Invitrogen

Collagen III Polyclonal Antibody

Product Image Gallery

Collagen III Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (Product # PA5-27828) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Collagen III Antibody in Western Blot (WB) — Western blot analysis of COL3A1 using A) 30 µg Neuro2A whole lysate (B) 30 µg NIH-3T3 whole cell lysate (C) 30 µg BCL-1 whole cell lysate (D) 30 µg Raw264.7 whole cell lysate and E) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a COL3A1 polyclonal antibody (Product # PA5-27828) at a dilution of 1:1000.

Collagen III Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 6 ( A ) Gene expression of extracellular matrix (ECM) proteins and MMPs (MMP-1 and MMP-13) in VEC and VIC cultured in 3D in HG versus NG glucose exposure, as evaluated by real-time PCR. The mRNA of investigated molecules was normalized to actin mRNA. The results are presented as fold change versus NG condition for every type of cell and investigated time. N = 3, * p < 0.5, ** p < 0.01. ( B ) Identification of ECM protein in 3D constructs with VEC and VIC, grown in NG or HG for 7 and 14 days. Representative images of laminin alpha4 chain and collagen III in sections obtained from 3D constructs grown in NG and HG for 7 and 14 days. The images were taken with an 10x objective, with filter for DAPI (nuclei-cyan) and filter Alexa 594 (laminin and collagen III-red).

Product Details

PA5-27828

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 1180 and 1444 of Human COL3A1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.46 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545304

Product Specific Information

Recommended positive controls: HeLa, COL3A1 shRNA-transfected HeLa, SK-N-SH, SK-N-AS, Neuro2A, NIH-3T3, BCL-1, Raw264.7, C2C12, Mouse ESC, PC-12, Rat2, rat liver.

Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (80%), Pig (94%), Chicken (83%), Rhesus Monkey (98%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

Published figure using Collagen III polyclonal antibody (Product # PA5-27828) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Chronic High Glucose Concentration Induces Inflammatory and Remodeling Changes in Valvular Endothelial Cells and Valvular Interstitial Cells in a Gelatin Methacrylate 3D Model of the Human Aortic Valve.

Polymers

Ciortan L,Macarie RD,Cecoltan S,Vadana M,Tucureanu MM,Mihaila AC,Droc I,Butoi E,Manduteanu I

PA5-27828 was used in Immunohistochemistry (Frozen) to study the effect of high glucose on valvular cell phenotype and function in a 3D model.

Wed Nov 25 00:00:00 EST 2020

Polymers

Ciortan L,Macarie RD,Cecoltan S,Vadana M,Tucureanu MM,Mihaila AC,Droc I,Butoi E,Manduteanu I

PA5-27828 was used in Immunohistochemistry (Frozen) to study the effect of high glucose on valvular cell phenotype and function in a 3D model.

Wed Nov 25 00:00:00 EST 2020

Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis.

Translational research : the journal of laboratory and clinical medicine

Fang W,Deng Z,Benadjaoud F,Yang C,Shi GP

PA5-27828 was used in Western Blot to show that CatB deficiency improves liver function in NASH mice by suppressing de novo lipogenesis and liver inflammation and fibrosis.

Sat Aug 01 00:00:00 EDT 2020

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA5-27828 was used in Flow cytometry/Cell sorting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 EDT 2019

ACVR1^R206H FOP mutation alters mechanosensing and tissue stiffness during heterotopic ossification.

Molecular biology of the cell

Haupt J,Stanley A,McLeod CM,Cosgrove BD,Culbert AL,Wang L,Mourkioti F,Mauck RL,Shore EM

PA5-27828 was used in Immunohistochemistry to examine the fibroproliferative tissue preceding heterotopic bone and identified pathologic stiffening at this stage of repair.

Tue Jan 01 00:00:00 EST 2019

Bioinformatics

Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704; minisatellite 10w detected by probe MMS10; procollagen, type III, alpha 1

Gene Aliases: AW550625; Col3a-1; COL3A1; EDS4A; mKIAA4231; MMS10-W; Ms10w

UniProt ID: (Human) P02461, (Mouse) P08121, (Rat) P13941

Entrez Gene ID: (Human) 1281, (Mouse) 12825, (Rat) 84032

Molecular Function: extracellular matrix structural protein