Invitrogen

Collagen III Polyclonal Antibody

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Collagen III Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of COL3A1 in paraffin-embedded papillary carcinoma, stromaL cells, using a COL3A1 polyclonal antibody (Product # PA5-27828) at a dilution of 1:250.

Collagen III Antibody in Western Blot (WB) — Western blot analysis of COL3A1 using A) 30 µg Neuro2A whole lysate (B) 30 µg NIH-3T3 whole cell lysate (C) 30 µg BCL-1 whole cell lysate (D) 30 µg Raw264.7 whole cell lysate and E) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a COL3A1 polyclonal antibody (Product # PA5-27828) at a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1180 and 1444 of Human COL3A1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2545304

Product Specific Information

PA5-27828 targets COL3A1 in IHC (P) and WB applications and shows reactivity with Human, Mouse and Rat samples.

The PA5-27828 immunogen is recombinant protein fragment corresponding to a region within amino acids 1180 and 1444 of Human COL3A1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha1 (III) collagen; autosomal dominant; collagen; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; Ehlers-Danlos syndrome type IV; Ehlers-Danlos syndrome type IV, autosomal dominant; fetal; minisatellite 10w detected by probe MMS10; procollagen, type III, alpha 1

Gene Aliases: AW550625; Col3a-1; COL3A1; EDS4A; mKIAA4231; MMS10-W; Ms10w

UniProt ID: (Human) P02461, (Mouse) P08121, (Rat) P13941

Entrez Gene ID: (Human) 1281, (Mouse) 12825, (Rat) 84032

Function(s)