: HeLa, COL3A1 shRNA-transfected HeLa, SK-N-SH, SK-N-AS, Neuro2A, NIH-3T3, BCL-1, Raw264.7, C2C12, Mouse ESC, PC-12, Rat2, rat liver.
Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (80%), Pig (94%), Chicken (83%), Rhesus Monkey (98%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704; minisatellite 10w detected by probe MMS10; procollagen, type III, alpha 1
Gene Aliases: AW550625; Col3a-1; COL3A1; EDS4A; mKIAA4231; MMS10-W; Ms10w
UniProt ID: (Human) P02461, (Mouse) P08121, (Rat) P13941
Entrez Gene ID: (Human) 1281, (Mouse) 12825, (Rat) 84032
Molecular Function:
extracellular matrix structural protein
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